Physical mapping of wheat-&lt;i&gt;Aegilops longissima&lt;/i&gt; breakpoints in mildew-resistant recombinant lines using FISH with highly repeated and low-copy DNA probes

Biagetti, Marco; Vitellozzi, Francesca; Ceoloni, Carla

doi:10.1139/gen-42-5-1013

Cited by 12 publications

(8 citation statements)

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“…Conventional techniques, such as chromosome pairing analysis, provide useful information, but it is not possible to characterize wheat-Aegilops translocations using only chromosome pairing. C-banding and in situ hybridization are powerful cytological techniques allowing the detection of Aegilops chromatin (Friebe et al 1992(Friebe et al , 1995a(Friebe et al , b, 1996a(Friebe et al , b, 1999Castilho et al 1996;Nasuda et al 1998;Biagetti et al 1999;Linc et al 1999;Wang et al 2000;Cifuentes et al 2006). The only exception is the D genome of Ae.…”

Section: Cytogenetic and Molecular Characterization Of Aegilops Specimentioning

confidence: 99%

“…tauschii chromosomes show complete identity to the D chromosomes of wheat (Gill and Kimber 1974). Biagetti et al (1999) used two highly repeated DNA sequences (pSc119.2 and pAs1) and one low copy 3BS-specific RFLP sequence to physically map Ae. longissima chromatin in wheat recombinant lines carrying Pm13 derived from Ae.…”

Section: Cytogenetic and Molecular Characterization Of Aegilops Specimentioning

confidence: 99%

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Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat

2007

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Wild Aegilops species related to cultivated wheat (Triticum spp.) possess numerous genes of agronomic interest and can be valuable sources of resistance to diseases, pests and extreme environmental factors. These genes can be incorporated into the wheat genome via intergeneric crossing, following, where necessary, the development of chromosome addition and substitution lines from the resulting hybrids. The transfer of a single segment from an alien chromosome can be achieved by translocations. The Aegilops (goatgrass) species, which are the most closely related to wheat, exhibit great genetic diversity, the exploitation of which has been the subject of experimentation for more than a century. The present paper gives a survey of the results achieved to date in the field of wheat-Aegilops hybridisation and gene transfer. The Aegilops genus consists of 11 diploid, 10 tetraploid and 2 hexaploid species. Of these 23 Aegilops species, most of the diploids (Ae. umbellulata Zhuk., Ae. mutica Boiss., Ae. bicornis (Forssk.) Jaub. & Spach, Ae. searsii Feldman & Kislev ex Hammer, Ae. caudata L., Ae. sharonensis Eig, Ae. speltoides Tausch, Ae. longissima Schweinf. & Muschl.) and several polyploids (Ae. ventricosa Tausch, Ae. peregrina (Hack. In J. Fraser) Marie & Weiller, Ae. geniculata Roth, Ae. kotschyi Boiss., Ae.biuncialis L.) have been used to develop wheatAegilops addition lines. Wheat-Aegilops substitution lines were developed using several species, including Ae. umbellulata, Ae. caudata, Ae. tauschii, Ae. speltoides, Ae. sharonensis, Ae. longissima and Ae. geniculata. Translocations carrying genes responsible for useful agronomic traits were developed with Ae. umbellulata, Ae. comosa, Ae. ventricosa, Ae. longissima, Ae. speltoides and Ae. geniculata. A large number of genes were transferred from Aegilops species to cultivated wheat, including those for resistance to leaf rust, stem rust, yellow rust and powdery mildew, and various pests (cereal cyst nematode, root knot nematode, Hessian fly, greenbug). Many molecular markers are linked to these resistance genes. The development of new molecular markers is also underway. There are still many untapped genetic resources in Aegilops species that could be used as resistance sources for plant breeding.

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Section: Cytogenetic and Molecular Characterization Of Aegilops Specimentioning

confidence: 99%

Section: Cytogenetic and Molecular Characterization Of Aegilops Specimentioning

confidence: 99%

Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat

2007

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“…squarrosa #219 Opata M 85 (Gill, Gill and Endo 1993;Hohmann et al 1994;Delaney et al 1995a, b;Mickelson-Young, Endo and Gill 1995;Nelson et al 1995a, b, c;Van Deynze et al 1995;Gill et al 1996a, b;Marino et al 1996;Röder et al 1998a, b;Biagetti et al 1999;Boyko et al 1999;Sarma et al 2000;Qi and Gill 2001;Varshney et al 2001;Cabrera et al 2002;Faris and Gill 2002;Weng and Lazar 2002;Zhou et al 2002; see also http://www.graingenes.org). In some studies, the microsatellite markers studied here had been directly assigned to chromosome intervals using deletions lines (Endo and Gill 1996).…”

Section: Chromosomal Location Of the Locimentioning

confidence: 99%

Factors affecting polymorphism at microsatellite loci in bread wheat [Triticum aestivum (L.) Thell]: effects of mutation processes and physical distance from the centromere

Thuillet¹,

Bataillon²,

Sourdille³

et al. 2003

Theor Appl Genet

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The effects of factors known to influence the level of polymorphism at microsatellite loci were studied using 99 markers and seven lines of bread wheat. Mutational factors as well as indirect selective events shape diversity at these loci. Theory predicts that the selection of favorable alleles should reduce polymorphism at neutral neighboring loci in genomic areas with low recombination rates. In wheat, local recombination rate is positively correlated with physical distance from the centromere. Seventy four loci among the 99 used could be physically located on the chromosome. We studied how the following affected the diversity among a set of inbred lines: the length of the alleles, the motif (CA versus CT), the structure of the loci (perfect versus imperfect) and the chromosomal position of the loci. For each locus, we determined whether the polymorphism observed at a locus was compatible with the Stepwise Mutation Model (SMM) or the Two-Phase Model (TPM). Both the mutation rate and the compatibility with the SMM or the TPM were shown to be variable between loci. Wheat microsatellite loci were found to be more variable when segregating alleles were perfect and had long motifs (composed of many repetitions). Diversity observed at 19 loci was not compatible with the SMM. Loci located in distal regions, with presumably high recombination rates, had longer allele sizes and were more polymorphic than loci located in proximal regions. We conclude that both mutation factors and indirect selective events vary according to the local recombination rate and therefore jointly influence the level of polymorphism at microsatellite loci in wheat.

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“…Cbanding and FISH permit the distinction of the wheat and Aegilops chromosomes involved in wheat-alien translocations, whereas their size and breakpoint positions can be determined by GISH analysis (see Castilho et al 1996;see Friebe et al 1996). Biagetti et al (1999) used two highly repetitve DNA sequences (pSc119.2 and pAs1) and one low copy 3BS-specific RFLP sequence to physically map Ae. longissima chromatin in wheat recombinant lines carrying Pm13 derived from Ae.…”

Section: Production Of Wheat × Aegilops Hybrids Addition and Translomentioning

confidence: 99%

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

Molnár-Láng

Molnár

Szakács

et al. 2013

Genomics of Plant Genetic Resources

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Physical mapping of wheat-<i>Aegilops longissima</i> breakpoints in mildew-resistant recombinant lines using FISH with highly repeated and low-copy DNA probes

Cited by 12 publications

References 0 publications

Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat

Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat

Factors affecting polymorphism at microsatellite loci in bread wheat [Triticum aestivum (L.) Thell]: effects of mutation processes and physical distance from the centromere

Production and Molecular Cytogenetic Identification of Wheat-Alien Hybrids and Introgression Lines

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