Physicochemical and in vitro biological validation of food grade secondary oil in water nanoemulsions with enhanced mucus-adhesion properties

Lagreca, Elena; Vecchione, Raffaele; Cicco, Chiara Di; D’Aria, Federica; Rocca, Alessia La; Gregorio, Ezia De; Izzo, Luana; Crispino, Raffaele; Mollo, Valentina; Bedini, Emiliano; Imparato, Giorgia; Ritieni, Alberto; Giancola, Concetta; Netti, Paolo Antonio

doi:10.1016/j.colsurfa.2022.129998

Cited by 2 publications

(2 citation statements)

References 57 publications

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“…The nanoemulsion consisted of an oil-in-water (O/W) core coated with a mucoadhesive chitosan derivative, namely chitosan. The oil-in-water nanoemulsion was obtained by using a high-pressure homogenizer (Microfluidics M110PS, Massachusetts, USA), as previously described [ 16 , 17 , 18 ]. Briefly, 5.8 g of surfactant (egg lecithin) in 24 mL of oil (soybean oil) was used, and the oil phase was loaded with 100 mg of curcumin.…”

Section: Methodsmentioning

confidence: 99%

“…However, it is known that many nutraceuticals, including curcumin, are unstable or poorly water-soluble, affecting their efficacy upon oral administration. Indeed, previous studies have demonstrated that oil in water nanoemulsions that are loaded with curcumin protect it from degradation, improving its bioavailability [ 16 , 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

Rocca

Gregorio

Lagreca

et al. 2023

IJMS

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Current 3D cancer models (in vitro) fail to reproduce complex cancer cell extracellular matrices (ECMs) and the interrelationships occurring (in vivo) in the tumor microenvironment (TME). Herein, we propose 3D in vitro colorectal cancer microtissues (3D CRC μTs), which reproduce the TME more faithfully in vitro. Normal human fibroblasts were seeded onto porous biodegradable gelatin microbeads (GPMs) and were continuously induced to synthesize and assemble their own ECMs (3D Stroma μTs) in a spinner flask bioreactor. Then, human colon cancer cells were dynamically seeded onto the 3D Stroma μTs to achieve the 3D CRC μTs. Morphological characterization of the 3D CRC μTs was performed to assess the presence of different complex macromolecular components that feature in vivo in the ECM. The results showed the 3D CRC μTs recapitulated the TME in terms of ECM remodeling, cell growth, and the activation of normal fibroblasts toward an activated phenotype. Then, the microtissues were assessed as a drug screening platform by evaluating the effect of 5-Fluorouracil (5-FU), curcumin-loaded nanoemulsions (CT-NE-Curc), and the combination of the two. When taken together, the results showed that our microtissues are promising in that they can help clarify complex cancer–ECM interactions and evaluate the efficacy of therapies. Moreover, they may be combined with tissue-on-chip technologies aimed at addressing further studies in cancer progression and drug discovery.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

Rocca

Gregorio

Lagreca

et al. 2023

IJMS

Self Cite

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Biomimetic Oil-in-Water Nanoemulsions as a Suitable Drug Delivery System to Target Inflamed Endothelial Cells

Lagreca,

Caiazzo,

Di Natale

et al. 2024

Nanomaterials

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Currently, the biomimetic approach of drawing inspiration from nature has frequently been employed in designing drug nanocarriers (NCs) of actively target various diseases, ranging from cancer to neuronal and inflammation pathologies. The cell-membrane coating can confer upon the inner nanomaterials a biological identity and the functions exhibited by the cells from which the membrane is derived. Monocyte- and macrophage-membrane-coated nanomaterials have emerged as an ideal delivery system to target inflamed vasculature. Herein, we developed two biomimetic NCs using a human-derived leukaemia monocytic cell line (THP-1), either undifferentiated or differentiated by phorbol 12-myristate 13-acetate (PMA) into adherent macrophage-like cells as membrane sources for NC coating. We employed a secondary oil-in-water nano-emulsion (SNE) as the inner core, which served as an optimal NC for high payloads of lipophilic compounds. Two different biomimetic systems were produced, combining the biomimetic features of biological membranes with the physicochemical and nano-sized characteristics of SNEs. These systems were named Monocyte NEsoSome (M-NEsoSome) and Macrophage NEsoSome (M0-NEsoSome). Their uptake ability was investigated in tumour necrosis factor alfa (TNFα)-treated human umbilical vein endothelial cells (HUVECs), selected as a model of inflamed endothelial cells. The M0 membrane coating demonstrated accelerated internalisation compared with the monocyte coating and notably surpassed the uptake rate of bare NCs. In conclusion, M0-NEsoSome NCs could be a therapeutic system for targeting inflamed endothelial cells and potentially delivering anti-inflammatory drugs in vascular inflammation.

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Physicochemical and in vitro biological validation of food grade secondary oil in water nanoemulsions with enhanced mucus-adhesion properties

Cited by 2 publications

References 57 publications

Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

Colorectal Cancer Bioengineered Microtissues as a Model to Replicate Tumor-ECM Crosstalk and Assess Drug Delivery Systems In Vitro

Biomimetic Oil-in-Water Nanoemulsions as a Suitable Drug Delivery System to Target Inflamed Endothelial Cells

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