Physicochemical characterization of cyanophage SM-2

Benson, Randall; Martin, Eugene L.

doi:10.1007/bf00454929

Cited by 7 publications

(5 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies have also examined viruses of other cyanobacteria, including those that infect the freshwater organism Microcystis sp. (3,4,11,19,22). Early interest concerned the use of lytic viruses to control cyanobacterial blooms; the study of freshwater cyanophages waned when it became apparent that they would not be useful biological control agents (36).…”

mentioning

confidence: 99%

Marine and Freshwater Cyanophages in a Laurentian Great Lake: Evidence from Infectivity Assays and Molecular Analyses of g20 Genes

Wilhelm

Carberry

Eldridge

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

While it is well established that viruses play an important role in the structure of marine microbial food webs, few studies have directly addressed their role in large lake systems. As part of an ongoing study of the microbial ecology of Lake Erie, we have examined the distribution and diversity of viruses in this system. One surprising result has been the pervasive distribution of cyanophages that infect the marine cyanobacterial isolate Synechococcus sp. strain WH7803. Viruses that lytically infect this cyanobacterium were identified throughout the western basin of Lake Erie, as well as in locations within the central and eastern basins. Analyses of the gene encoding the g20 viral capsid assembly protein (a conservative phylogenetic marker for the cyanophage) indicate that these viruses, as well as amplicons from natural populations and the ballast of commercial ships, are related to marine cyanophages but in some cases form a unique clade, leaving questions concerning the native hosts of these viruses. The results suggest that cyanophages may be as important in freshwater systems as they are known to be in marine systems.

show abstract

mentioning

confidence: 99%

Marine and Freshwater Cyanophages in a Laurentian Great Lake: Evidence from Infectivity Assays and Molecular Analyses of g20 Genes

Wilhelm

Carberry

Eldridge

et al. 2006

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…population when cultured at the higher CO 2 concentration compared to the lower concentration. Furthermore, it is of note that an increase in CO 2 concentration may coincide with a decrease in environmental pH [74], and, at low pH levels, the release of cations from the culture can promote an increase in the host cell surface charge [73]; as a result, this may improve cyanophage stability and increase adsorption [75]. In a study whose findings support this, Cheng et al [70] investigated the effects of elevated (800 µatm) CO 2 partial pressure (pCO 2 ) on cyanophages, and found a 96% increase in cyanophage production rate and a 57% increase in burst size compared to ambient (400 µatm) pCO 2 at various host growth rates.…”

Section: Carbon Dioxidementioning

confidence: 99%

A Review of Cyanophage–Host Relationships: Highlighting Cyanophages as a Potential Cyanobacteria Control Strategy

Grasso¹,

Pokrzywinski

Waechter

et al. 2022

Toxins

View full text Add to dashboard Cite

Harmful algal blooms (HABs) are naturally occurring phenomena, and cyanobacteria are the most commonly occurring HABs in freshwater systems. Cyanobacteria HABs (cyanoHABs) negatively affect ecosystems and drinking water resources through the production of potent toxins. Furthermore, the frequency, duration, and distribution of cyanoHABs are increasing, and conditions that favor cyanobacteria growth are predicted to increase in the coming years. Current methods for mitigating cyanoHABs are generally short-lived and resource-intensive, and have negative impacts on non-target species. Cyanophages (viruses that specifically target cyanobacteria) have the potential to provide a highly specific control strategy with minimal impacts on non-target species and propagation in the environment. A detailed review (primarily up to 2020) of cyanophage lifecycle, diversity, and factors influencing infectivity is provided in this paper, along with a discussion of cyanophage and host cyanobacteria relationships for seven prominent cyanoHAB-forming genera in North America, including: Synechococcus, Microcystis, Dolichospermum, Aphanizomenon, Cylindrospermopsis, Planktothrix, and Lyngbya. Lastly, factors affecting the potential application of cyanophages as a cyanoHAB control strategy are discussed, including efficacy considerations, optimization, and scalability for large-scale applications.

show abstract

“…Major protein molecular weight is observed through protein analysis and is conducted using the SDS-polyacrylamide gel electrophoresis (PAGE) system, which shows the polypeptides profile of each isolate. The major protein molecular weight is obtained from the protein profile which shows the protein mass location (Adolph and Haselkorn 1971;Benson and Martin 1984;Wilson et al 1993). Wilson et al (1993a) (Benson and Martin 1984).…”

Section: Morphologymentioning

confidence: 99%

“…The major protein molecular weight is obtained from the protein profile which shows the protein mass location (Adolph and Haselkorn 1971;Benson and Martin 1984;Wilson et al 1993). Wilson et al (1993a) (Benson and Martin 1984). Another physicochemical property of cyanophages is genome size.…”

Section: Morphologymentioning

confidence: 99%

“…Cation requirement of cyanophages SM-1, SM-2, AS-1 (Benson and Martin 1984) and AN-15 (Mole et al 1997) are determined by dialyzing against distilled and deionized water, divalent cation (Mg 2+ or Mn 2+ or Ca 2+ ) solution and monovalent cation (Na + ) solution. Benson and Martin (1984) demonstrated that SM-1, SM-2 and AS-1 require cations for stability. In that study, cyanophage SM-2 also showed loss of all infectivity after incubation in distilled and deionized water, and divalent cations conferred greater viral stability than monovalent cations.…”

Section: Morphologymentioning

confidence: 99%

See 1 more Smart Citation

Characterization and dynamics of cyanophages from tropical surface waters

Yeo¹

View full text Add to dashboard Cite

Physicochemical characterization of cyanophage SM-2

Cited by 7 publications

References 15 publications

Marine and Freshwater Cyanophages in a Laurentian Great Lake: Evidence from Infectivity Assays and Molecular Analyses of g20 Genes

Marine and Freshwater Cyanophages in a Laurentian Great Lake: Evidence from Infectivity Assays and Molecular Analyses of g20 Genes

A Review of Cyanophage–Host Relationships: Highlighting Cyanophages as a Potential Cyanobacteria Control Strategy

Characterization and dynamics of cyanophages from tropical surface waters

Contact Info

Product

Resources

About