Physics of Light and Optics: A Free Online Textbook

Peatross, Justin; Ware, Michael

doi:10.1364/fio.2010.jwa64

Cited by 50 publications

(56 citation statements)

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“…In our case we apply the same P in both lateral directions. By optimizing P and h it should be possible to improve the wavelength dependent reflection and scattering characteristics of the periodic diffraction grating [28]. In the case of scattering, diffraction modes are wavelength dependent.…”

Section: Filtering Structuresmentioning

confidence: 99%

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Modelling of diffraction grating based optical filters for fluorescence detection of biomolecules

Kovačič

Krč

Lipovšek

et al. 2014

Biomed. Opt. Express

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Abstract:The detection of biomolecules based on fluorescence measurements is a powerful diagnostic tool for the acquisition of genetic, proteomic and cellular information. One key performance limiting factor remains the integrated optical filter, which is designed to reject strong excitation light while transmitting weak emission (fluorescent) light to the photodetector. Conventional filters have several disadvantages. For instance absorbing filters, like those made from amorphous silicon carbide, exhibit low rejection ratios, especially in the case of small Stokes' shift fluorophores (e.g. green fluorescent protein GFP with λ exc = 480 nm and λ em = 510 nm), whereas interference filters comprising many layers require complex fabrication. This paper describes an alternative solution based on dielectric diffraction gratings. These filters are not only highly efficient but require a smaller number of manufacturing steps. Using FEM-based optical modelling as a design optimization tool, three filtering concepts are explored: (i) a diffraction grating fabricated on the surface of an absorbing filter, (ii) a diffraction grating embedded in a host material with a low refractive index, and (iii) a combination of an embedded grating and an absorbing filter. Both concepts involving an embedded grating show high rejection ratios (over 100,000) for the case of GFP, but also high sensitivity to manufacturing errors and variations in the incident angle of the excitation light. Despite this, simulations show that a 60 times improvement in the rejection ratio relative to a conventional flat absorbing filter can be obtained using an optimized embedded diffraction grating fabricated on top of an absorbing filter.

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Section: Filtering Structuresmentioning

confidence: 99%

“…By scattering the excitation light only, absorption is increased by prolonging the optical path, while the emisson light does not scatter. Theoretically, this is true since λ exc < λ em and because the scattering process at the grating occurs if λ ≥ P [28], where λ is the wavelength of light in the incident layer.…”

Section: Filtering Structuresmentioning

confidence: 99%

Modelling of diffraction grating based optical filters for fluorescence detection of biomolecules

Kovačič

Krč

Lipovšek

et al. 2014

Biomed. Opt. Express

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“…Geometrical rules are followed to transform the spot areas at certain positions of the screen to the angles and intensities of the ADF. To test the transformation we measured the 3D ADF of a linear periodic grating where the angles of the discrete scattered beams (modes) can be calculated based on the diffraction theory [16]. The results of the measurements are shown in Fig.…”

Section: B Screen Angle and Solid Angle Transformationmentioning

confidence: 99%

Camera-based angular resolved spectroscopy system for spatial measurements of scattered light

2014

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We present a system for the measurement of the three-dimensional (3D) angular distribution function (ADF) of scattered or emitted light using a digital camera. The 3D ADF can be determined from the digital image captured from a reflective flat screen. With the developed camera-based system we can quantify the transmitted light scattered by textured samples or the light emitted from light sources in a few second's time. In the paper, the setup of the camera-based system is presented, the main transformations of the acquired digital image to obtain the 3D ADF are explained, and sensitivity issues are discussed. The system is applied to and validated on randomly nanotextured transparent samples and a calibrated light emitting device. Good matching is obtained with the measurements carried out with a conventional goniometric angular resolved scattering system.

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“…According to [72], the Jones vector can be used only for completely polarized signals, e.g. GNSS signals.…”

Section: Polarization State Of the Gnss Signalsmentioning

confidence: 99%

Multipath Propagation, Characterization and Modeling in GNSS

Smyrnaios¹,

Schön²,

Nicolas³

2013

Geodetic Sciences - Observations, Modeling and Applications

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Physics of Light and Optics: A Free Online Textbook

Cited by 50 publications

References 0 publications

Modelling of diffraction grating based optical filters for fluorescence detection of biomolecules

Modelling of diffraction grating based optical filters for fluorescence detection of biomolecules

Camera-based angular resolved spectroscopy system for spatial measurements of scattered light

Multipath Propagation, Characterization and Modeling in GNSS

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