Physiological and Molecular Insights to Drought Responsiveness in Erianthus spp.

Augustine, Sruthy Maria; Syamaladevi, Divya P.; Premachandran, M. N.; Ravichandran, V.

doi:10.1007/s12355-014-0312-7

Cited by 24 publications

(16 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We discovered that overcoming the trade‐off between growth and abiotic stress tolerance in plants is possible using a gene‐stacking approach with genes whose expression is repressed under drought stress conditions in Arabidopsis. On the other hand, Erianthus was reported to show high levels of both growth ability and drought stress tolerance even without the gene‐stacking approach (Augustine et al ., ). However, in this plant as well as in Arabidopsis, DREB expression was increased under drought stress conditions, and expansin gene expression was associated with growth retardation under the stress conditions (Augustine et al ., ).…”

Section: Discussionmentioning

confidence: 97%

“…However, the trade‐off between plant growth and drought stress tolerance greatly varies between plant species. For example, Erianthus, which is a species closely related to sugarcane, shows both high levels of growth ability and drought stress tolerance (Augustine et al ., ). Therefore, overcoming the inherent trade‐off between growth and drought stress tolerance in plants is possible.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

A gene‐stacking approach to overcome the trade‐off between drought stress tolerance and growth in Arabidopsis

et al. 2018

View full text Add to dashboard Cite

The molecular breeding of drought stress-tolerant crops is imperative for stable food and biomass production. However, a trade-off exists between plant growth and drought stress tolerance. Many drought stress-tolerant plants overexpressing stress-inducible genes, such as DEHYDRATION-RESPONSIVE ELE-MENT-BINDING PROTEIN 1A (DREB1A), show severe growth retardation. Here, we demonstrate that the growth of DREB1A-overexpressing Arabidopsis plants could be improved by co-expressing growth-enhancing genes whose expression is repressed under drought stress conditions. We used Arabidopsis GA REQUIRING 5 (GA5), which encodes a rate-limiting gibberellin biosynthetic enzyme, and PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), which encodes a transcription factor regulating cell growth in response to light and temperature, for growth improvement. We observed an enhanced biomass and floral induction in the GA5 DREB1A and PIF4 DREB1A double overexpressors compared with those in the DREB1A overexpressors. Although the GA5 DREB1A double overexpressors continued to show high levels of drought stress tolerance, the PIF4 DREB1A double overexpressors showed lower levels of stress tolerance than the DREB1A overexpressors due to repressed expression of DREB1A. A multiomics analysis of the GA5 DREB1A double overexpressors showed that the co-expression of GA5 and DREB1A additively affected primary metabolism, gene expression and plant hormone profiles in the plants. These multidirectional analyses indicate that the inherent trade-off between growth and drought stress tolerance in plants can be overcome by appropriate gene-stacking approaches. Our study provides a basis for using genetic modification to improve the growth of drought stress-tolerant plants for the stable production of food and biomass.Keywords: drought and cold stress tolerance, dehydration-responsive element-binding protein 1A (DREB1A), GA REQUIRING 5 (GA5), PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), enhanced biomass and floral induction, Arabidopsis. Figure 7. Endogenous gibberellin (GA) levels in GA5 DREB1A double overexpressors. Endogenous GA levels in 2-week-old transgenic plants. GAs are shown according their order in the biosynthesis pathway. ND indicates not detected. The error bars show the SD of more than three samples. The letters indicate significant differences among the samples (P < 0.05 according to Tukey's multiple range test). Solid and dashed lines indicate GA biosynthesis and deactivation pathways, respectively. GGDP, geranylgeranyl diphosphate; GA2ox, GA 2-oxidase; GA20ox, GA 20-oxidase; GA3ox, GA 3-oxidase. Co-expression of DREB1 and growth enhancers 251 Plant hormone analysisPlant hormones were extracted from whole plants grown on the agar medium for 2 weeks. The extraction, purification and

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

A gene‐stacking approach to overcome the trade‐off between drought stress tolerance and growth in Arabidopsis

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Heikkila et al ( 1984 ) reported a relationship between ABA, drought stress, and the expression of Hsp70 genes. In Erianthus arundinaceus, a highly drought-tolerant plant (Augustine et al 2014 ), Hsp70 was expressed sevenfold higher under droughtstressed condition compared to the irrigated plants (Augustine et al 2015 ). Up-regulation of some of the Hsps is only during the photosynthetic acclimation, e.g., Hsc70-1 and Hsc70-3 (Vasquez-Robinet et al 2008 ).…”

Section: Phenomena Of Induction Of Hsps In Plants During Drought Stressmentioning

confidence: 99%

“…Kuznetsov et al ( 1999 ) reported that adaptation of cotton plant to drought stress was along with resistance to high temperature. The induction of Hsp was reported in both laboratory and fi eld grown plants when subjected to drought stress (Prasad 1997 ;Augustine et al 2014 ). Hu et al ( 2009 ) analyzed the expression profi ling of heat-stressed rice and compared with the data for drought, cold, and salt stresses.…”

