Physicochemical characterization of liposomes after ultrasound exposure – Mechanisms of drug release

Evjen, Tove J.; Hupfeld, Stefan; Barnert, Sabine; Fossheim, Sigrid L.; Schubert, Rolf; Brandl, Martin

doi:10.1016/j.jpba.2013.01.043

Cited by 37 publications

(23 citation statements)

References 16 publications

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“…CryoEM can be used to characterize and control the quality of liposome samples [80], including those that are foreseen for use in biomedicine and nanotechology [2,3,100,101]. It also gives detailed information about encapsulation and internalization phenomena, reveal ing the location of the proteins (Figure 6) or nanoparticles [2,102].…”

Section: Characterization Of Liposomes and Proteoliposomes By Cryo-em Methodsmentioning

confidence: 99%

“…Ideally, quantitative answers that can be used for statis tical analysis would be required, e.g. to determine the stabilizing effect of modified lipids and different bilayer compositions [36,111] or the suitability of a formulation for drug delivery [3,100]. Thus, at present, cryoEM has to be combined with other methods (e.g.…”

Section: Characterization Of Liposomes and Proteoliposomes By Cryo-em Methodsmentioning

confidence: 99%

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Proteoliposomes – a system to study membrane proteins under buffer gradients by cryo-EM

Sejwal

Chami

Baumgartner

et al. 2017

Nanotechnology Reviews

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Membrane proteins are vital to life and major therapeutic targets. Yet, understanding how they function is limited by a lack of structural information. In biological cells, membrane proteins reside in lipidic membranes and typically experience different buffer conditions on both sides of the membrane or even electric potentials and transmembrane gradients across the membranes. Proteoliposomes, which are lipidic vesicles filled with reconstituted membrane proteins, provide an ideal model system for structural and functional studies of membrane proteins under conditions that mimic nature to a certain degree. We discuss methods for the formation of liposomes and proteoliposomes, their imaging by cryo-electron microscopy, and the structural analysis of proteins present in their bilayer. We suggest the formation of ordered arrays akin to weakly ordered two-dimensional (2D) crystals in the bilayer of liposomes as a means to achieve high-resolution, and subsequent buffer modification as a method to capture snapshots of membrane proteins in action.

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Section: Characterization Of Liposomes and Proteoliposomes By Cryo-em Methodsmentioning

confidence: 99%

Section: Characterization Of Liposomes and Proteoliposomes By Cryo-em Methodsmentioning

confidence: 99%

Proteoliposomes – a system to study membrane proteins under buffer gradients by cryo-EM

Sejwal

Chami

Baumgartner

et al. 2017

Nanotechnology Reviews

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“…181,182 In vivo studies have reported enhanced therapeutic benefits of combining US and liposomal cytostatic drugs in tumor treatment. 183,184 Liposome sensitivity to the acoustic waves is affected by their lipid composition. For example, the sonosensitivity of HSPC-based (solid) liposomes is lower than that of DOPE-based (fluid) liposomes, the latter being highly sonosensitive, releasing their payload efficiently due to the irreversible disruption of the membrane during sonication.…”

Section: Liposomes Triggered With Ultrasoundmentioning

confidence: 99%

Ultrasound-Responsive Nanocarriers in Cancer Treatment: A Review

Awad

Paul

AlSawaftah

et al. 2021

ACS Pharmacol. Transl. Sci.

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The safe and effective delivery of anticancer agents to diseased tissues is one of the significant challenges in cancer therapy. Conventional anticancer agents are generally cytotoxins with poor pharmacokinetics and bioavailability. Nanocarriers are nanosized particles, a few of which have received clinical approval, which increase the selectivity of anticancer drugs and genes transport to tumors. They are small enough to extravasate into solid tumors where they slowly release their therapeutic load by passive leakage or by biodegradation. Using smart nanocarriers, the rate of release of the entrapped therapeutic(s) can be increased, and greater exposure of the tumor cells to the therapeutics can be achieved when the nanocarriers are exposed to certain internally (enzymes, pH and temperature) or externally applied (light, magnetic field, and ultrasound) stimuli that trigger the release of their load in a safe and controlled manner, spatially and temporally. This review gives a comprehensive overview of recent research findings on the different types of stimuliresponsive nanocarriers and their application in cancer treatment, with a particular focus on ultrasound.

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“…There is a research finding that large fraction of liposomes can retain its multivesicular structure even after drug is released near-completely in vitro indicating diffusion may dominate [46,55]. Furthermore, ultrasound-induced pore formation can help remain size and structure of liposomes unchanged [56]. Therefore, assumption can be made that bupivacaine possibly shows decreased toxicity in the form of liposomes with the protection of lipids.…”

Section: Additional Advantagementioning

confidence: 99%

Advances of Nano-Structured Extended-Release Local Anesthetics

Qin

Huang

et al. 2020

Nanoscale Res Lett

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Extended-release local anesthetics (LAs) have drawn increasing attention with their promising role in improving analgesia and reducing adverse events of LAs. Nano-structured carriers such as liposomes and polymersomes optimally meet the demands of/for extended-release, and have been utilized in drug delivery over decades and showed satisfactory results with extended-release. Based on mature technology of liposomes, EXPAREL, the first approved liposomal LA loaded with bupivacaine, has seen its success in an extended-release form. At the same time, polymersomes has advances over liposomes with complementary profiles, which inspires the emergence of hybrid carriers. This article summarized the recent research successes on nano-structured extended-release LAs, of which liposomal and polymeric are mainstream systems. Furthermore, with continual optimization, drug delivery systems carry properties beyond simple transportation, such as specificity and responsiveness. In the near future, we may achieve targeted delivery and controlled-release properties to satisfy various analgesic requirements.

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Physicochemical characterization of liposomes after ultrasound exposure – Mechanisms of drug release

Cited by 37 publications

References 16 publications

Proteoliposomes – a system to study membrane proteins under buffer gradients by cryo-EM

Proteoliposomes – a system to study membrane proteins under buffer gradients by cryo-EM

Ultrasound-Responsive Nanocarriers in Cancer Treatment: A Review

Advances of Nano-Structured Extended-Release Local Anesthetics

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