In this paper, we have experimentally demonstrated a secure 100 Gb/s intensity-modulation and direct-detection transmission over 100 km standard single-mode fiber (SSMF) based on quantum noise stream cipher (QNSC) for the first time. The original 4-level pulse-amplitude modulation (PAM4) data is mapped to M-level data sequence randomly, and quantum noise such as the generated amplified spontaneous emission noise and the shot noise in the channel can mask these adjacent signal levels. The legitimate receiver needs to distinguish not M-level encrypted data but 4-level data with the shared key. In our scheme, a calculated detection failure probability of 98.72% is achieved. Apparently, more quantum noise contributes to higher security but the worse signal to noise ratio. To balance algorithm complexity, transmission performance and security performance, a sparse 1 regularization based on recursive least square (RLS) algorithm is proposed and firstly used in Volterra equalizer. To eliminate the power fading induced by fiber dispersion, a dual-drive Mach-Zehnder modulator is used to achieve single-sideband modulation, and no dispersion compensation is required. By these means, 100 Gb/s PAM4-QNSC signal transmission over 100 km SSMF with the bit error rate (BER) below the 7% overhead hard-decision forward error correction threshold of 3.8 × 10 −3 is achieved, and >59% complexity reduction of Volterra equalizer is realized. Moreover, the measured BER of 150 Gb/s PAM8-QNSC signal transmission over 50 km SSMF could go below the 20% overhead soft-decision forward error correction threshold of 2.0 × 10 −2. The results validate that the proposed scheme is effective to realize low-cost, high-speed (>100 Gb/s), and secure optical fiber transmission in the data center. INDEX TERMS Optical fiber communication, sparse RLS-Volterra equalizer, quantum noise cipher stream.