Highlights: High glucose content of grewia gum is the result of high starch content. Grewia gum contains highly acetylated uronic acids. Removal of starch has an impact on the physicochemical properties of the extracts. Rheological behaviour of grewia gum is influenced by changes in pH.
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AbstractGums obtained by extraction from the inner bark of stems can be found in association with starch, which must be digested in order to obtain a refined polysaccharide isolate. In the present study, grewia gum obtained from the inner bark of the stems of Grewia mollis was shown to co-exist with starch and the effect of starch digestion on the physicochemical properties of the resultant polysaccharide was evaluated.The gum was extracted by maceration of the inner bark in deionized water and isolated by a combination of filtration, centrifugation and finally precipitation with absolute ethanol to produce the crude grewia gum extract (GG). The presence and content of starch in the gum sample was determined followed by enzymatic digestion of the starch using α-amylase (Termamyl 120L) to give a starch-free extract (GGDS). Physicochemical properties of the extracts such as total carbohydrates, total protein, differential sugar composition, NMR, intrinsic viscosity and rheological behaviour of the samples were evaluated.The GG extract had total carbohydrate content of ~ 60 % out of which 11.8 % was starch, and a protein content of 2.3 %. Samples also contained galacturonic and glucuronic acid which were highly acetylated. Both samples had a higher proportion of galacturonic acid than glucuronic acid and contained rhamnose, arabinose, galactose, glucose and xylose as neutral sugars in varying proportions. Rheological measurements on 2 %w/w dispersions of the extracts show minor differences between both the original extract and the de-starched material but were influenced by changes in pH.
Keywords:Grewia mollis; polysaccharides; uronic acids; neutral sugars, starch 4
IntroductionGrewia mollis is a shrub native to sub-Saharan Africa belonging to the Malvaceae family. It is cultivated in the middle belt region of Nigeria where the inner bark from the stems of the shrub is pulverised and often used as a thickener in various food formulations.This crude extract has previously been identified to contain polysaccharides (Okafor, Furthermore, the high glucose content of the gum coupled with the fact that the gum is obtained by extraction from the inner bark of the plant Grewia mollis, creates the likelihood of starch being present with the gum. In the present study, the starch content of grewia gum (GG) was evaluated and was subsequently enzymatically digested to give a starch-free extract (GGDS). The physicochemical properties of the two extracts were evaluated, which included a comprehensive evaluation of the uronic and neutral sugar composition, and rheological properties. and 60% were prepared by mixing appropriate amounts of two of the procured commercial standards. Termamyl 120L (heat-stable alpha-amylase) was a kind donation f...