Physical properties of a barley protein/nano‐clay composite film containing grapefruit seed extract and antimicrobial benefits for packaging of Agaricus bisporus

Abstract

Summary Barley protein (BP) was extracted from barley flour, and a BP film was prepared. To improve the physical properties of the BP film, nano‐clay was incorporated. Among the films prepared, the composite film containing 4% BP/1% Cloisite Na+ had the best physical property. The composite film containing grapefruit seed extract (GSE) was prepared and used as a packaging film for the button mushroom. Packing of the mushroom with this film inhibited microbial growth during storage. After 7 days of storage, packaging of mushrooms with the film containing 0.7% GSE reduced the populations of total aerobic bacteria and yeast and moulds by 0.95 and 0.58 log CFU/g, respectively, compared with the control. These results suggest that the BP/Cloisite Na+ film containing GSE can be used as an environment‐friendly packaging for maintaining mushroom quality during storage.

Edible Coating Using a Chitosan‐Based Colloid Incorporating Grapefruit Seed Extract for Cherry Tomato Safety and Preservation

“…On strawberries coated with a rapeseed protein‐gelatin film containing 1% GSE, total mesophilic aerobes were inhibited by 1.03 log CFU/g after storage at 4 °C for 14 d (Jang and others . Shin and others () reported that when Agaricus bisporus coated with a barley protein/nano‐clay composite film with 0.7% GSE was stored at 4 °C for 7 d, total mesophilic aerobes were inhibited by approximately 90%. However, the use of chitosan colloid incorporating GSE to improve microbiological safety of tomatoes has not been reported.…”

Physical properties and antimicrobial activities of a persimmon peel/red algae composite film containing grapefruit seed extract

“…Reagor et al (14) also reported that GSE had a greater antimicrobial activity on Gram-positive bacteria compared to Gram-negative bacteria. The antimicrobial activity of GSE is mainly due to the fact that GSE can disrupt bacterial membrane and release cytoplasmic content (9,15). Edible films containing GSE have been previously studied in the antimicrobial packaging of various foods (4,7,8,16).…”

“…Measurement of antimicrobial activity Agar diffusion tests were performed to determine the antimicrobial activity of the PP/RA film containing GSE against Escherichia coli O157:H7 and Listeria monocytogenes according to the method of Shin et al (9). Approximately 7 log CFU/mL of E. coli O157:H7 (0.1 mL) and L. monocytogenes (0.1 mL) were plated onto Tryptic soy broth-Bacto agar (Difco, Detroit, MI, USA) and Oxford medium base agar (Difco), respectively.…”

Preparation of a porcine plasma protein composite film and its application

“…The reduction in TS is mainly related to the loss of intermolecular interactions between protein molecules, and the film with GSE had a lower TS than the control film without GSE. 24) However, it should be noted that the decrease in TS with the addition of 1% GSE did not affect much fragility of the PPP film. In contrast, the E values increased from 6.55 to 12.96% as the GSE content increased from 0 to 1.2 g. Similarly, Kanmani and Rhim 25) found that incorporation of GSE increased E value of the agar/GSE composite films.…”

“…The inhibition zones against E. coli O157:H7 and L. monocytogenes increased with increasing GSE contents. Shin et al 24) and Lim et al 26) also reported that inhibition zones of barley protein/Cloisite Na + composite film and Gelidium corneum/nanoclay composite film increased with the increased GSE contents. The PPP film incorporated with 1% GSE had the inhibition zones of 13.3 and 26.5 mm against E. coli O157:H7 and L. monocytogenes, respectively.…”