Physicochemical changes in human high-density lipoproteins (HDL) oxidized by gamma radiolysis-generated oxyradicals. Effect on their cholesterol effluxing capacity

Bonnefont‐Rousselot, Dominique; Motta, Claude; Khalil, A.; Solà, Rosa; Ville, A.E. La; Delattre, Jacques; Gardès-Albert, Monique

doi:10.1016/0005-2760(94)00211-g

Cited by 74 publications

(42 citation statements)

References 35 publications

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“…This cholesterol capture is broadly known as cholesterol efflux, and the main cell types involved in it are the macrophages under the endothelium in blood vessels. 8 This HDL functional property has been broadly tested in several macrophage cell lines and has been inversely related to early atherosclerosis development and to high risk of experiencing a coronary event.…”

Section: Discussionmentioning

confidence: 99%

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Olive Oil Polyphenols Enhance High-Density Lipoprotein Function in Humans

Hernáez

Fernández‐Castillejo

Farràs

et al. 2014

ATVB

138

154

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Objective-Olive oil polyphenols have shown beneficial properties against cardiovascular risk factors. Their consumption has been associated with higher cholesterol content in high-density lipoproteins (HDL). However, data on polyphenol effects on HDL quality are scarce. We, therefore, assessed whether polyphenol-rich olive oil consumption could enhance the HDL main function, its cholesterol efflux capacity, and some of its quality-related properties, such HDL polyphenol content, size, and composition. Approach and Results-A randomized, crossover, controlled trial with 47 healthy European male volunteers was performed.Participants ingested 25 mL/d of polyphenol-poor (2.7 mg/kg) or polyphenol-rich (366 mg/kg) raw olive oil in 3-week intervention periods, preceded by 2-week washout periods. HDL cholesterol efflux capacity significantly improved after polyphenol-rich intervention versus the polyphenol-poor one (+3.05% and −2.34%, respectively; P=0.042). Incorporation of olive oil polyphenol biological metabolites to HDL, as well as large HDL (HDL 2 ) levels, was higher after the polyphenol-rich olive oil intervention, compared with the polyphenol-poor one. Small HDL (HDL 3 ) levels decreased, the HDL core became triglyceride-poor, and HDL fluidity increased after the polyphenol-rich intervention. Conclusions-Olive

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Section: Discussionmentioning

confidence: 99%

“…The fluidity of the HDL particle was measured based on the determination of the steady-state anisotropy of 1,6-diphenyl-1,3,5-hexatriene (DPH), as previously described (8). In brief, HDL fractions were incubated with DPH 1µM for 30 minutes at room temperature in constant agitation.…”

Section: Hdl Fluidity Determinationmentioning

confidence: 99%

Olive Oil Polyphenols Enhance High-Density Lipoprotein Function in Humans

Hernáez

Fernández‐Castillejo

Farràs

et al. 2014

ATVB

138

154

View full text Add to dashboard Cite

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“…ApoE-poor HDL was oxidized as outlined above for LDL, by incubation with 10 M copper. Extent of oxidation was monitored by fluorescence (excitation: 360 nm; emission: 430 nm) (23,41), yielding a value 6.5-fold greater than native HDL.…”

Section: Methodsmentioning

confidence: 99%

Oxidation of Low Density Lipoproteins Greatly Enhances Their Association with Lipoprotein Lipase Anchored to Endothelial Cell Matrix

Auerbach¹,

Bisgaier²,

Wölle³

et al. 1996

Journal of Biological Chemistry

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“…HDL peroxidation was monitored continuously at 234 nm to detect the formation of conjugated diene, as described previously (17). Thiobarbituric acid-reactive substances, mainly malondialdehyde, were assayed by HPLC coupled to fluorescence detection (25).…”

Section: Conjugated Diene and Thiobarbituric Acid-reactive Substance mentioning

confidence: 99%

“…These changes may affect the ability of HDL particles to accommodate FC molecules that have desorbed from peripheral cells. Additionally, oxidative modifications of HDL affect its capacity to promote cholesterol efflux (17). Indeed, the formation of peroxidation-derived lipid products is associated with changes of the physicochemical properties of HDL and especially a decrease in the fluidity of the HDL phospholipid layer (17).…”

mentioning

confidence: 99%

Age-related impairment of HDL-mediated cholesterol efflux

Berrougui

Isabelle

Cloutier

et al. 2007

Journal of Lipid Research

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Our aim in this study was to investigate the effect of aging on the capacity of HDLs to promote reverse cholesterol transport. HDLs were isolated from plasma of young (Y-HDL) and elderly (E-HDL) subjects. HDL-mediated cholesterol efflux was studied using THP-1 and J774 macrophages. Our results show that E-HDLs present a lower capacity to promote cholesterol efflux than Y-HDLs (41.7 6 1.4% vs. 49.0 6 2.2%, respectively; P 5 0.013). Reduction in the HDL-mediated cholesterol efflux capacity with aging was more significant with HDL 3 than HDL 2 (Y-HDL 3 , 57.3 6 1% vs. E-HDL 3 , 50.9 6 2%; P 5 0.012). Moreover, our results show that ABCA1-mediated cholesterol efflux is the more affected pathway in terms of cholesterol-removing capacity. Interestingly, the composition and structure of HDL revealed a reduction in the phosphatidylcholine-sphingomyelin ratio (E-HDL, 32.7 6 2.7 vs. Y-HDL, 40.0 6 1.9; P 5 0.029) and in the phospholipidic layer membrane fluidity in E-HDL compared with Y-HDL as well as an alteration in the apolipoprotein A-I structure and charge. In conclusion, our results shown that E-HDLs present a reduced capacity to promote cholesterol efflux, principally through the ABCA1 pathway, and this may explain the increase of the incidence of cardiovascular diseases observed during aging.-Berrougui, H., M. Isabelle, M. Cloutier, G. Grenier, and A. Khalil. Age-related impairment of HDL-mediated cholesterol efflux. J. Lipid Res. 2007. 48: 328-336.

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Physicochemical changes in human high-density lipoproteins (HDL) oxidized by gamma radiolysis-generated oxyradicals. Effect on their cholesterol effluxing capacity

Cited by 74 publications

References 35 publications

Olive Oil Polyphenols Enhance High-Density Lipoprotein Function in Humans

Olive Oil Polyphenols Enhance High-Density Lipoprotein Function in Humans

Oxidation of Low Density Lipoproteins Greatly Enhances Their Association with Lipoprotein Lipase Anchored to Endothelial Cell Matrix

Age-related impairment of HDL-mediated cholesterol efflux

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