Physiological and immune responses of zhikong scallop Chlamys farreri to the acute viral necrobiotic virus infection

Alternation of immune parameters and cellular energy allocation of Chlamys farreri under ammonia-N exposure and Vibrio anguillarum challenge

“…As time progressed, the significantly higher Ec was also recorded in other tissues of scallops at some time-points in comparison with those of control, manifesting that the energy consumption in scallop tissues was enhanced to meet the increased energy demand for immune defense. This was in consistent with previous reports in Ruditapes philippinarum and C. farreri [28,29].…”

Cloning and characterization of a hsp70 gene from Asiatic hard clam Meretrix meretrix which is involved in the immune response against bacterial infection

“…The specific primers hsp-RT-F 0 and hsp-RT-R 0 ( Table 1) were designed near 3 0 end of the sequence of MmeHsc71, and they were used to generate a fragment of 269 bp which was proved to be identity to the sequence of MmeHsc71 by sequencing. PCR was conducted in 25 ml reactions containing 1 ml sample cDNA, 5 pmol of each primer, and Blend Taq-Plus reaction system (TOYOBO), referring to the method of Tang et al [42]. The amplification was carried out on Mastercycler ep realplex 4S (Eppendorf, Germany) machine, with the following profile: 50 C for 2 min, 95 C for 10 min, then 40 cycles of 95 C for 20 s, 57 C for 20 s and 72 C for 1 min.…”

Identification and characterisation of an ostreid herpesvirus-1 microvariant (OsHV-1 µ-var) in Crassostrea gigas (Pacific oysters) in Australia

“…Herpes and herpes-like viruses have been reported in a variety of commercially important oyster species of the genera Crassostrea (Farley et alPecten maximus and Chlamys farreri (Arzul et al 2001a, Tang et al 2010, clams Ruditapes spp. (Arzul et al 2001c) and abalone Haliotis spp.…”

“…Despite the significant impacts of herpesviruses on molluscan aquaculture, ostreid herpesvirus (OsHV) is one of only 3 molluscan herpesviruses (family Malacoherpesviridae) that have been characterised at the molecular level (Davison et al 2009, Savin et al 2010; the others being abalone ganglioneuritis virus from Haliotis spp. (Savin et al 2010) and acute viral necrobiotic virus (AVNV) from the Chinese scallop Chlamys farreri (Tang et al 2010). Phylogenetic comparisons indicate that AVNV and OsHV are variants of the same virus (Renault et al 2012).…”