Physiological and proteomic analysis in two wild tomato lines under waterlogging and high temperature stress

Abstract

Tomato yields are reduced under waterlogging and high temperature stress condition. Ascorbic acid (ASA) was shown to be involved in tolerance to waterlogging and heat stresses in tomato. Among 44 wild tomato lines treated with waterlogging at 38°C, L6138 (Solanum peruvianum) showed highest ASA, shoot growth, chlorophyll content and chlorophyll fluorescence in comparison to other tomato lines. Further leaf proteins in L6138 and L0994 under waterlogging at 38°C for 72 h were analyzed by two-dimensional protein fractionation system. Different protein peaks were analyzed by comparative proteomic analysis and database searching. Fifty protein peaks expressed in response to stress treatment were identified, among which 27 proteins from L0994 and 17 proteins from L6138 were successfully sequenced. Differentially proteins expressed have major functions in protein structure maintenance, metabolism, secretion, translation, biosynthesis, signal transduction, degradation, and photosynthesis under stress. ASA might be played a role in tolerance to waterlogging and heat stress by maintaining RNA transcription, protein structure, and metabolism. In this study, we utilized a physiological and proteomic approach to discover the changes in protein expression profiles of tomatoes in response to heat and flood stresses.

Selection of Tomato and Cucumber Accessions for Waterlogging Sensitivity through Morpho-Physiological Assessment at an Early Vegetative Stage

“…The decrease in Fv/Fm was noted in more sensitive tomato and cucumber plants in our experiments. In agreement with our results, a decrease in Fv/Fm after waterlogging was also observed in rice [54,57], cucumber [56,58], tomato [59,60], wild tomato (Solanum habrochaites S.Knapp & D.M.Spooner) [61], Arabidopsis (Arabidopsis thaliana L.) [62], cotton [39], and pepper (Capsicum annuum L.) plants [63]. Barik et al [57] examined the reaction of tolerant and susceptible varieties of rice to submergence and observed that the latter exhibited a greater reduction in Fv/Fm parameters in comparison to tolerant varieties.…”

Proteomic Analysis Reveals the Positive Effect of Exogenous Spermidine in Tomato Seedlings' Response to High-Temperature Stress

“…High-temperature stress represents one of the most frequent abiotic stresses worldwide, inducing several physiological and biochemical processes in cells, and limiting the growth and productivity of plants (Bita and Gerats, 2013 ). Plants respond to high temperature by reprogramming their proteome, metabolome and transcriptome to establish a new steady-state balance of metabolic processes (Kosová et al, 2011 ; Lin H. H. et al, 2015 ; Sruthi et al, 2016 ).…”

Different ways to die in a changing world: Consequences of climate change for tree species performance and survival through an ecophysiological perspective

“…Similarly, high temperature combined with flooding can further compromise the performance of plant species through additional damages to the photosynthetic process, changes in root respiration, and also by compromising the synthesis of structural components (e.g., cell wall). However, it is important to note that only a few studies have addressed the links between these two stresses in great detail (Chen et al, , ; Donovan, Stumpff, & McLeod, ; Lin, Lin, Syu, Tang, & Lo, ), and thus, our knowledge about this topic, especially on wood species, is rather fragmented.…”