Physiological and transcriptomic responses of Lanzhou Lily (Lilium davidii, var. unicolor) to cold stress

Tian, Xuehui; Xie, Jianming; Yu, Jiangnan

doi:10.1371/journal.pone.0227921

Cited by 37 publications

(17 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Bulbs of the lily Lilium davidii var. unicolor are one of the main traditional Chinese medicines for several centuries, and its owers also has ornamental value [1,2]. In recent years, more and more studies focused on bulbs of Lilium davidii var.…”

Section: Introductionmentioning

confidence: 99%

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

Wang¹,

Hou²,

Qi³

et al. 2021

Preprint

View full text Add to dashboard Cite

Background: The high quality, high yield and purity RNA samples are essential for subsequent molecular experiments such as RT-PCR, qPCR and RNA-seq. However, harvest high quality RNA samples from different tissues in Lilium davidii var. unicolor is a great challenge due to its polysaccharides, polyphenols and other secondary metabolites. In this study, three RNA extraction methods, namely modified TRIzol method, Kit and CTAB methods were reported to obtain the total RNA from Lilium davidii var. unicolor, and the efficient RNA extraction protocol (modified TRIzol method) was described. Results: A Nano drop spectrophotometer and 1% gel electrophoresis were used to detect the RNA quality and integrity. Compared with Kit and CTAB methods, the higher RNA concentrations from different tissues were obtained and the A260/280 ratios of RNA samples were ranged from1.97 to 2.27 when using the modified TRIzol method. None of intact RNA bands were gained from the modified CTAB method, indicating that the RNA samples isolation were degraded. As a result of the modified TRIzol method, the RNA samples in the different tissues from Lilium presented clear 28S and 18S bands.Conclusions: Here, modified TRIzol method is an easy, efficient, and low-cost method for RNA isolation from Lilium davidii var. unicolor. Thus, modified TRIzol method is sufficient to gain eligible RNA to support further molecular experiments in Lilium davidii var. unicolor.

show abstract

Section: Introductionmentioning

confidence: 99%

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

Wang¹,

Hou²,

Qi³

et al. 2021

Preprint

View full text Add to dashboard Cite

show abstract

“…Lanzhou lily is famous for its large, white and delicate taste bulbs, which is rich in minerals, trace elements, amino acids and other nutrients [ 1 ]. It is mainly grown in Gansu Province, China and is a very important agricultural plant with high economic value [ 2 , 3 ]. As a traditional medicinal plant and popular edible vegetable bulb, Lanzhou lily also has the function of clearing away heat and removing toxic, nourishing the lungs, anting cancer and enhancing human immunity [ 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Changes of starch and sucrose content and related gene expression during the growth and development of Lanzhou lily bulb

Huang

Wang

et al. 2022

PLoS ONE

View full text Add to dashboard Cite

As the main forms of carbohydrates, starch and sucrose play a vital role in the balance and coordination of various carbohydrates. Lanzhou lily is the most popular edible lily in China, mainly distributed in the central region of Gansu. To clarify the relationship between carbohydrate metabolism and bulb development of Lanzhou lily, so as to provide a basis for the promotion of the growth and development in Lanzhou lily and its important economic value, we studied lily bulbs in the squaring stage, flowering stage, half withering stage and withering stage. The plant height, fresh weight of mother and daughter bulbs continued to increase during the whole growth period and fresh weight of stem and leaf began to decrease in the half withering stage. The content of starch, sucrose and total soluble sugar in the lily mother bulb accumulated mostly in the flowering, withering and half withering stages, respectively. Starch, sucrose and total soluble sugar accumulated in the daughter bulb with the highest concentration during the withering stage. In the transcription level, sucrose synthase (SuSy1) and sucrose invertase (INV2) expressed the highest in squaring stage, and the expression was significantly higher in the mother bulb than in the daughter bulb. In flowering stage, the expression levels of soluble starch synthase (SSS1), starch-branching enzyme (SBE) and adenosine diphosphate-glucose pyrophosphorylase (AGP1) genes were higher in the mother bulb than in the daughter bulb. Altogether, our results indicate that starch and sucrose are important for the bulb growth and development of Lanzhou lily.

