Physicochemical characterization of tetraether lipids from Thermoplasma acidophilum. V. Evidence for the existence of a metastable state in lipids with acyclic hydrocarbon chains

Blöcher, Detlef; Gutermann, Raymund; Henkel, Birgit; Ring, Klaus

doi:10.1016/0005-2736(90)90208-6

Cited by 16 publications

(5 citation statements)

References 44 publications

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“…The isoprenoid constituent of archaeal membranes may, therefore, restrict efficient adaptation mechanisms required to maintain fluidity at subfreezing temperatures. This apparent difference observed in growth temperature minima between archaea and bacteria also leads to interesting questions concerning lipid phases and functionality of the archaeal membrane, because the T m of some archaeal membranes is established at −15/−20 °C or even lower (Blocher et al 1990; Dannenmuller et al 2000; Koga 2012). This average T m thus suggests that archaeal membranes can maintain a liquid crystalline phase below 0 °C without the requirement of extensive fluidity enhancing modifications.…”

Section: Introductionmentioning

confidence: 99%

Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure

2017

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The cytoplasmic membrane of a prokaryotic cell consists of a lipid bilayer or a monolayer that shields the cellular content from the environment. In addition, the membrane contains proteins that are responsible for transport of proteins and metabolites as well as for signalling and energy transduction. Maintenance of the functionality of the membrane during changing environmental conditions relies on the cell’s potential to rapidly adjust the lipid composition of its membrane. Despite the fundamental chemical differences between bacterial ester lipids and archaeal ether lipids, both types are functional under a wide range of environmental conditions. We here provide an overview of archaeal and bacterial strategies of changing the lipid compositions of their membranes. Some molecular adjustments are unique for archaea or bacteria, whereas others are shared between the two domains. Strikingly, shared adjustments were predominantly observed near the growth boundaries of bacteria. Here, we demonstrate that the presence of membrane spanning ether-lipids and methyl branches shows a striking relationship with the growth boundaries of archaea and bacteria.Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-017-0939-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure

2017

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“…7 These branched lipids and ether linkages are thought to promote membrane stability at high temperatures. 8,9 …”

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confidence: 99%

Evidence of a Novel Mevalonate Pathway in Archaea

et al. 2014

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Isoprenoids make up a remarkably diverse class of more than 25000 biomolecules that include familiar compounds such as cholesterol, chlorophyll, vitamin A, ubiquinone, and natural rubber. The two essential building blocks of all isoprenoids, isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP), are ubiquitous in the three domains of life. In most eukaryotes and archaea, IPP and DMAPP are generated through the mevalonate pathway. We have identified two novel enzymes, mevalonate-3-kinase and mevalonate-3-phosphate-5-kinase from Thermoplasma acidophilum, which act sequentially in a putative alternate mevalonate pathway. We propose that a yet unidentified ATP-independent decarboxylase acts upon mevalonate 3,5-bisphosphate, yielding isopentenyl phosphate, which is subsequently phosphorylated by the known isopentenyl phosphate kinase from T. acidophilum to generate the universal isoprenoid precursor, IPP.

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“…All DTA/DSC determinations [22,26,27,28,29,30,31,32,33,34] were conducted with hydrated lipids at various degrees of hydration, directly mixed into the analysis pans (details refer to the respective references), without checking planar or spherical membrane formation. Thus, all values presented are related to “hydrated lipid”.…”

Section: Differential Thermal Analysis (Dta) and Differential Scanmentioning

confidence: 99%

“…Phase transitions were measured in detail of MPL extracted from cells grown at different temperatures (39 °C, 49 °C, 59 °C), i.e., with varying pentacyclization (as shown above in Table 1). MPL49 behaves like mixtures of MPL39 and MPL59 [28,29].…”

Section: Differential Thermal Analysis (Dta) and Differential Scanmentioning

confidence: 99%

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The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

Freisleben

2019

IJMS

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The main phospholipid (MPL) of Thermoplasma acidophilum DSM 1728 was isolated, purified and physico-chemically characterized by differential scanning calorimetry (DSC)/differential thermal analysis (DTA) for its thermotropic behavior, alone and in mixtures with other lipids, cholesterol, hydrophobic peptides and pore-forming ionophores. Model membranes from MPL were investigated; black lipid membrane, Langmuir-Blodgett monolayer, and liposomes. Laboratory results were compared to computer simulation. MPL forms stable and resistant liposomes with highly proton-impermeable membrane and mixes at certain degree with common bilayer-forming lipids. Monomeric bacteriorhodopsin and ATP synthase from Micrococcus luteus were co-reconstituted and light-driven ATP synthesis measured. This review reports about almost four decades of research on Thermoplasma membrane and its MPL as well as transfer of this research to Thermoplasma species recently isolated from Indonesian volcanoes.

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Physicochemical characterization of tetraether lipids from Thermoplasma acidophilum. V. Evidence for the existence of a metastable state in lipids with acyclic hydrocarbon chains

Cited by 16 publications

References 44 publications

Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure

Adaptations of archaeal and bacterial membranes to variations in temperature, pH and pressure

Evidence of a Novel Mevalonate Pathway in Archaea

The Main (Glyco) Phospholipid (MPL) of Thermoplasma acidophilum

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