Physicochemical properties and bioactivity of polysaccharides from <i>Isaria cicadae Miquel</i> with different extraction processes: effects on gut microbiota and immune response in mice

Wang, Wan; Xu, Cong; Liu, Zhijing; Gu, Liya; Ma, Jiage; Hou, Juncai; Jiang, Zhanmei

doi:10.1039/d2fo01646j

Cited by 15 publications

(8 citation statements)

References 66 publications

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“…Wang W et al [ 37 ] found that when healthy mice were fed Isaria cicadae Miquel (ICM) fruiting body polysaccharides, the relative abundances of Lactobacillus , Akkermansia , and Bacteroides were found to increase significantly, while that of Clostridium decreased significantly. Some researchers have used black raspberry anthocyanins as a dietary supplement to enhance the growth of Eubacterium rectum , Faecalibacterium prausnitzii , and Lactobacillus and inhibit the growth of Desulfovibrio and Enterococcus [ 38 ].…”

Section: Discussionmentioning

confidence: 99%

Anthocyanins from Opuntia ficus-indica Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production

Zhang

Chang

Shao

et al. 2022

Biology

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Opuntia ficus-indica is rich in a variety of active substances, such as anthocyanins, flavonoids, and polysaccharides. Some studies have shown that anthocyanins extracted from natural plants can regulate intestinal flora. The fruit was used as raw material, and anthocyanins were extracted from it. In vivo experiments were used to study the effect of Opuntia ficus-indica anthocyanins on the mouse intestine by 16S rRNA high-throughput sequencing (NovaSeq 6000 platform) and gas chromatography (hydrogen flame ionization detector (FID)) methods. Microbiota and effects of short-chain fatty acids (SCFAs). The results showed that after feeding anthocyanins, the diversity of intestinal microorganisms in mice was significantly increased (p < 0.05), the ratio of Firmicutes/Bacteroidetes (F/B value) was significantly decreased (p < 0.05), the relative abundances of beneficial bacteria Lactobacillus, Bifidobacterium, Prevotella, and Akkermansia in the intestinal tract of mice were significantly increased (p < 0.05), and the relative abundance of pathogenic bacteria Escherichia-Shigella and Desulfovibrio decreased significantly (p < 0.05). Furthermore, anthocyanins significantly increased the content of short-chain fatty acids in the cecum of mice, among which the content of acetic acid, propionic acid, and butyric acid increased the most. Opuntia ficus-indica anthocyanins can change the microbial diversity and flora composition of the mouse gut and promote the production of short-chain fatty acids. The findings provide a theoretical basis for the use of Opuntia ficus-indica anthocyanins as dietary supplements to regulate human intestinal flora.

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Section: Discussionmentioning

confidence: 99%

Anthocyanins from Opuntia ficus-indica Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production

Zhang

Chang

Shao

et al. 2022

Biology

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“…Immunofluorescence of Tight Junction Protein and Mucoprotein. The immunofluorescence determination of ZO-1, Claudin-1, and MUC-2 in ileum tissue was described by Wang et al 10 Spontaneous fluorescence quench agent was added to the tissue sections and kept for 5 min. Then, 3% bovine serum albumin was added to the tissue sections for incubation for 30 min, and primary antibody (1:100, rabbit polyclonal anti-ZO-1, anti-Claudin-1, anti-MUC-2) was added to the sections and incubated overnight in a wet box at 4 °C.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%

“…Prebiotics are defined as selective fermentation components that can be utilized by the intestinal microbiota and modulate the intestinal microbiota to promote host health. 10 Because the dysbiosis of the intestinal microbiota due to alcohol exposure is often accompanied by nutritional deficiencies, probiotics may have difficulty obtaining desirable metabolic substrate from the intestinal of ALD patients. Therefore, the combined application of probiotics and prebiotics (synbiotics) targeting intestinal microecology may be an effective solution to alleviate ALD.…”

Section: ■ Introductionmentioning

confidence: 99%

Alleviating Effect of Lacticaseibacillus rhamnosus 1.0320 Combined with Dihydromyricetin on Acute Alcohol Exposure-Induced Hepatic Impairment: Based on Short-Chain Fatty Acids and Adenosine 5′-Monophosphate-Activated Protein Kinase-Mediated Lipid Metabolism Signaling Pathway

