Physicochemical, antimicrobial, and biological properties of White-MTAFlow

Pelepenko, Lauter Eston; Saavedra, Flávia; Antunes, Thiago Bessa Marconato; Bombarda, Gabriela Fernanda; Gomes, Brenda P. F. A.; Zaia, Alexandre Augusto; Camilleri, Josette; Marciano, Marina Angélica

doi:10.1007/s00784-020-03543-7

Cited by 40 publications

(36 citation statements)

References 34 publications

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“…Moreover, tantalum (Ta 5+ ) was detected both in NeoMTA 2 and NeoMTA Plus. A recent report described that the incorporation of Ta 5+ as an alternative radiopacifier does not cause discoloration and preserves acceptable values of radiopacity [ 30 ]. Also, Si 4+ release was detected in all ion-releasing materials after setting ( p < 0.05).…”

Section: Discussionmentioning

confidence: 99%

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

et al. 2021

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Objective The present study evaluated the biological effects and biomineralization potential of a new tantalum oxide (Ta2O5)–containing material designed for vital pulp therapy or perforation repair (NeoMTA 2), compared to NeoMTA Plus and Bio-C Repair. Material and methods Human dental pulp stem cells (hDPSCs) were exposed to different eluates from NeoMTA Plus, NeoMTA 2, and Bio-C Repair. Ion release from each material was determined using inductively coupled plasma-optical emission spectrometry (ICP-MS). The biological experiments performed were MTT assays, apoptosis/necrosis assays, adhesion assays, migration assays, morphology evaluation, and reactive oxygen species (ROS) production analysis. Biomineralization was assessed by Alizarin red S staining. Finally, osteo/odontogenic gene expression was determined by real-time quantitative reverse-transcriptase polymerase chain reaction (RT-qPCR). Data were analyzed using one-way ANOVA followed by Tukey’s multiple comparison test. Results NeoMTA 2 displayed a significantly higher calcium release compared to the other materials (p < 0.05). When hDPSCs were cultured in presence of the different material eluates, all groups exhibited similar hDPSC viability and migration rates when compared to untreated cells. Substantial cell attachment and spreading were observed in all materials’ surfaces, without significant differences. hDPSCs treated with NeoMTA 2 displayed an upregulation of ALP, Col1A1, RUNX2 (p < 0.001), ON, and DSPP genes (p < 0.05), and showed the highest mineralization potential compared to other groups (p < 0.001). Finally, the more concentrated eluates from these materials, specially NeoMTA Plus and NeoMTA 2, promoted higher ROS production in hDPSCs compared to Bio-C Repair and control cells (p < 0.001), although these ROS levels did not result in increased cell death. Conclusions The new tantalum oxide (Ta2O5)–containing material shows an adequate cytocompatibility and the ability to promote biomineralization without using chemical osteogenic inducers, showing great potential as a new material for vital pulp therapy. Clinical relevance NeoMTA 2 seems to be a promising material for vital pulp therapy. Further studies considering its biocompatibility and biomineralization potential are necessary.

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Section: Discussionmentioning

confidence: 99%

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

et al. 2021

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“…Testing soluble materials-such as reparative endodontic materials-over agar plates seems inappropriate once solubility halos are observed, indicating possible misleading interpretations [34]. Previous studies reported similar observations when testing hydraulic endodontic materials in contact with agar [67,68,75], reporting a limited antimicrobial effect of the reparative endodontic tested materials (Figure 2-Adapted from cited reference [74]); other studies using other methods such as confocal microscopy in contact with dentin and a mature biofilm [84] also concluded that previous disinfection of the site to be treated with these materials is crucial and mandatory in order to expect a positive clinical outcome. Different storage methods for antimicrobial testing can also obtain varying results; in water, for example, ProRoot MTA showed higher antimicrobial activity than when aged in blood and exhibited significant antimicrobial activity reduction after 7 days [35].…”

Section: Hydraulic Calcium Silicate-based Reparative Materialsmentioning

confidence: 83%

“…However, later studies indicated that the interaction of bismuth oxide with the collagen present in dental structures, together with the irrigating solution used during endodontic treatment of root canal therapy, were the main reasons for tooth pigmentation [62][63][64]. These studies resulted in the replacement of bismuth oxide with other substances such as calcium tungstate, zirconium oxide, and tantalum oxide serving as alternative radiopacifiers in compositions such as Biodentine (Septodont, Saint-Maur-des-Fossés, France), EndoSequence BC RRM Putty (Brasseler, Savannah, GA, USA), MTA Repair HP (Angelus, Londrina, Brazil), and White-MTAFlow (Ultradent Products Inc., South Jordan, UT, USA) [32,[65][66][67][68].…”

