Physicochemical, rheological, and bioactive properties of exopolysaccharide produced by a potential probiotic Enterococcus faecalis 84B

Exopolysaccharides from Enterococcus faecium and Streptococcus thermophilus: Bioactivities, gut microbiome effects, and fermented milk rheology

“…These rheological properties of skimmed bovine milk, when fermented with EPS-producing bacteria, have hastened the gelation process, resulting in the faster formation of gel networks with solid-like properties. The observed differences in storage and loss moduli values imply that the type of starter cultures and EPS-producing bacteria can significantly influence the rheological properties of milk fermentation as observed in previous studies ( Ali et al, 2023 , Ayyash et al, 2020 ). Nonetheless, a thorough evaluation of the sensory attributes, such as mouth feel, thickness, creaminess, and firmness, of this fermented low-fat bovine milk has to be investigated to fully understand the impact of EPS.…”

“…The cytotoxicity of EPS-LB13 at 250 mg/L on Caco-2 and MCF-7 cells was 81 % and 83 %, respectively, which was similar to that of EPS obtained from S.therompillus ( Li & Shah, 2016 ). In contrast, EPS-MLB10 demonstrated lower cytotoxicity on MCF-7 cells (14 %) than on Caco-2 cells (83 %) which was comparable to the EPS produced by E. faecalis ( Ali et al, 2023 ). The antimicrobial and antibiofilm activities of EPS-LB13 and EPS-MLB10 against common food pathogens were evaluated at three different concentrations (250, 125, and 62.5 mg/L).…”

“…1 b, respectively. The inhibition of both diabetic-causing enzymes increased ( P < 0.05 ) with increasing concentrations of EPS, indicating potential antidiabetic properties by inhibiting the key enzymes involved in carbohydrate digestion and absorption ( Ali et al, 2023 ). The strongest inhibition was observed at 250 mg/L, where EPS-LB13 inhibited α-amylase and α-glucosidase by 59 % and 2 %, respectively, while EPS-MLB10 inhibited them by 57 % and 17 %, respectively.…”

“…The Fourier transform-infrared spectroscopy (FT-IR) analysis of the purified EPS-LB13 and EPS-MLB10 was carried out to determine the functional groups within the EPS structures using previously described methodology ( Ali et al, 2023 ). Briefly, the EPS was placed on a Diamond/ZnSe crystal plate of Spectrum Two FT-IR Spectrometer (PerkinElmer Inc., USA) and FT-IR spectra was obtained by scanning for16 times from 4000 to 400 cm −1 at room temperature (23 ± 0.1 °C) with a scanning resolution of ± 4 cm −1 .…”

Structure and Properties of Exopolysaccharide Produced by Gluconobacter frateurii and Its Potential Applications

“…This region was a specific signal of polysaccharides. A weak band at 2938 cm −1 was associated with the stretching vibration of the C-H bond [34]. In addition, the peaks at 1653 cm −1 and 1451 cm −1 indicated C=O and C-O bond stretching vibrations, respectively [35].…”