Section: Phenomena Of Induction Of Hsps In Plants During Drought Stressmentioning

confidence: 99%

Function of Heat-Shock Proteins in Drought Tolerance Regulation of Plants

Augustine

2016

Drought Stress Tolerance in Plants, Vol 1

Self Cite

View full text Add to dashboard Cite

“…and have been reported as high as 59 dry Mg ha −1 year −1 (Jackson & Henry, ; Mislevy, Martin, Adjei, & Miller, ; Palmer et al, ). Tripidium arundinaceum is characterized by dense vegetative culms, drought tolerance, disease and pest resistance, and low input requirements (Amalraj, Rakkiyappan, & Rema Devi, ; Augustine, Syamaladevi, Premachandran, Ravichandran, & Subramonian, ; Berding & Roach, ; Cai et al, ). Tripidium arundinaceum is also known for its tillering ability and ratooning performance, making it desirable for introgression with energycane, sugarcane, and bioenergy grass improvement programs (Amalraj et al, ; Berding & Roach, ; Cai et al, ; Sugimoto, ).…”

Section: Introductionmentioning

confidence: 99%

Biomass yields, cytogenetics, fertility, and compositional analyses of novel bioenergy grass hybrids (Tripidium spp.)

et al. 2020

View full text Add to dashboard Cite

High biomass yields have been documented for Tripidium spp. (Erianthus spp., Saccharum spp.), but targeted breeding for bioenergy applications has been limited. Advanced, interspecific hybrids between Tripidium ravennae and T. arundinaceum were planted in replicated field plots in 2016. Comparative feedstock evaluations examined biomass yields, cytogenetics, plant fertility, and compositional analyses relative to Miscanthus × giganteus. Dry biomass yields varied as a function of year and accession and increased each year ranging from 3.4 to 10.6, 8.6 to 37.3, and 23.7 to 60.6 Mg/ha for Tripidium hybrids compared to 2.3, 16.2 and 27.9 Mg/ha for M. × giganteus in 2016, 2017, and 2018, respectively. Cytology and cytometry confirmed that Tripidium hybrids were tetraploid with 2n = 4x = 40 (2C genome size = 5.06 pg) and intermediate between T. ravennae with 2n = 2x = 20 (2C genome size = 2.55 pg) and T. arundinaceum with 2n = 6x = 60 (2C genome size = 7.61 pg). Plant fertility characteristics varied considerably with some accessions producing no viable seeds or fewer than that observed for M. × giganteus. Accessions varied significantly for flowering culm number and height and dates of peak anthesis ranging from 14 September to 2 October. Variations in yield and compositional analyses contributed to variations in theoretical ethanol yields ranging from 10,181 to 27,546 L/ ha for Tripidium accessions compared to 13,095 L/ha for M. × giganteus. Relative feed value (RFV) indices for winter-harvested Tripidium accessions varied from 52.8 to 60.0 compared to M. × giganteus with 45.4. RFV for summer-harvested Tripidium accessions varied from 71.6 to 80.5 compared to M. × giganteus with 61.0. These initial findings for Tripidium hybrids, including high biomass yields, cold hardiness, and desirable traits for multiple markets (e.g., forage, bioenergy, bioproducts), are promising and warrant further development of Tripidium as a temperate bioenergy feedstock. K E Y W O R D S bioenergy, bioprocessing, Erianthus, fertility, forage, lignocellulose, Miscanthus, plant breeding, polyploidy, reproductive biology, Saccharum, sterility, Tripidium [Correction added on 13 April 2020 after first online publication: the genome values have been updated in the Abstract and Results sections in this version.] 362 | MAREN Et Al.Note: Superscript letters indicate statistically significant value p ≤ .05. 1 2C DNA values represent the mean value of three to six samples conducted for each taxon.2 Taxa followed by an asterisk were confirmed with cytology. 3 Values represent the mean ± SEM. Mean separation within columns by Fisher's least significant difference at p ≤ .05. 4 Calculated as (seed set × seed germination)/100. 5 Flowering was not observed within the growing season at the Fletcher, NC evaluation site.

show abstract

Physiological and Molecular Insights to Drought Responsiveness in Erianthus spp.

Cited by 24 publications

References 28 publications

A gene‐stacking approach to overcome the trade‐off between drought stress tolerance and growth in Arabidopsis

A gene‐stacking approach to overcome the trade‐off between drought stress tolerance and growth in Arabidopsis

Function of Heat-Shock Proteins in Drought Tolerance Regulation of Plants

Biomass yields, cytogenetics, fertility, and compositional analyses of novel bioenergy grass hybrids (Tripidium spp.)

Contact Info

Product

Resources

About