Wang

Zhao

et al. 2023

J. Agric. Food Chem.

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Excessive drinking has been listed by the World Health Organization as the fifth major risk factor; especially the liver, as the core organ of alcohol metabolism, is prone to organic lesions. Probiotics have received attention due to their bioactivity for liver protection. The beneficial effects of probiotics on hosts are related to their physiological functions. Therefore, based on the concept of second-generation synbiotes, this study explored the protective effects of four dietary polyphenols on the stress tolerance, hydrophobicity, adhesion, and digestive characteristics of L. rhamnosus 1.0320. L. rhamnosus 1.0320 had the best synergistic effect with dihydromyricetin (DMY). Therefore, this combination was selected as a synbiotic supplement to explore the protective effect on acute alcohol exposure-induced hepatic impairment. The results showed that L. rhamnosus 1.0320 combined with DMY restored the intestinal barrier by upregulating short-chain fatty acid levels and activated the adenosine 5′-monophosphate-activated protein kinase-mediated lipid metabolism pathway to inhibit oxidative stress, inflammation, and lipid accumulation in the liver. Furthermore, 10 9 CFU/mouse/d L. rhamnosus 1.0320 and 50 mg/kg/d DMY by gavage were identified as the optimal doses for protection against acute alcohol expose-induced hepatic impairment. This study provides new insights into alleviating acute alcoholic hepatic impairment by targeting intestinal metabolites through the gut-liver axis.

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“…7 Due to the dynamic imbalance between ROS and antioxidant defense, it ultimately leads to oxidative stress and lipid peroxidation in hepatocytes. 8,9 Furthermore, long-term alcohol intake leads to intestinal dysbiosis and accumulation of intestinal endotoxins. Although most of the absorbed ethanol is metabolized in the liver, alcohol dehydrogenase (ADH) is also expressed in intestinal epithelial cells.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Excessive ROS leads to a dynamic imbalance of the antioxidant system centered on the antioxidant regulatory gene Nrf-2 and downregulates downstream antioxidant genes including superoxide dismutase (SOD), catalase (CAT), and glutathione (GSH) . Due to the dynamic imbalance between ROS and antioxidant defense, it ultimately leads to oxidative stress and lipid peroxidation in hepatocytes. , …”

Section: Introductionmentioning

confidence: 99%

Four Different Structural Dietary Polyphenols, Especially Dihydromyricetin, Possess Superior Protective Effect on Ethanol-Induced ICE-6 and AML-12 Cytotoxicity: The Role of CYP2E1 and Keap1-Nrf2 Pathways

Wang

Shang

et al. 2023

J. Agric. Food Chem.

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Polyphenols have received attention as dietary supplements for the relief of alcoholic liver disease (ALD) due to various bioactivities. Ethanol-induced rat small intestinal epithelial cell 6 (IEC-6) and alpha mouse liver 12 (AML-12) cell models were pretreated with four dietary polyphenols with different structures to explore their effects on cytotoxicity and potential protective mechanisms. The results showed that polyphenols had potential functions to inhibit ethanol-induced AML-12 and IEC-6 cell damage and oxidative stress, and restore ethanol-induced IEC-6 permeability and tight junction gene expression. Especially, dihydromyricetin (DMY) had the best protective effect on ethanol-induced cytotoxicity, followed by apigenin (API). Western blot results showed that DMY and API had the best ability to inhibit CYP2E1 and Keap1, and promote nuclear translocation of Nrf2, which might be the potential mechanism by which DMY and API attenuate ethanol-induced cytotoxicity. Moreover, the molecular docking results predicted that DMY and API could bind more tightly to the amino acid residues of CYP2E1 and Keap1, which might be one of the inhibitory modes of dietary polyphenols on CYP2E1 and Keap1. This study provided a rationale for the subsequent protective effect of dietary polyphenols on alcohol-induced liver injury in animal models and provided new clues on bioactive components for ALD-protection based on the gut-liver axis.

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Physicochemical properties and bioactivity of polysaccharides from Isaria cicadae Miquel with different extraction processes: effects on gut microbiota and immune response in mice

Abstract: The effect of different extraction processes on the physicochemical characterization, digestibility, antioxidant and prebiotic activity of Isaria cicadae Miquel fruiting body polysaccharides (ICM) was studied in this paper. Furthermore, the...

Cited by 15 publications

References 66 publications

Anthocyanins from Opuntia ficus-indica Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production

Anthocyanins from Opuntia ficus-indica Modulate Gut Microbiota Composition and Improve Short-Chain Fatty Acid Production

Alleviating Effect of Lacticaseibacillus rhamnosus 1.0320 Combined with Dihydromyricetin on Acute Alcohol Exposure-Induced Hepatic Impairment: Based on Short-Chain Fatty Acids and Adenosine 5′-Monophosphate-Activated Protein Kinase-Mediated Lipid Metabolism Signaling Pathway

Four Different Structural Dietary Polyphenols, Especially Dihydromyricetin, Possess Superior Protective Effect on Ethanol-Induced ICE-6 and AML-12 Cytotoxicity: The Role of CYP2E1 and Keap1-Nrf2 Pathways

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