show abstract

“…Besides, the bulbs of Lilium d avidii var. unicolor are rich in nutrients, such as vitamins, proteins, amino acids, dietary fiber, and starch, as well as a variety of bioactive substances, including olysaccharides, alkaloids, and saponins (Tian et al, 2020). There are various approaches that can achieve the reproduction of the Lilium genus, including scale cuttings, bulblets or bulblets on stems, bulbils, seed reproduction, and tissue culture (De Klerk, 2012).…”

Section: Introductionmentioning

confidence: 99%

Methane‐rich water induces bulblet formation of scale cuttings in Lilium davidii var. unicolor by regulating the signal transduction of phytohormones and their levels

Hou

Wang

et al. 2021

Physiologia Plantarum

View full text Add to dashboard Cite

Previous studies have shown that methane (CH 4 ) has promoting roles in the adventitious root (AR) and lateral root (LR) formation in plants. However, whether CH 4 could trigger the bulblet formation in scale cutting of Lilium davidii var. unicolor has not been elucidated. To gain insight into the effect of CH 4 on the bulblet formation, different concentrations (1, 10, 50, and 100%) of methane-rich water (MRW) and distilled water were applied to treat the scale cuttings of Lilium. We observed that treatment with 100% MRW obviously induced the bulblet formation in scale cuttings.To explore the mechanism of CH 4 -induced bulblet formation, the transcriptome of scales was analyzed. A total of 2078 differentially expressed genes (DEGs) were identified. The DEGs were classified into different metabolic pathways, especially phenylpropanoid biosynthesis, starch and sucrose metabolism, and plant signal transduction. Of these, approximately 38 candidate DEGs involved in the plant signal transduction were further studied. In addition, the expression of AP2-ERF/ERF, WRKY, GRAS, ARF, and NAC transcription factors (TFs) was changed by MRW treatment, suggesting their potential involvement in bulblet formation. As for hormones, exogenous IAA, GA, and ABA could induce the bulblet formation. Additional experiments suggested that MRW could increase the endogenous IAA, GA, and JA levels, but decrease the levels of ABA during bulblet formation, which showed that higher IAA, GA, JA levels and lower ABA content might facilitate bulblet formation. In addition, the levels of endogenous hormones were consistent with the expression level of genes involved in phytohormone signal transduction. Overall, this study has revealed that CH 4 might improve the bulblet formation of cutting scales in Lilium by regulating the expression of genes related to phytohormone signal transduction and TFs, as well as by changing the endogenous hormone levels. | INTRODUCTIONLilies (Lilium spp.) are one of the most important bulbous plants in the floriculture industry, with high ornamental, economic, medicinal, and edible values (Li et al., 2014). Lilium davidii var. unicolor, a mutation of L. davidii Duchartre and one of the best edible Lilium in China, is renowned for its extremely special sweet taste (Xu et al., 2009). The flamboyant color of its flowers provides its ornamental value. Besides, the bulbs of Lilium davidii var. unicolor are rich in nutrients, such as vitamins, proteins, amino acids, dietary fiber, and starch, as well as a variety of bioactive substances, including olysaccharides, alkaloids, and saponins (Tian et al., 2020). There are various approaches that

show abstract

Physiological and transcriptomic responses of Lanzhou Lily (Lilium davidii, var. unicolor) to cold stress

Cited by 37 publications

References 66 publications

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

An optimized method to obtain high-quality RNA from different tissues in Lilium davidii var. unicolor

Changes of starch and sucrose content and related gene expression during the growth and development of Lanzhou lily bulb

Methane‐rich water induces bulblet formation of scale cuttings in Lilium davidii var. unicolor by regulating the signal transduction of phytohormones and their levels

Contact Info

Product

Resources

About