Physicochemical Analysis of Ginger (Zingiber officinale Rosc.) Rhizome along with its TLC, HPLC and HPTLC Profile

Abstract

Introduction: Ginger (Zingiber officinale Rosc.) is a well-known traditional medicinal plant used for therapeutic effects in both Unani and Ayurvedic System of Medicine. It has been founded to have many therapeutic activities such as anti-inflammatory, anti-rheumatic and anti-gout properties. It is also useful in the management of cough, dyspepsia, loss of appetite, relaxed sore-throat, retching, spasms / spasmodic affections of the stomach, vomiting. The present study aimed towards the evaluation of the parameters involved in the determination of the quality and purity of Zingiber officinale Rosc. rhizome and its standardization. Methods: Organoleptic characters, extractive values, ash values, phyto-chemical analysis, TLC, HPTLC, fluorescence analysis and HPLC profile etc. were the parameters used for the standardisation of the test drug. Results: Total ash values, water, alcohol and Ether soluble extractive values and volatile oil percentage was found to be 7.60%, 11.23%, 8.55%, 2.5% and 3.75% respectively. TLC profile of Z. officinale Rosc shows 05 and 13 spots in UV short and exposure in anisaldehyde-sulphuric acid respectively. The HPLC pattern shows 29 peaks and the peak no. 2, 7 and 5 are major peaks having area concentration and retention time as 9.64% at 2.66 min, 10.12% at 3.25 min. and 15.22% at 3.61 min. respectively. Conclusion: The study will provide referential information for the good quality, purity and identification for the future batches of Zingiber officinale Rosc.

Development of a Gastroretentive Polyherbal Formulation and its Standardization

“…[4] The term extraction can be expressed as the critical isolation of active phytoconstituents from the plant material or animal tissues by utilizing the appropriate techniques, identification measures, and chemical investigation. [7,28] Chromatographic and fingerprint investigation plays an imperative part in the qualitative and quantitative valuation of these intricate herbal remedies. It is now possible to obtain a densitogram and an electronic photocopy of the chromatographic fingerprint to spot the occurrence of marker substances in a test sample by High-performance thinlayer chromatography.…”

“…It is now possible to obtain a densitogram and an electronic photocopy of the chromatographic fingerprint to spot the occurrence of marker substances in a test sample by High-performance thinlayer chromatography. [28] The examination of herbal drugs utilizes the preparative and analytical HPTLC owing to its high separation ability. Almost all the phytoconstituents of the herbal products can be analyzed provided that the optimization of the procedure is done including optimization of the stationary phase and mobile phase along with other chromatographic aspects.…”

“…[4] Presently widespread research is being carried out on diverse plant species and their bioactive components. [28] Fingerprinting involves the characterization of pharmacologically active constituents extracted from a crude drug of natural origin using chromatographic Pharmacognosy Research, Vol 14, Issue 4, Oct-Dec, 2022 in various grades such as 71G, 934, 934P, 971, 971P, 974, and 974P. Hydrogels formed from Carbopol are highly permeable to various drugs and tend to swell thereby releasing the entrapped drug through cross-linkages.…”

In- Vitro Antibacterial Activity of Spilanthes Acmella (Akarkara) Extract on Porphyromonas Gingivalis and Aggregatibactor Actinomycetemcomitans

“…It was performed on 20 cm x 10 cm silica gel aluminium plate. Mian et al (2019) Two micro liters of the extract was deposited in a glass twintrough chamber previously saturated with mobile phase vapour for 20 min and hexane: ethyl acetate: formic acid in the ratio of 7:10:0.1 (v/v/v) was used as mobile phase. Mian et al (2019) After development, the plates were dried with a hair dryer and then the spotted TLC plates visualized in the day light and under the UV wavelength (short & long) i.e.…”

“…254 nm, 366 nm respectively. Mian et al (2019) The Rf value (retardation factor) of spots was determined by the given formulae. Mian et al (2019) Rf Value=Distance travelled by spot / Distance travelled by solvent.…”