Physiological and Pathological Cell Deaths in the Reproductive Organs.

Sakamaki, Kazuhiro

doi:10.1247/csf.28.31

Cited by 13 publications

(12 citation statements)

References 144 publications

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“…Spontaneous germ cell death is commonly seen during mammalian spermatogenesis [19]. Germ cells die at several differentiation steps, including fetal, pubertal, as well as in the adult period.…”

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confidence: 99%

Developmental Expression of p63 in the Mouse Testis

Nakamuta

Kobayashi

2004

The Journal of Veterinary Medical Science

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ABSTRACT. p63 is a member of the p53 gene family and have structural similarities with p53. p63 encodes for multiple isotypes either with N-terminal transactivation domain (TAp63) or without it ( ∆Np63). In the mammalian testis, it has been shown that p53 plays important roles in the regulation of germ cell apoptosis and meiosis. However, little is known for the physiological function of p63 in the mammalian spermatogenesis. To investigate the potential roles of p63 in the developing mouse testis, we examined the expression pattern of p63 in the mouse testis from birth to adulthood. In addition to the TAp63 mRNA which was continuously expressed in the developing testis, transcripts encoding ∆Np63 was detected at specific stages of testicular development by RT-PCR, from postnatal day 1 to day 7 and from 3 weeks to 4 weeks after birth. Western blot analysis of whole testis lysates with anti-p63 antibody revealed an approximately 68 kD band throughout development and a less abundant protein at 60 kD in the earlier period of postnatal development. Immunopositive reactions for p63 were observed as early as 10 days after birth and p63 protein was localized to the nuclei of spermatocytes and round spermatids. These findings strongly suggest that p63 might be involved in the regulation of proliferation and differentiation of spermatogenic cells in the developing mouse testis.

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confidence: 99%

Developmental Expression of p63 in the Mouse Testis

Nakamuta

Kobayashi

2004

The Journal of Veterinary Medical Science

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“…The stringently regulated apoptosis, involving in cell proliferation, differentiation, self-renewal and removal of abnormal cells, plays a crucial role in spermatogenesis at physiological normal conditions as well as after exposure to the pathological stimuli and damage including radiation (Print and Loveland, 2000;Pentikainen et al, 2003;Sakamaki, 2003). Gonocytes proliferate actively around E15 and distribute among all the phases of cell cycle.…”

Section: Discussionmentioning

confidence: 99%

“…These results maybe consequently had certain possible acute and late influence on apoptosis induction in the gonocytes and the development of testes. Cell death regulators, such as Bcl-2 family members, caspases, Fas, p53 and p21 are involved in apoptosis after irradiations (Moreno et al, 2001b(Moreno et al, , 2002Sakamaki, 2003). Analysis of gene expressions would provide a great insight into the mechanisms, especially the time course investigation, which can provide a distinct and more definitive view into the genes implicated in the maturation, maintenance, and function of the testis and the integrated process of spermatogenesis (Shima et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

Effects of prenatal irradiation with accelerated heavy-ion beams on postnatal development in rats: III. Testicular development and breeding activity

Wang¹,

Murakami²,

Eguchi-Kasai³

et al. 2007

Advances in Space Research

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“…20 One of the most important and widely known regulatory system of apoptosis is Bcl2 family which composed of anti and pro-apoptotic proteins. These proteins regulate apoptosis by controlling the release of cytochrome c and other mitochondrial changes.…”

Section: Anan Hh Et Al Int J Sci Rep 2017 Feb;3(2):39-48mentioning

confidence: 99%

Histological and immunohistochemical study of cyclophosphamide effect on adult rat testis

et al. 2017

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Background: Nowadays, cyclophosphamide is widely used as anticancer and immunosuppressive agent in various drug regimens in many diseases and in young and old age. The aim of this research is to study the possible histological changes that may occur in the testes of adult male albino rats as a result of chronic exposure to cyclophosphamide and the prognosis of this effect.Methods: Thirty healthy adult male albino rats were used in this study. They were equally divided into three groups; a control, an experimental and a withdrawal groups. The Animals of the experimental group were treated with daily dose of 5 mg/ kg cyclophosphamide orally for successive 28 days. Animals of the withdrawal group were left without treatment and sacrificed after 28 days from the last dose of cyclophosphamide. At the time of sacrifice, all animals were anesthetized by ether inhalation and their testes were dissected out carefully and processed for light and electron microscope examinations. Results: Testes of the cyclophosphamide treated group revealed presence of many distorted shrunken seminiferous tubules which appeared with marked reduction in the thickness of the epithelium and wide lumina. Many germ cells with deeply stained nuclei, giant cells in mitosis and intracellular vacuoles were observed. Cross sections in mid pieces of sperms showed marked affection of axoneme, fibrous sheath and mitochondrial sheath. The cytoplasm of the Leydig cells contained mitochondria, dilated SER, Golgi cisternae and RER. Testes of the withdrawal group showed that the seminiferous tubules still had reduced height of their epithelium with wide intercellular spaces. Abnormal stratification and destructed germinal epithelium were evident with desquamated germ cells. Cross sections of mid pieces of the sperms showed distorted axoneme and swollen mitochondrial sheath. The cytoplasm of leydig cells contained many electron dense granules, RER, many dilated SER and mitochondria.Conclusions: Chronic cyclophosphamide treatment not only produced serious histological changes of the testis but also in its serological parameter. These changes persisted after cessation of cyclophosphamide administration which indicates the cumulative irreversible toxic effect of cyclophosphamide. So, it is advisable to avoid the usage of cyclophosphamide as possible especially in young patients.

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Physiological and Pathological Cell Deaths in the Reproductive Organs.

Cited by 13 publications

References 144 publications

Developmental Expression of p63 in the Mouse Testis

Developmental Expression of p63 in the Mouse Testis

Effects of prenatal irradiation with accelerated heavy-ion beams on postnatal development in rats: III. Testicular development and breeding activity

Histological and immunohistochemical study of cyclophosphamide effect on adult rat testis

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