Physicochemical Properties of Proteomic Ionic Liquids Matrices for MALDI-MS

Abstract

Ionic liquid matrices (ILMs) have great contributions and showed high improvements for the protein analysis using matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS). The physicochemical properties of these materials are important to understand the ILM performance and design the effective ILMs. The present study represented the relationships of the chemical structure and the physicochemical properties of ILMs. Different organic bases for two common organic matrices called 2,5-dihydroxy benzoic acid (DHB) and 3,5-dimethoxy-4-hydroxycinnamic acid (Sinapinic acid, SA) were calculated. The two series showed the same profile for molar refractivity, molar volume, Parachor, index of refraction, polarizability and surface tension. However, ionic liquids based on sinapinic acid showed higher values than DHB for all parameters except index of refraction and surface tension. These parameters may explain the high performance of SA-ILs for protein analysis compared to DHB-ILs. The present results are important for one who is looking for a new design of ILMs. Journal of

Ionic Liquid-Assisted Laser Desorption/Ionization–Mass Spectrometry: Matrices, Microextraction, and Separation

“…Matrix-assisted laser desorption/ionization–mass spectrometry was applied for the analysis of pathogenic microorganisms and their lysates [37,38,39,40,41,42,43,44,45,46], endotoxins [47], proteins [48,49,50,51], phosphopeptides [52], biothiols [53], surfactants [54], lipids [55], metals ions [56,57,58], metallodrugs [59], surface chemistry of nanoparticles functionalized with synthetic ligands [60], intermediates for quantum dots formation [61], and small molecules [62]. The ionization of an analyte may be assisted using organic matrices [63], ILs [64,65,66], or nanoparticles [67]. Ionic liquid matrices (ILMs) show high sensitivity in most cases and offer soft ionization with minimal fragmentation of thermal labile biomolecules.…”

Advanced Multifunctional Materials (2023)

“…5) Organic polymers e.g., conjugated polymers, covalent-organic frameworks (COFs), and intrinsic microporous polymers. 6) Ionic liquids (Ils) [15][16][17][18][19][20][21]. 7) Metallodrugs [22].…”

An Introductory Review on Advanced Multifunctional Materials

“…Organic polymers, such as conjugated polymers, covalent-organic frameworks (COFs) [78][79][80][81][82], and intrinsic microporous polymers (PIMs) [83][84][85] are covered. Other materials such as ionic liquids (ILs) [86][87][88][89][90][91][92]; metallodrugs [93]; and hybrid materials, such as metal-organic frameworks (MOFs) [94][95][96][97] are also reviewed.…”