Physiochemical analysis and HPLC-PDA method for quantification of stigmasterol in Dipteracanthus patulus (Jacq.) Nees

Nachimuthu, Kannikaparameswari; Palaniswamy, Chinnaswamy

doi:10.1016/j.jopr.2013.08.014

Journal of Pharmacy Research

2013

DOI: 10.1016/j.jopr.2013.08.014

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Physiochemical analysis and HPLC-PDA method for quantification of stigmasterol in Dipteracanthus patulus (Jacq.) Nees

Kannikaparameswari Nachimuthu¹,

Chinnaswamy Palaniswamy²

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Cited by 2 publications

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High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

Kunchanur,

Mannur,

Koli

2024

Separation Science Plus

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The primary objective of this investigation was to develop and validate a robust reversed‐phase high‐performance liquid chromatography (RP‐HPLC) method for quantifying stigmasterol in both Moringa oleifera powder and tablets. The analysis was conducted using a Box–Behnken design. Stigmasterol separation was achieved with a Phenomenex Luna C‐18 column (4.6 × 150 mm2, 5 µm) and a mobile phase consisting of methanol and 0.1% formic acid in an 80:20% v/v ratio. The separation was performed at a flow rate of 1 mL/min, and stigmasterol was detected at a wavelength of 210 nm using a photodiode array detector. Method validation followed the guidelines set forth by the International Council for Harmonisation (ICH) Q2 (R1). This rigorous validation process evaluated critical parameters, including linearity, accuracy, system suitability, precision, and robustness. The results for each parameter were well within the acceptable range, confirming the reliability and efficacy of the established RP‐HPLC method. The findings of this study highlight the potential of the RP‐HPLC method as a dependable tool for the routine analysis of stigmasterol in herbal formulations.

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High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

Kunchanur,

Mannur,

Koli

2024

Separation Science Plus

View full text Add to dashboard Cite

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Antioxidant activity of edible sprouts and phytosterol contents by HPLC/UV analysis

Kim

Bang

et al. 2022

Hortic. Environ. Biotechnol.

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This study evaluated the in vitro radical scavenging activities of edible tree sprouts, particularly those of the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), hydroxyl radical, and singlet oxide radical, to assess their antioxidant activities. Additionally, stigmasterol (ST) and β-sitosterol (BS) were analyzed using HPLC/UV. The edible sprouts of Eleutherococcus senticosus (ESC) and Morus alba (MAB) exhibited the highest DPPH scavenging activity among other edible sprouts. A reverse-phase column was used in an isocratic elution system, after which UV detection was performed at 210 nm. ST and BS analyses indicated that ESC sprouts contained the highest amounts of ST (9.99 mg·g− 1 extract), whereas MAB sprouts contained the highest concentrations of BS (14.69 mg·g− 1 extract). In conclusion, the highest antioxidant activity was observed in the edible sprouts with the highest phytosterol content. Therefore, our findings provide a theoretical basis for the development of plant-based functional foods or supplements with antioxidant properties.

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Physiochemical analysis and HPLC-PDA method for quantification of stigmasterol in Dipteracanthus patulus (Jacq.) Nees

Cited by 2 publications

References 9 publications

High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

High‐Performance Liquid Chromatography–Based Standardization of Stigmasterol in Moringa oleifera: Method Development and Validation Through Design of Experiment Approach

Antioxidant activity of edible sprouts and phytosterol contents by HPLC/UV analysis

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