Physiological and genetic characterisation of some new Aphanomyces strains isolated from freshwater crayfish

Royo, Félix; Andersson, Gunnar; Bangyeekhun, Eakaphun; Múzquiz, José Luis; Söderhäll, Kenneth; Cerenius, Lage

doi:10.1016/j.vetmic.2004.09.012

Cited by 37 publications

(39 citation statements)

References 21 publications

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“…Finland is among the last refuges of the Asgenotype, which has been identified as the genotype present in the first invasion of A. astaci to Europe . In most parts of Europe, it has presumably been substituted by other genotypes (PsI, PsII, Pc, Or;Royo et al 2004), after the introductions of alien North American crayfish (Kozubiková et al 2011), which are known carriers of crayfish plague.…”

Section: Introductionmentioning

confidence: 99%

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Makkonen

Jussila²,

Kortet³

et al. 2012

Dis. Aquat. Org.

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Crayfish plague epidemics (caused by Aphanomyces astaci) have been causing population collapses among native European crayfish stocks since the late 1800s. Recent indirect and direct evidence has shown that its virulence has been variable, with native European crayfish even acting as carriers. We tested the differences in A. astaci virulence under experimental conditions using both PsI-and As-genotypes with 3 Finnish noble crayfish Astacus astacus populations. We infected crayfish with adjusted quantities of A. astaci zoospores and monitored the symptoms and mortality of the crayfish. The PsI-genotype isolate caused rapid and total mortality among the tested populations, while the As-genotype isolates expressed more variable virulence. In some cases, mortality among the As-genotype-infected crayfish did not exceed the mortality level of the control group. All of the tested noble crayfish stocks showed lower mortality towards the As-genotype of A. astaci isolated from the River Kemijoki epidemic. We conclude that there are clear differences in virulence between different A. astaci genotypes and also differences in virulence within As-genotypes. Furthermore, we observed clear signs of increased resistance in different populations of noble crayfish towards some of the tested strains belonging to the Asgenotype of A. astaci.

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Section: Introductionmentioning

confidence: 99%

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Makkonen

Jussila²,

Kortet³

et al. 2012

Dis. Aquat. Org.

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“…When tested for their virulence to crayfish species susceptible to A. astaci, these strains failed to cause disease in the European Noble crayfish Astacus astacus and the Australian Cherax destructor (Royo et al 2004). Additionally, we recently isolated an Aphanomyces strain from the signal crayfish Pacifastacus leniusculus.…”

Section: Introductionmentioning

confidence: 99%

Detection of Aphanomyces astaci in North American crayfish by polymerase chain reaction

Oidtmann¹,

Geiger²,

Steinbauer³

et al. 2006

Dis. Aquat. Org.

104

117

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We present a PCR based method to detect Aphanomyces astaci in North American crayfish. Primers were designed to specifically amplify parts of the internal transcribed spacer (ITS) regions and the 5.8 rRNA gene of A. astaci. A single round and a semi-nested assay were tested for their sensitivity and specificity. Specificity of the PCR assays was tested against several closely related Aphanomyces species, other Oomycetes and some non-A. astaci DNA that might be found in or on crayfish. The single round assay was fully specific against all DNA tested. In the semi-nested assay, cross-reaction was seen when the equivalent of 40 000 or more genomic units of A. invadans or A. frigidophilus were entered into the PCR reaction. The lower detection limit of both assays lies around 1 genomic unit of A. astaci. Investigation of various parts of the exoskeleton of 3 North American crayfish species revealed that for O. limosus and P. leniusculus the telson and soft abdominal cuticle yielded a positive PCR reaction most frequently. For the third species, Procambarus clarkii, only 1 individual tested positive, so no conclusion as to preferred infestation site(s) could be drawn.

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“…Similar results were obtained after increasing annealing temperature and reducing the number of cycles ( Figure 1B). Because the species A. frigidophilus and A. repetans, occur in crayfish species (BALLESTEROS et al, 2006;ROYO et al, 2004), and Saprolegnia spp. are ubiquitous with a worldwide and distribution, a great number of false positive is expected when applying the primers used in current crayfish plague PCR-test.…”

Section: Resultsmentioning

confidence: 99%

“…Two new Aphanomyces species were recently isolated from crayfish and crayfish plague cases i.e. Aphanomyces frigidophilus (BALLESTEROS et al, 2006;KITANCHAROEN and HATAI, 1997), and A. repetans (ROYO et al, 2004). The objective of this study was to confront the existing crayfish plague PCR-diagnostic test to recently described Aphanomyces species, and to evaluate the specificity of the primers designed by using real-time PCR.…”

Section: Introductionmentioning

confidence: 99%

Lack of Specificity of the Molecular Diagnostic Method for Identification of Aphanomyces Astaci

Ballesteros

Martı́n

Cerenius

et al. 2007

Bull. Fr. Pêche Piscic.

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Physiological and genetic characterisation of some new Aphanomyces strains isolated from freshwater crayfish

Cited by 37 publications

References 21 publications

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Differing virulence of Aphanomyces astaci isolates and elevated resistance of noble crayfish Astacus astacus against crayfish plague

Detection of Aphanomyces astaci in North American crayfish by polymerase chain reaction

Lack of Specificity of the Molecular Diagnostic Method for Identification of Aphanomyces Astaci

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