Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold

Utomo, Dwikora Novembri; Mahyudin, Ferdiansyah; Wardhana, Teddy Heri; Purwati, Purwati; Brahmana, Febrian; Gusti, Agrippina Waya Rahmaning

doi:10.1155/2019/8356872

Cited by 14 publications

(11 citation statements)

References 34 publications

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“…A high porosity in the range of 80−90% is optimal for in vitro cellular adhesion and migration along with the exchange of nutrients and oxygen. 37 The printed constructs were then characterized physicochemically and mechanically. The swelling ability and degradability of the constructs are two crucial requirements for the success of a scaffold/matrix in tissue engineering.…”

Section: Discussionmentioning

confidence: 99%

3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

Singh

Bandyopadhyay

Mandal

2019

ACS Appl. Mater. Interfaces

201

151

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Cartilage tissue is deprived of intrinsic self-regeneration capability; hence, its damage often progresses to a chronic condition which reduces the quality of life. Toward the fabrication of functional tissue substitutes, three-dimensional (3D) bioprinting has progressed vastly over the last few decades. However, this progress is challenged by the difficulty in developing suitable bioink materials as most of them require toxic chemical cross-linking. In this study, our goal was to develop a cross-linker-free bioink with optimal rheology for polymer extrusion, aqueous, and nontoxic processing and offers structural support for cartilage regeneration. Toward this, we use the self-gelling ability of silk fibroin blends (Bombyx mori and Philosamia ricini) along with gelatin as a bulking agent. Silk and gelatin interact with each other through entanglement and physical cross-linking. The ink was rheologically and structurally optimized for printing efficiency in printing grid-like structures. The printed 3D constructs show optimal swelling capability, degradability, and compressive strength. Further, the construct supports the growth and proliferation of encapsulated chondrocytes and formation of the cartilaginous extracellular matrix as indicated by the increased sulfated glycosaminoglycan and collagen contents. This was further corroborated by the upregulation of chondrogenic gene expression with minimal hypertrophy of chondrocytes. Additionally, the construct demonstrates in vitro and in vivo biocompatibility. Notably, the ink demonstrates good print fidelity for printing anatomical structures such as the human ear enabled by optimized extrudability at adequate resolution. Altogether, the results indicate that the developed cross-linker-free silk–gelatin polymer-based bioink demonstrated high potential for its 3D bioprintability and application in cartilage tissue engineering.

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Section: Discussionmentioning

confidence: 99%

3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

Singh

Bandyopadhyay

Mandal

2019

ACS Appl. Mater. Interfaces

201

151

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“…It was confirmed that in repairing and protecting damaged cartilage, MSCs can be loaded on the scaffold to successfully generate cartilage (90). MSCs mainly improve cartilage function and repair cartilage damage from two aspects.…”

Section: Regeneration and Repair Signaling Mechanism Of Mscs In Damag...mentioning

confidence: 83%

Treatment and application of stem cells from different sources for cartilage injury: a literature review

Wang¹,

Zhang

Meng³

et al. 2022

Ann Transl Med

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Background and Objective: Cartilage defects and degeneration have a major impact on daily mobility and quality of life for millions of people worldwide. As the most effective seed cells for tissue engineering applications in regenerative medicine, mesenchymal stem cells (MSCs) are pluripotent cells with mesoderm and neural crest origin. The combination of biomaterial scaffolds with stem cells and drugs for cartilage damage repair has brought much hope to the medical field.Methods: We searched and compared the literature on cartilage damage repaired by stem cells through PubMed and Web of Science method, this review summarizes the research progress of mesenchymal stem cells from various tissue sources in repairing articular cartilage injury.

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“…A pore size of 100-300nm provided an environment conducive to cell metabolism. 23 Gelatine solvent possessed a pore size between 58nm and 475nm. 24 A pore 5-200nm in diameter was identified in the alginate solvent.…”

Section: Discussionmentioning

confidence: 99%

Comparative in vitro study of the cytotoxicity of gelatine and alginate to human umbilical cord mesenchymal stem cells

Hendrijantini¹

2019

Dent. J.

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Background: Mesenchymal stem cells (MSCs) and scaffold combination constitute a promising approach currently adopted for tissue engineering. Umbilical cord-derived mesenchymal stem cells (hUC-MSCs) are easily obtained and non-invasive. Gelatine and alginate constitute a biocompatible natural polymer scaffold. At present, a cytotoxicity comparison of gelatine and alginate to hUC-MSCs is not widely conducted Purpose: This study aimed to compare the cytotoxicity of gelatine and alginate in hUC-MSCs in vitro. Methods: Isolation and culture were performed on hUC-MSCs derived from healthy full-term neonates. Flow Cytometry CD90, CD105 and CD73 phenotype characterization was performed in passage 4. 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) colorimetric assay was performed to measure the cytotoxicity. The three sample groups were: (T1) hUC-MSCs with α-MEM (alpha-minimum essential medium) solution as control; (T2) hUC-MSCs with gelatine; (T3) hUC-MSCs with alginate Results: Flow cytometry of hUC-MSCs displayed positive CD90, CD105 and CD73 surface markers. Gelatine and alginate had no effect on the viability of hUC-MSCs and no statistically significant difference (p>0.05) of cytotoxicity between gelatine and alginate to hUC-MSCs. Conclusion: Gelatine and alginate proved to be non-toxic to hUC-MSCs in vitro.

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Physicobiochemical Characteristics and Chondrogenic Differentiation of Bone Marrow Mesenchymal Stem Cells (hBM-MSCs) in Biodegradable Porous Sponge Bovine Cartilage Scaffold

Cited by 14 publications

References 34 publications

3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

3D Bioprinting Using Cross-Linker-Free Silk–Gelatin Bioink for Cartilage Tissue Engineering

Treatment and application of stem cells from different sources for cartilage injury: a literature review

Comparative in vitro study of the cytotoxicity of gelatine and alginate to human umbilical cord mesenchymal stem cells

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