Physical mapping shows that the unstable oxytetracycline gene cluster of Streptomyces rimosus lies close to one end of the linear chromosome

Pandža, Kenan; Pfalzer, Guido; Cullum, John; Hranueli, Daslav

doi:10.1099/00221287-143-5-1493

Cited by 59 publications

(77 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The linearity of chromosomes in the streptomycetes was first discovered in Streptomyces lividans ZX7 and subsequently in 13 other species investigated (Yang, 1994;Lezhava et al, 1995;Leblond et al, 1996;Pandza et al, 1997). These chromosomes contain covalently bound terminal proteins (TPs) and terminal inverted repeats (TIRs) of lengths ranging from 24 to 550 kb.…”

Section: Introductionmentioning

confidence: 99%

Instability of artificially circularized chromosomes of Streptomyces lividans

Lin¹,

Chen²

1997

Molecular Microbiology

View full text Add to dashboard Cite

SummaryThe chromosomes of Streptomyces species are linear molecules, containing long terminal inverted repeats and covalently bound terminal proteins. These chromosomes undergo spontaneous deletions of the terminal sequences at high frequencies and become circularized in several cases examined. Artificial circularization of the Streptomyces lividans chromosome was also achieved by targeted recombination in vivo, in which the terminal inverted repeats of the chromosome were connected by a kanamycin resistance gene (aphII ). Under kanamycin selection, the circularized chromosomes harboured tandem amplifications of a 20.2 kb sequence that included the aphII gene flanked by direct repeats and deletions nearby. On release from kanamycin selection, the aphII amplifications and the neighbouring sequences were deleted from the chromosomes, rendering all the cultures kanamycin sensitive. The chloramphenicol resistance gene, which was prone to deletion in wild-type S. lividans, became much more stable in the kanamycin-sensitive derivatives. These results indicate that the telomeres and/or certain terminal sequences may be involved in the structural instability of Streptomyces chromosomes.

show abstract

Section: Introductionmentioning

confidence: 99%

Instability of artificially circularized chromosomes of Streptomyces lividans

Lin¹,

Chen²

1997

Molecular Microbiology

View full text Add to dashboard Cite

show abstract

“…The overall sequences are not circularly permuted as in T2\T4 Lin et al, 1993). The chromosomal ends are fixed and contain long (24-500 kb) inverted repeats Lezhava et al, 1995 ;Lin et al, 1993 ;Pandza et al, 1997 ;Redenbach et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Streptomyces genomes: circular genetic maps from the linear chromosomes

Wang¹,

Chang²,

Lin³

et al. 1999

Microbiology

View full text Add to dashboard Cite

“…Filamentous soil bacteria, Streptomyces, are unusual in carrying an 8-9 Mb linear chromosome: [1][2][3][4][5][6][7] the genome project for Streptomyces coelicolor A3(2), a model strain for Streptomyces genetics, has been recently completed. 8) Streptomyces species are also known to frequently have linear plasmids, which range from 12 to 1700 kb in size.…”

mentioning

confidence: 99%