Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus)

Krishnakumar, P K; Asokan, P K; Pillai, V K

doi:10.1016/0166-445x(90)90024-j

Cited by 64 publications

(25 citation statements)

References 28 publications

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“…Lipofuscin seems to be common in molluscs, as it has been reported in situ in various tissues and organs of several species such as the gastropods Maoricrypta monoxyla (Nelson and Morton, 1979) and Aplysia punctata (Taieb and Vicente, 1999) and the bivalves Mytilus galloprovincialis (Viarengo et al, 1987(Viarengo et al, , 1990Regoli, 1992;Sarasquete et al, 1992), Mytilus edulis (Moore et al, 1980;George et al, 1982;Lowe, 1988;Burbidge et al, 1994;Hole et al, 1995;Krishnakumar et al, 1994) and Perna viridis (Krishnakumar et al, 1990;Mathew and Damodaran, 1997). Only a few findings are reported from clams so far: In the alimentary tract epithelium, in gonadal connective tissue and in the renal epithelium of Mya arenaria and Mercenaria mercenaria (Brown and O'Toole, 1978); in the digestive gland of Ruditapes phillipinarum (Sarasquete et al, 1992) and of Sunetta scripta (Mathew and Damodaran, 1997); and in the kidney of Tridacna gigas (Reid et al, 1984).…”

Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 99%

“…Sheehy et al, 1994Sheehy et al, , 1995O'Donovan and Tully, 1996;Wahle et al, 1996;Belchier et al, 1999;Bluhm and Brey, 2001). In molluscs, however, lipofuscin accumulation has been studied primarily in relation to stress (Krishnakumar et al, 1990;Viarengo et al, 1990;Hole et al, 1992Hole et al, , 1993Hole et al, , 1995Mathew and Damodaran, 1997). Our study is the first to show a clear relationship between age and lipofuscin in situ in a bivalve.…”

Section: Can We Use Lipofuscin As a Proxy For Individual Age In E Exmentioning

confidence: 99%

“…Molluscs studies of lipofuscin in situ have focused primarily on the effect of stress such as exposure to heavy metals or anoxic conditions (e.g. Krishnakumar et al, 1990Krishnakumar et al, , 1994Viarengo et al, 1990;Sarasquete et al, 1992;Hole et al, 1992Hole et al, , 1993Hole et al, , 1995Mathew and Damodaran, 1997). The few studies relating lipofuscin to age in molluscs (Clarke et al, 1990;Zielinski and Pörtner, 2000;Sukhotin et al, 2002) are based on solvent extraction of autofluorescent substances.…”

Section: Introductionmentioning

confidence: 99%

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Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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In the hard clam Eurhomalea exalbida, autofluorescent granules were detected in high concentrations in the connective tissue around the intestine and in other tissues. Autofluorescence combined with Sudan black B and PAS positive reactions suggested that these granules were lipofuscin-like. The concentration of this material in the connective tissue (CT) around the intestine was quantified by image analysis and expressed as total area fraction occupied by lipofuscin granules. Lipofuscin concentration was distinctly better related with individual age as determined from stable isotope-validated shell growth bands than with any morphometric parameter. This relationship was described best by a Gompertz model: Lipofuscin t = 24.79e À e À 0.029(Age t À 58.578) (N = 38; r nl 2 = 0.882). Age was predicted from lipofuscin CT concentration by a von Bertalanffy model: Age t = 68.00 (1 À e À 0.146(Lipofuscin t + 0.028) ) 0.664 (N = 38; r nl 2 = 0.933). Our findings suggest that lipofuscin CT concentration in E. exalbida is a function of individual age. If this holds true for bivalves in general, lipofuscin may be a suitable proxy for age in species with less clear shell growth band patterns. D

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Section: Is the Substance Found In E Exalbida Lipofuscin?mentioning

confidence: 99%

Section: Can We Use Lipofuscin As a Proxy For Individual Age In E Exmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Laudien

Schiedek

Brey

et al. 2002

Journal of Experimental Marine Biology and Ecology

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“…Laboratory studies confirm that lysosomal responses in the digestive cells show strong associations to contaminant exposure (Lowe et al 1981;Moore and Clark 1982;Moore et al 1985;Pipe and Moore 1986;Nott and Moore 1987;Axiak et al 1988;Moore 1988Moore , 1991Cajaraville et al 1989;Krishnakumar et al 1990;Winston et al 1991;Viarengo et al 1992). Parameters that were consistently indicative of contaminant exposure included lysosomal stability, nicotinamide adenine dinucleotide phosphate (NADPH)-ferrihemoprotein reductase activity, lipofuscin deposition, and accumulation of lysosomal and cytoplasmic unsaturated neutral lipid.…”

mentioning

confidence: 90%

Assessment of Seasonal Variability of Cytochemical Responses to Contaminant Exposure in the Blue Mussel Mytilus edulis (Complex)

