Physico–chemical and mechanical properties and antibacterial activity of silver/poly(vinyl alcohol)/graphene nanocomposites obtained by electrochemical method

Surudžić, Rade D.; Janković, Ana; Bibić, N.; Vukašinović-Sekulić, Maja; Perić-Grujić, Aleksandra A.; Mišković‐Stanković, Vesna; Park, Soo‐Jin; Rhee, Kyong-Yop

doi:10.1016/j.compositesb.2015.09.029

Cited by 89 publications

(51 citation statements)

References 51 publications

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“…21,22 Moreover, polymeric nanoparticles reduce concerns regarding the cytotoxicity of AgNPs because the gel limits the direct exposure of a patient's cells to AgNPs and release AgNPs slowly. 23,24 Previously, it was reported that encapsulation of AgNPs inside a polymeric nanoparticle did not affect the size and morphology of the nanocomposite. 25 Polymeric nanoparticles are an attractive vehicle for sustained AgNP release during local therapy because of their tunable size, numerous functional groups, thermo-responsiveness, high loading capacity, good stability, biocompatibility, and anionic charge.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of silver nanoparticles encapsulated in poly-<em>N</em>-isopropylacrylamide-based polymeric nanoparticles

Qasim¹,

Udomluck²,

Chang³

et al. 2018

IJN

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In this study, we analyzed the antimicrobial activities of poly- N -isopropylacrylamide (pNIPAM)-based polymeric nanoparticles encapsulating silver nanoparticles (AgNPs). Three sizes of AgNP-encapsulating pNIPAM- and pNIPAM-NH 2 -based polymeric nanoparticles were fabricated. Highly stable and uniformly distributed AgNPs were encapsulated within polymeric nanoparticles via in situ reduction of AgNO 3 using NaBH 4 as the reducing agent. The formation and distribution of AgNPs was confirmed by UV-visible spectroscopy, transmission electron microscopy, and inductively coupled plasma optical emission spectrometry, respectively. Both polymeric nanoparticles showed significant bacteriostatic activities against Gram-negative ( Escherichia coli ) and Gram-positive ( Staphylococcus aureus ) bacteria depending on the nanoparticle size and amount of AgNO 3 used during fabrication.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of silver nanoparticles encapsulated in poly-<em>N</em>-isopropylacrylamide-based polymeric nanoparticles

Qasim¹,

Udomluck²,

Chang³

et al. 2018

IJN

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“…These peaks can be ascribed to the oxidation of silver nanoparticles from the hydrogel on the surface of Pt electrode. [25,41] Since the anodic current density of a peak in cyclic voltammograms is proportional to the concentration of the reactive species, it is evident from Figure 2 that Ag/PVA/Gr obtained from 0.5 mM AgNO3 swelling solution contains nearly six times higher amount of AgNPs than Ag/PVA/Gr from 0.25 mM AgNO3, indicating more successful synthesis of Ag/PVA/Gr from 0.5 mM AgNO3. Another anodic process occurs at potentials more positive than 800 mV, seen as a shoulder with high current densities on the CVs.…”

Section: Cyclic Voltammetrymentioning

confidence: 98%

“…[52] Ag/PVA/Gr hydrogels were shown to have the ability to release silver over prolonged period of time, with burst release during the first couple of days followed by gradual release over several weeks. [24,25] In such a way, Ag/PVA/Gr hydrogel wound dressings can provide strong protection of the wound against infection and prevent biofilm formation upon initial application, but could also serve as part of antibacterial devices for prolonged use via slow release of AgNPs.…”

Section: Biological Propertiesmentioning

confidence: 99%

“…Moreover, in our previous research, [24,25] we found that hydrogels synthesized from 0.25 mM AgNO3 exhibited good antibacterial activity, at the same time having the mildest cytotoxic effect, which is important for the application of these materials as topical wound dressings. Silver is well known to exhibit dosedependent toxicity, therefore it is important to achieve desired antibacterial effect, while maintaining low cytotoxicity of the materials based on silver nanoparticles.…”

Section: Biological Propertiesmentioning

confidence: 99%

“…[23] This was also confirmed for PVA/Gr and Ag/PVA/Gr nanocomposites in our previous work. [21,24,25] Additionally, several studies indicated that graphene possesses a certain degree of antibacterial activity, with the ability to induce membrane rupture and oxidative stress of bacterial cells. [26,27] This could be an important property, especially in the initial period of Ag/PVA/Gr wound dressing application, when Gr presence could enhance the antibacterial properties of AgNPs and help prevent wound infection and biofilm formation.…”

Section: Introductionmentioning

confidence: 99%

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Graphene Based Composite Hydrogel for Biomedical Applications

Nešović¹,

Abudabbus²,

Rhee³

et al. 2017

Croat. Chem. Acta

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In this work, composite hydrogel consisting of poly(vinyl alcohol), graphene and silver nanoparticles, (Ag/PVA/Gr), was prepared by the immobilization of silver nanoparticles (AgNPs) in PVA/Gr hydrogel matrix in two steps. The first step was cross linking of the PVA/Gr colloid solution by the freezing/thawing method, while in the second step, in situ electrochemical method was used to incorporate AgNPs inside the PVA/Gr hydrogel matrix. We used UV-vis spectroscopy, cyclic voltammetry, Raman spectroscopy, DSC analysis, as well as test of cytotoxicity and antibacterial activity, to characterize obtained Ag/PVA/Gr hydrogel. It was shown that graphene-based composite hydrogel with incorporated AgNPs is non toxic biomaterial with antibacterial activity, with a potential for use in biomedical purposes.

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Antibacterial graphene‐based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

Stevanović

Djošić

Janković

et al. 2020

J Biomedical Materials Res

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Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)‐reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X‐ray diffraction, Raman, and X‐ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture‐free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC‐5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.

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Physico–chemical and mechanical properties and antibacterial activity of silver/poly(vinyl alcohol)/graphene nanocomposites obtained by electrochemical method

Cited by 89 publications

References 51 publications

Antimicrobial activity of silver nanoparticles encapsulated in poly-<em>N</em>-isopropylacrylamide-based polymeric nanoparticles

Antimicrobial activity of silver nanoparticles encapsulated in poly-<em>N</em>-isopropylacrylamide-based polymeric nanoparticles

Graphene Based Composite Hydrogel for Biomedical Applications

Antibacterial graphene‐based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering

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