Physiological and proteomic characterization of manganese sensitivity and tolerance in rice (Oryza sativa) in comparison with barley (Hordeum vulgare)

Führs, Hendrik; Behrens, Christof; Gallien, Sébastien; Heintz, Dimitri; Dorsselaer, Alain Van; Braun, Hans‐Peter; Horst, Walter J.

doi:10.1093/aob/mcq046

Cited by 81 publications

(61 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…If the OsYSL2 and OsYSL6 transporters are unable to transport Mn:malate complexes to the leaf symplast, then the accumulation of Mn in the apoplast could trigger stress symptoms, even when the total concentrations of Mn in the leaves are not normally considered harmful to rice. This hypothesis is consistent with the role of the leaf apoplast in Mn toxicity convincingly proposed by others (Führs et al, 2010) and with phenotypes described in ysl6 mutants in rice (see below). The model presented in Supplemental Figure S10 attempts to summarize the proposed changes in the OX lines.…”

Section: Symptoms Of Mn Toxicity Are Not Correlated With Total Mn Consupporting

confidence: 92%

“…Other studies have reported a similar lack of correlation between tissue Mn concentration and stress symptoms (Führs et al, 2010(Führs et al, , 2012Sasaki et al, 2011), and several explanations could account for this. For instance, mineral concentrations in leaves can vary due to plant age, nutrition supply, and soil chemistry.…”

Section: Symptoms Of Mn Toxicity Are Not Correlated With Total Mn Conmentioning

confidence: 58%

“…3). These general symptoms have been associated with many disorders, including oxidative stress, pathogens, and nutrient deficiencies and nutrient toxicities, including Mn and B (Marschner, 1995;Führs et al, 2010). Pathogen infection was discounted here, since symptoms occurred in all growth environments (hydroponics, soil, glasshouses, and cabinets) and only on the OX lines.…”

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

“…Indeed, rice can accumulate 1% (w/w) Mn with minimal signs of stress (Vlamis and Williams, 1964). Mn toxicity can likely be triggered at different cellular sites, but the apoplast of leaves appears to be important (Führs et al, 2009(Führs et al, , 2010. Mn toxicity induces brown spots and necrotic lesions on the leaves, which are caused, in part, by the oxidization of Mn and phenolic compounds in the apoplast, which damage proteins and lipids (Fecht-Christoffers et al, 2003.…”

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

“…The statistical test used to compare these ratios is described in "Materials and Methods." apoplast or the sequestration of Mn into subcellular compartments such as the vacuole, Golgi, and endoplasmic reticulum and the induction of protective proteins in the chloroplast (Lidon and Teixeira, 2000;Delhaize et al, 2007a;Führs et al, 2010;Fernando and Lynch, 2015).…”

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

See 4 more Smart Citations

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

et al. 2017

View full text Add to dashboard Cite

ORCID IDs: 0000-0003-1555-4754 (J.L.); 0000-0003-3009-7854 (M.Z.); 0000-0002-1376-9543 (P.R.R.).Aluminum-activated malate transporters (ALMTs) form a family of anion channels in plants, but little is known about most of its members. This study examined the function of OsALMT4 from rice (Oryza sativa). We show that OsALMT4 is expressed in roots and shoots and that the OsALMT4 protein localizes to the plasma membrane. Transgenic rice lines overexpressing (OX) OsALMT4 released malate from the roots constitutively and had 2-fold higher malate concentrations in the xylem sap than nulls, indicating greater concentrations of malate in the apoplast. OX lines developed brown necrotic spots on the leaves that did not appear on nulls. These symptoms were not associated with altered concentrations of any mineral element in the leaves, although the OX lines had higher concentrations of Mn and B in their grain compared with nulls. While total leaf Mn concentrations were not different between the OX and null lines, Mn concentrations in the apoplast were greater in the OX plants. The OX lines also displayed increased expression of Mn transporters and were more sensitive to Mn toxicity than null plants. We showed that the growth of wild-type rice was unaffected by 100 mM Mn in hydroponics but, when combined with 1 mM malate, this concentration inhibited growth. We conclude that increasing OsALMT4 expression affected malate efflux and compartmentation within the tissues, which increased Mn concentrations in the apoplast of leaves and induced the toxicity symptoms. This study reveals new links between malate transport and mineral nutrition.

show abstract

Section: Symptoms Of Mn Toxicity Are Not Correlated With Total Mn Consupporting

confidence: 92%

Section: Symptoms Of Mn Toxicity Are Not Correlated With Total Mn Conmentioning

confidence: 58%

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

Section: Discussion Malate Efflux From Cells Disrupts Mn Nutrition Inmentioning

confidence: 99%

See 3 more Smart Citations

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

et al. 2017

View full text Add to dashboard Cite

show abstract

Manganese Toxicity and Tolerance in Photosynthetic Organisms and Breeding Strategy for Improving Manganese Tolerance in Crop Plants

Takagi

2023

Heavy Metal Toxicity and Tolerance in Plants

View full text Add to dashboard Cite

Populus cathayana males are less affected than females by excess manganese: Comparative proteomic and physiological analyses

et al. 2013

View full text Add to dashboard Cite

The comprehension of sexually different responses in dioecious plants to excess manganese (Mn) stress requires molecular explanation. Physiological and proteomic changes in leaves of Populus cathayana males and females were analyzed after 4 wk of exposure to Mn stress. Under excess Mn conditions, shoot height and photosynthesis decreased more in females than in males. Females also showed severe browning and subcellular damage, higher Mn2+ absorption, and different antioxidant enzyme activities compared with males. There were ten differently regulated protein spots induced by excess Mn stress. They were mainly related to photosynthesis, ROS cleaning, and cell signaling associated to ROS, plant cell death, heat shock, cell defense and rescue, and gene expression and regulation. Variation in protein expression between the sexes clearly showed that males have evolved more efficient photosynthesis capacity, more stable gene expression and regulation, and better cell defense and rescue to prevent further injury under excess Mn stress.

show abstract

Physiological and proteomic characterization of manganese sensitivity and tolerance in rice (Oryza sativa) in comparison with barley (Hordeum vulgare)

Cited by 81 publications

References 57 publications

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

Altered Expression of a Malate-Permeable Anion Channel, OsALMT4, Disrupts Mineral Nutrition

Manganese Toxicity and Tolerance in Photosynthetic Organisms and Breeding Strategy for Improving Manganese Tolerance in Crop Plants

Populus cathayana males are less affected than females by excess manganese: Comparative proteomic and physiological analyses

Contact Info

Product

Resources

About