Section: Hydraulic Calcium Silicate-based Reparative Materialsmentioning

confidence: 99%

Antimicrobial Activity of Calcium Silicate-Based Dental Materials: A Literature Review

et al. 2021

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Endodontic biomaterials have significantly improved dental treatment techniques in several aspects now that they can be used for vital pulp treatments, as temporary intracanal medication, in definitive fillings, in apical surgeries, and for regenerative procedures. Calcium silicate-based cement is a class of dental material that is used in endodontics in direct contact with the dental structures, connective tissue, and bone. Because the material interacts with biological tissues and stimulates biomineralization processes, its properties are of major importance. The main challenge in endodontic treatments is the elimination of biofilms that are present in the root canal system anatomical complexities, as it remains even after chemical-mechanical preparation and disinfection procedures. Thus, an additional challenge for these biomaterials is to exert antimicrobial activity while maintaining their biological properties in parallel. This article reviews the literature for studies considering the antimicrobial properties of calcium silicate-based dental biomaterials used in endodontic practice. Considering the reviewed studies, it can be affirmed that the reduced antimicrobial effect exhibited by calcium silicate-based endodontic materials clearly emphasizes that all clinical procedures prior to their use must be carefully performed. Future studies for the evaluation of these materials, and especially newly proposed materials, under poly-microbial biofilms associated with endodontic diseases will be necessary.

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“…The absence of a specific ISO standard for hydraulic calcium silicate-based materials warrants the inclusion of previously reported methods in the general selection of tests, and the use of the ISO 6876:2012 standard (for endodontic sealers) to evaluate new compositions 7,9,11,12,18,25,26,27 such as Bio-C Pulpo. The inclusion of MTT cell culture testing with fibroblasts was used as a preliminary test for subcutaneous animal biocompatibility, considering the principles of the 3Rs (Replacement, Reduction, and Refinement) in research.…”

Section: Discussionmentioning

confidence: 99%

“…31 In the present study, the color assessment of the materials using the CIELab formula in contact with dentin used a previously reported method that evaluated MTA Angelus before the manufacturer replaced the bismuth oxide 7 with calcium tungstate in this radiopacifier. The use of the CIELab formula was preferred in the present study to allow comparison with previous studies 7,26 that used this method of analysis. The presence of bismuth oxide reported in that study resulted in considerably low L values and dentin staining in MTA Angelus samples.…”

Section: Discussionmentioning

confidence: 99%

Investigation of a modified hydraulic calcium silicate-based material – Bio-C Pulpo

et al. 2021

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This study evaluated the physicochemical, biological, and antimicrobial properties of a new hydraulic calcium silicate-based modified material, and compared it with MTA Repair HP and MTA Angelus. The materials were assessed regarding color luminosity (L), color change, radiopacity, setting time, and ISO 6876:2012 linear flow. Volumetric filling and volume change were evaluated using microcomputed-tomography (µCT). Chemical characterization after 28 days in Hank's Balanced Salt Solution (HBSS) and pH analysis were also assessed. Biological characterization of cytotoxicity and microbiological assessment were also undertaken. Shapiro-Wilk, ANOVA, Levene and post hoc analyses with Bonferroni correction were performed, adopting a 5% significance level (p <0.05). Bio-C Pulpo exhibited the highest L values after 90 days. All tested materials demonstrated color change during the analyses, and had radiopacity above 5 mm Al. MTA Repair HP set faster than Bio-C Pulpo, whereas the latter had the highest linear flow. MTA Repair HP had the highest volumetric filling in µCT analysis. Bio-C Pulpo showed the highest alkalinity during all tested periods, and the highest volumetric loss (above 9%), in comparison with MTA Repair HP and MTA Angelus. Bio-C Pulpo did not form calcium hydroxide after hydration. MTA Repair HP demonstrated the highest cytocompatibility, and Bio-C Pulpo, the highest cytotoxicity. No inhibition halos were observed for any material, and similar higher turbidity values were seen after direct contact. Composition additives used in Bio-C Pulpo modified its properties, and both the absence of calcium hydroxide deposition after hydration, and the related cytotoxicity of this material are of particular concern.

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Physicochemical, antimicrobial, and biological properties of White-MTAFlow

Cited by 40 publications

References 34 publications

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

Biomineralization potential and biological properties of a new tantalum oxide (Ta2O5)–containing calcium silicate cement

Antimicrobial Activity of Calcium Silicate-Based Dental Materials: A Literature Review

Investigation of a modified hydraulic calcium silicate-based material – Bio-C Pulpo

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