Physicochemical and Structural Characterization of Surface Modified Electrospun PMMA Nanofibre

Abstract

Although electrospun poly(methyl methacrylate) (PMMA) may mimic structural features of extracellular matrix, its highly hydrophobic nature causes reduced cell attachment. This study analysed the physicochemical and structural changes of the surface modified PMMA nanofiber. The electrospun PMMA nanofibers (PM) were surface-treated as follows: PM alone, collagen coated-PM (PM-C), UV-irradiated PM (PM-UV), collagen coated UV-irradiated PM (PM-C-UV) and collagen coated-PM crosslinked with genipin (PM-C-GEN). They were subjected to scanning electron microscopy, Fourier transform infrared (FTIR), cell attachment analysis, X-ray photoelectron spectroscopy (XPS), atomic force microscopy and X-ray powder diffraction (XRD). The surface roughness was lower in PM-C-UV group compared to others. Based on FTIR results, all expected functional group were present in all groups. XPS result showed that there are changes in the mass concentration of UV-treated surfaces and in the collagen coated surfaces. All PM groups showed amorphous nature through XRD. UV irradiation and collagen coating were shown to increase PM's functional groups and modify its surface, which contributed to the increased attachment of cells onto the inert PM scaffold. As conclusion, collagen coated UV irradiated PMMA provided a better surface for cell to attach hence are suitable to be used further as scaffold for in vitro model.

Cell Integration with Electrospun PMMA Nanofibers, Microfibers, Ribbons, and Films: A Microscopy Study

“…Xing et al [17] studied the PMMA scaffolds with incorporated hydroxyapatite (HA) for bone tissue regeneration and mineralization. A few studies have used PMMA scaffolds with surface modifications [31], with collagen coating [32], proteins [33] or blended with polycaprolactone (PCL) [34], to improve the wettability of the scaffolds, and therefore, the cell proliferation and biocompatibility [35]. The improved wettability of scaffolds can be achieved via protein-mediated cell adhesion, thus, the hydrophobic properties of electrospun fibers are able to promote cells integration and proliferation [7,8].…”

PVA/PMMA polymer blended composite electrospun nanofibers mat and their potential use as an anti‐biofilm product

“…To determine the degree of crystallinity of CPX and the drug loaded nanofibers mat, XRD patterns were obtained using a diffractometer (Rigaku Miniflex 600, Japan) having an X‐ray wavelength of λ = 1.5406 Å. The scanning range was taken from 2θ = 10°‐ 60° with a counting rate of 2°/min 17 …”

“…Fourier transform‐infrared spectroscopy (FT‐IR) technique was used to determine the characteristic peaks, various bonds, and functional groups present in CPX and drug loaded electrospun nanofibers mat. The spectra were recorded in the range of 1,000–4,000 cm −1 using Perkin–Elmer FT‐IR spectrometer 17 …”

Electrospun Cellulose Fibres and Applications

“…As a renewable bio-based material on the earth, cellulose has become an extensive interest in producing novel polymers and materials (Prasanth et al 2015;Rosenau et al 2006;Young-Mook Lim 2010). Cellulose with biodegradable properties makes its fibre to be utilised in many applications such as biomedical areas, protective clothing, mechanical areas and filtration (Cucolo & Aminuddin 2001;Muhammad Johan Iskandar et al 2018;Razali et al 2018). This homopolysaccharide is produced through β(1-4) glycosidic bonds condensed from linearly connecting D-glucose units as shown in Figure 1.…”

“…The manufactures of polymer nanofibres via electrospinning technique have been perceived as a persuasive system for producing fibres with submicron diameters by electrostatic forces (Bognitzki et al 2001;Muhammad Hariz et al 2014;Razali et al 2018). Many polymers have been effectively electrospun into ultrafine filaments generally in dissolvable arrangement and some in melt structure (Faten Ermala et al 2017;Huang et al 2003;Hutmacher & Dalton 2011).…”