Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera

Gordon, Karl; Crouse, Edwin J.; Bohnert, H. J.; Herrmann, Reinhold G.

doi:10.1007/bf00272860

Cited by 107 publications

(17 citation statements)

References 34 publications

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“…Such symmetrical occurrence of mutational events in inverted repeats is well documented (7,13,17) and is generally believed to result from a copy-correction mechanism which maintains sequence homogeneity in the repeats.…”

Section: Discussionmentioning

confidence: 99%

“…The numerous deletion/addition differences of 100 to 600 base pairs in the C. eugametos and C. moewusii inverted repeats are probably confined to noncoding sequences. Among closely related land plants, such small differences have been reported within and outside the inverted repeat sequence of cpDNA (6,7,21,25) and the limited nucleotide sequence data available indicate that they reside in noncoding sequences (30,32). The 330 base pair deletion/addition difference mapping within the 23S rDNA region of the C. eugametos and C. moewusii inverted repeats may be due to the presence of an intron of this size in the C. eugarnetos 23S rRNA gene or alternatively to the presence of introns differing by this size in the two algal 23S rRNA genes.…”

Section: Arrangement Of Conserved Sequences In the Inverted Repeats Omentioning

confidence: 99%

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A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of chloroplast DNA from Chlamydomonas eugametos and C. moewusii

1985

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Our recent physical mapping of chloroplast DNA (cpDNA) from Chlamydomonas moewusii, a unicellular green alga which is interfertile with Chlamydomonas eugametos, has revealed a two-fold size difference between the inverted repeat sequences of these algae. With a size of 42 kbp, the inverted repeat of C. moewusii is the largest yet identified in any chloroplast genome. Here we have compared the arrangement of conserved sequences within the two algal inverted repeats by hybridizing cloned restriction fragments representing over 90% of these repeats to Southern blots of cpDNA digests from the two algae. We found that the size difference between the two algal inverted repeats is due to the presence of an extra DNA segment of 21 kilobase pairs (kbp) in C. moewusii. Except for this sequence, the C. moewusii inverted repeat is highly homologous to the entire C. eugametos repeat and the arrangement of conserved sequences in the two repeats is identical. Southern hybridizations with specific gene probes revealed that the conserved sequences include the rDNA region and the genes coding for the large subunit of ribulose 1,5 bisphosphate carboxylase-oxygenase (rbcL) and for the '32 kilodalton' thylakoid membrane protein (psbA). With respect to the conserved sequences, the extra 21 kbp DNA segment of C. moewusii lies in the region of psbA, most probably slightly downstream from this gene.

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Section: Discussionmentioning

confidence: 99%

Section: Arrangement Of Conserved Sequences In the Inverted Repeats Omentioning

confidence: 99%

A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of chloroplast DNA from Chlamydomonas eugametos and C. moewusii

1985

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“…A Southern blot of cpDNA from Oenothera chloroplasts representing plastome types I-IV [7 ] was probed with pOjll9a, which contains a 1.6 kb segment of Oenothera cpDNA that is homologous to ORF2280. The autoradiogram in Fig.…”

Section: Polymorphism Of Orf Sequence In Oenothera Plastomesmentioning

confidence: 99%

“…5. Autoradiogram of Southern hybridization of cpDNA from Oenothera hookeri strain Johansen representing plastome types I-IV [7]. Chloroplast DNA was digested with Bcl I and Barn HI, separated on an agarose gel, blotted to nylon membrane and hybridized to nick-translated pOjll9a.…”

Section: Polymorphism Of Orf Sequence In Oenothera Plastomesmentioning

confidence: 99%

Large unidentified open reading frame in plastid DNA (ORF2280) is expressed in chloroplasts

Glick

Sears

1993

Plant Mol Biol

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The chloroplast DNA encodes genes for components of photosynthesis and the transcription-translation machinery; a number of unidentified open reading frames (ORFs) are also present. To determine whether a large ORF in the inverted repeat of chloroplast DNA of tobacco (ORF2280) encodes a chloroplast protein, a conserved region of the ORF was expressed in Escherichia coli. An antibody against the ORF protein was prepared using the purified fusion protein as an antigen. When incubated with proteins from the soluble fraction of tobacco, spinach and Oenothera chloroplasts, the antiserum detects relatively labile polypeptides, which have apparent molecular weights of 170 to 180 kDa. The ORF in tobacco and spinach is large enough to encode a protein of 240-250 kDa, thus it is possible that post-transcriptional or post-translational processing reduces the size of the expression product. Analysis of Oenothera chloroplasts representing four different plastome types revealed endonuclease restriction fragment length polymorphisms in chloroplast DNA indicative of insertion/deletion events in a region of the chloroplast DNA that shared significant sequence similarity with ORF2280. The ORF2280 antiserum was used to demonstrate that there are qualitative differences in the ORF proteins from different Oenothera plastome types.

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“…The molecular diversification of plastomes has been caused by insertions/deletions, inversions, and base modifications. These events may reflect the co-evolution between plastomes and genomes, as has been shown in the genera Oenothera (5,31) and Nicotiana (4,12,26) by both molecular analysis of plastomes and genetic analysis of interspecific hybrids.…”

Section: Introductionmentioning

confidence: 99%

Polymorphism and gene arrangement among plastomes of ten Epilobium species

Schmitz

Kowallik

1986

Plant Mol Biol

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Plastid DNAs of ten different Epilobium species from four continents have been analysed using the restriction endonucleases BamHI, BglI, BglII, EcoRI, PstI, PvuII and SalI. With respect to the position of cleavage sites of those enzymes, each species has a specific plastome. Fragment patterns of different species from the same continent show a higher degree of similarity than those from different continents. Physical maps of the circular plastid DNA molecule have been constructed for each of the ten species by localising the cleavage sites of the enzymes BglI, PvuII and SalI. As in most other higher plants, the plastid DNA of Epilobium is segmentally organized into two inverted repeats separated by a large and a small single copy region. In heterologous hybridization experiments using radioactively labelled gene probes, the positions of structural genes coding for the rRNAs and for seven polypeptides have been determined. In contrast to its closest relative, Oenothera, the gene arrangement of Epilobium plastomes has the same order as in spinach. This indicates that changes in gene arrangement may be genus-specific and not the result of one or several events affecting all members of a plant family.

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Physical mapping of differences in chloroplast DNA of the five wild-type plastomes in Oenothera subsection Euoenothera

Cited by 107 publications

References 34 publications

A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of chloroplast DNA from Chlamydomonas eugametos and C. moewusii

A 21 kilobase-pair deletion/addition difference in the inverted repeat sequence of chloroplast DNA from Chlamydomonas eugametos and C. moewusii

Large unidentified open reading frame in plastid DNA (ORF2280) is expressed in chloroplasts

Polymorphism and gene arrangement among plastomes of ten Epilobium species

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