Physicochemical characterization of exopolysaccharides produced by Lactobacillus rhamnosus on various carbon sources

A novel exopolysaccharide produced by Lactobacillus coryniformis NA-3 exhibits antioxidant and biofilm-inhibiting properties in vitro

“…In this work, we observed that EPS yields were very low (6.9 mg/mL), possibly due to culture of NA-3 in MRS broth. Although there are no reports about EPS yield from L. coryniformis, previous works have shown that EPS production from other strains is affected by many factors such as carbon source (46), composition of medium, temperature, and pH (4). Studies have also suggested that carbon source is a significant factor that promoted EPS production and high EPS could be produced in the presence of sucrose (39,47).…”

“…Biofilms are surface-attached extracellular matrices composed of a complex of nucleic acids, proteins, polysaccharides, and lipids. The polysaccharide components play significant roles as virulence factors that mediate pathogenesis or as essential signals in host-pathogen interactions (46,49). The formation of biofilms by pathogenic bacteria can pose a serious public health risk by decreasing bacterial susceptibility to antimicrobial agents and thus improving their defense mechanisms (51).…”

Probiotic characteristics of exopolysaccharides-producingLactobacillusisolated from some traditional Malaysian fermented foods

“…These include immunostimulation (Hidalgo-Cantabrana et al, 2012;G. Vinderola, Perdigón, Duarte, Farnworth, & Matar, 2006), antioxidant activity (Polak-Berecka et al, 2015;Polak-Berecka, Waśko, & Kubik-Komar, 2014), antitumor activity (Nishimura, 2014), and blood cholesterol reduction (Ryan, Ross, Fitzgerald, Caplice, & Stanton, 2015). Even though it is yet to completely understand the physiological role that play in the ecology of the LAB, it is believed that EPS play a vital role in protecting cells of the LAB from environmental hazards and participate in adhesion and biofilm formation (Looijesteijn, Trapet, De Vries, Abee, & Hugenholtz, 2001).…”

IDENTIFIKASI GLUKOSILTRANSFERASE (gtf) PENYANDI EKSOPOLISAKARIDA PADA STRAIN Weisella confusa PROBIOTIK ASAL AIR SUSU IBU (ASI)

“…EPS yang dihasilkan dari BAL telah banyak dilaporkan dengan sejumlah fungsi kesehatan (Caggianiello et al, 2016). Beberapa fungsi kesehatan diantaranya imunostimulasi (Hidalgo-Cantabrana et al, 2012), aktivitas antioksidan (Polak-Berecka et al, 2015), antitumor (Nishimura, 2014) dan penurunan kolesterol darah (Ryan et al, 2015). Selain itu EPS berperan dalam melindungi sel BAL dari lingkungan berbahaya dan mendukung dalam adhesi dan biofilm.…”