Kagley

Snider

Krishnakumar

et al. 2003

Archives of Environmental Contamination and Toxicology

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Abstract.A selected suite of cytochemical parameters in Mytilus edulis are altered in response to field and laboratory exposure to chemical contaminants. These biomarkers include lysosomal stability, nicotinamide adenine dinucleotide phosphate (NADPH)-ferrihemoprotein reductase activity, liposfuscin deposition, and accumulation of lysosomal and cytoplasmic unsaturated neutral lipid. Normal variations in physiological processes (influenced by exogenous seasonal changes in temperature, salinity, food availability, etc.) may alter the sensitivity of these biomarkers to contaminant exposure. To address this issue, M. edulis (complex) were sampled monthly from a reference nonurban site (Coupeville, Penn Cove) and a polluted urban site (Seacrest, Elliott Bay) in Puget Sound, WA, for a period of 15 months. Physiological measurements including total length, total weight, somatic and mantle weights (an indication of gonadal development and reproductive status), condition index, and the presence or absence of hemic neoplasia (HN, or leukemia) were recorded. Significant differences in lysosomal stability, lysosomal and cytoplasmic unsaturated neutral lipids, lipofuscin deposition, and NADPH-ferrihemoprotein reductase activity in cells of the digestive gland or digestive tubules were generally found in mussels taken throughout the year from Seacrest compared to mussels sampled from Coupeville, consistent with exposure to chemical contaminants. No seasonally influenced suppression of the entire suite of parameters as measures of contaminant exposure was evident. Therefore these biomarkers can be used to evaluate contaminant exposure in mussels throughout the entire year.

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“…viridis ha sido utilizado como herramienta biológica en monitoreos de ecosistemas marinocosteros (Chidambaran, 1996;Krishnakumar et al, 1996). Esta especie tiene la capacidad de responder bioquímicamente en presencia de Cd y otros contaminantes de naturaleza metálica, especialmente mediante la síntesis de metalotioneínas (MT) y activación del sistema antioxidante (Verlecar et al, 2007).…”

Section: Introductionunclassified

Tolerancia a la anoxia y defensas antioxidantes en el mejillon verde Perna viridis (Linneus, 1758) bajo exposicion aguda al cadmio

Zapata-Vívenes

Tovar-Sánchez

Nusetti

et al. 2014

lajar

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Tolerancia a la anoxia y defensas antioxidantes en el mejillón verdeRESUMEN. Se estimó la sobrevivencia a la anoxia del mejillón verde Perna viridis después de siete días de exposición a 50 g L -1 de cadmio (Cd), seguido de un período de recuperación en el mar durante 21 días. En la glándula digestiva de los organismos experimentales se determinó los niveles de Cd acumulados, y marcadores de estrés oxidativo tales como: metalotioneínas (MT), glutatión reducido (GSH), peroxidación lipídica (TBARS) y las actividades de las enzimas catalasa (CAT), glutatión reductasa (GR) y glutatión-S-transferasa (GST). Los organismos expuestos a Cd mostraron una menor tolerancia al tratamiento anóxico, sin embargo una vez recuperados en el mar presentaron valores de sobrevivencia relativamente mayores en comparación a sus controles. Los organismos expuestos a Cd revelaron un incremento en la concentración de MT y la actividad de CAT, correlacionado al ingreso corporal de Cd. Los organismos transplantados al mar mostraron una disminución del metal acumulado, manteniendo niveles de MT incrementados. Los mejillones tratados y controles no exhibieron diferencias significativas en los marcadores de estrés oxidativo determinados (TBARS, GSH, GR y GST). El Cd acumulado posiblemente afectó algunas estrategias bioquímicas relacionadas con la tolerancia a la anoxia de P. viridis. Sin embargo, los altos niveles de MT en organismos pre-expuestos y posteriormente recuperados, podría condicionar la protección contra la toxicidad del Cd e indirectamente una mayor tolerancia a la anoxia. Palabras clave: Perna viridis, anoxia, cadmio, enzimas antioxidantes, glutatión reducido, lipoperoxidación, metalotioneínas. Anoxia tolerance and antioxidant defences in the green-lipped musselPerna viridis (Linnaeus, 1758) under acute cadmium exposure ABSTRACT. The survival to anoxia was measured from green-lipped mussel Perna viridis after of exposure to 50 µg L -1 cadmium (Cd) during seven days, followed by a recuperation period in the sea during 21 days. The levels of Cd, and oxidative stress biomarkers as metallothioneins (MT), reduced glutathione (GSH) and lipid peroxidation (TBARS), and maximal activities of catalase (CAT), glutathione reductase (GR) and glutathione-S-transferase (GST) were calculated in the digestive gland in experimental organisms. The Cd-exposed organisms showed an anoxia tolerance reduced; however, those organisms recovered in the sea showed a survival time to anoxia major in contrast to control organisms. The Cd-exposed organisms revealed a significant increase of MT and CAT correlated with Cd uptake. The Cd-preexposure and sea-transplanted mussels showed a significant reduction of metal accumulated, whereas the levels of MT was maintain heightened. Both, Cddepurated and non-depurated organisms did not exhibit significant differences among the biomarkers of oxidative stress: TBARS, GSH and enzymes tested. The uptake Cd possibly affected some biochemical mechanisms related to anoxia tolerance, however the high levels of MT in the pre...

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Physiological and cellular responses to copper and mercury in the green mussel Perna viridis (Linnaeus)

Cited by 64 publications

References 28 publications

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Survivorship of juvenile surf clams Donax serra (Bivalvia, Donacidae) exposed to severe hypoxia and hydrogen sulphide

Assessment of Seasonal Variability of Cytochemical Responses to Contaminant Exposure in the Blue Mussel Mytilus edulis (Complex)

Tolerancia a la anoxia y defensas antioxidantes en el mejillon verde Perna viridis (Linneus, 1758) bajo exposicion aguda al cadmio

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