Physiological and molecular changes in plants grown at low temperatures

Theocharis, Andreas; Clément, Christophe; Barka, Essaïd Ait

doi:10.1007/s00425-012-1641-y

Cited by 584 publications

(384 citation statements)

References 136 publications

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“…Cold acclimation usually involves regulation at transcriptional, post-transcriptional and post-translational levels (Mazzucotelli et al 2008, Theocharis et al 2012. The results of our present spring frost study suggest that PA metabolism is down-regulated in Scots pine needles when the temperature falls below zero.…”

Section: Discussionmentioning

confidence: 52%

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Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Muilu‐Mäkelä¹,

Vuosku²,

Saarinen³

et al. 2017

iForest

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(1) Polyamines (PA) are ubiquitous polycations known to be involved in several phases of plant development as well as in tolerance to abiotic stresses. Phenols are complex secondary metabolites produced via the phenylpropanoid pathway that contain, e.g., cell wall compounds and antioxidants. Phenols are known to enhance chilling tolerance of plants. PA and phenolic pathways are connected via conjugation. In boreal coniferous forests spring frost has been considered to have severe effects on the survival of tree seedlings. Such effects are likely to increase in the future. The present study focuses on the role of PA and phenylpropanoid syntheses in the coping strategies of Scots pine exposed to cold temperatures during the vulnerable early seedling phase in late spring and early summer. We found that spring frost affects the expression of genes regulating PA metabolism and phenylpropanoid synthesis differently in above and below ground parts of the seedlings, whereas PA or phenol contents in tissues were not affected. The results suggest that Scots pine seedlings may not have time to develop metabolite level responses during a short period of freezing stress and, therefore, the originally different PA levels, especially in roots, may influence the tolerance of Scots pine seedlings to spring frost.

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Section: Discussionmentioning

confidence: 52%

“…The primary site to sense freezing temperatures are cell membranes, where a cascade of cellular processes is initiated (Theocharis et al 2012). Sugars, amino acids, organic acids, PAs and lipids are all actively regulated in response to stress in plant cells (Krasensky & Jonak 2012).…”

Section: Introductionmentioning

confidence: 99%

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Muilu‐Mäkelä¹,

Vuosku²,

Saarinen³

et al. 2017

iForest

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“…Cold stress increases the cytosolic Ca 2+ level, and this is then sensed by Ca 2+ sensor proteins such as calmodulin (CaM) and calcineurin B-like proteins (CBLs) [36,37]. The increased levels of Ca 2+ in plant cells affect the expression level of CBF and COR genes in the cold signaling pathway [7,38]. In this study, 332 unigenes were annotated as CaMs and CBLs, and included 14 DEGs.…”

Section: Discussionmentioning

confidence: 99%

De Novo Transcriptome Sequencing in Passiflora edulis Sims to Identify Genes and Signaling Pathways Involved in Cold Tolerance

Liu

Chen

et al. 2017

Forests

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Abstract:The passion fruit (Passiflora edulis Sims), also known as the purple granadilla, is widely cultivated as the new darling of the fruit market throughout southern China. This exotic and perennial climber is adapted to warm and humid climates, and thus is generally intolerant of cold. There is limited information about gene regulation and signaling pathways related to the cold stress response in this species. In this study, two transcriptome libraries (KEDU_AP vs. GX_AP) were constructed from the aerial parts of cold-tolerant and cold-susceptible varieties of P. edulis, respectively. Overall, 126,284,018 clean reads were obtained, and 86,880 unigenes with a mean size of 1449 bp were assembled. Of these, there were 64,067 (73.74%) unigenes with significant similarity to publicly available plant protein sequences. Expression profiles were generated, and 3045 genes were found to be significantly differentially expressed between the KEDU_AP and GX_AP libraries, including 1075 (35.3%) up-regulated and 1970 (64.7%) down-regulated. These included 36 genes in enriched pathways of plant hormone signal transduction, and 56 genes encoding putative transcription factors. Six genes involved in the ICE1-CBF-COR pathway were induced in the cold-tolerant variety, and their expression levels were further verified using quantitative real-time PCR. This report is the first to identify genes and signaling pathways involved in cold tolerance using high-throughput transcriptome sequencing in P. edulis. These findings may provide useful insights into the molecular mechanisms regulating cold tolerance and genetic breeding in Passiflora spp.

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“…In order to combat low temperatures, plants undergo a series of physiological, biochemical, and gene expression changes when they encounter cold stress (Thomashow, 1999;Theocharis et al, 2012). We found that CsICE1 could be induced by exogenous ABA treatment, which indicates that CsICE1 is possibly involved in the ABA signaling pathway.…”

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confidence: 80%

Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis

Ding¹,

Li²,

Hong³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. CsICE1 is thought to be involved in hardiness resistance of tea plants. Using seedling cuttings of biennial Wuniuzao in this study, the pattern of CsICE1 expression under cold temperature (4°, -5°C), drought [20% polyethylene glycol 6000 (PEG-6000)], and plant hormone [200 mg/L abscisic acid (ABA), 1 mg/L brassinolide (BR)] treatment was studied by real-time quantitative PCR. Additionally, stress resistance, such as the freezing resistance of CsICE1, was studied using Arabidopsis lines transformed with sense or anti-sense CsICE1 via Agrobacterium tumefaciens infection. Our results showed that CsICE1 mRNA could be induced under -5°C, PEG, ABA, or BR treatment, although the pattern of expression differed for all treatments. Compared to wild type (WT) and anti-sense ICE1 transgenic lines, sense lines displayed higher relative germination rates under salt and drought stress. After freezing treatment, the sense transgenic lines overexpressing CsICE1 showed a higher survival rate, increased levels of proline, and decreased levels of malonaldehyde. Conversely, compared with WT, anti-sense ICE1 transgenic lines had lower proline levels and higher malonaldehyde levels under freezing conditions. Our study 11260 Z.T. Ding et al. ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 11259-11270 (2015) indicates that CsICE1 is an important anti-freezing gene and that overexpression of CsICE1 can improve cold resistance and enhance salt and drought tolerance of transgenic lines.

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Physiological and molecular changes in plants grown at low temperatures

Cited by 584 publications

References 136 publications

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

Coping with spring frost-effects on polyamine metabolism of Scots pine seedlings

De Novo Transcriptome Sequencing in Passiflora edulis Sims to Identify Genes and Signaling Pathways Involved in Cold Tolerance

Pattern of CsICE1 expression under cold or drought treatment and functional verification through analysis of transgenic Arabidopsis

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