Physiological characteristics and metabolomics reveal the tolerance mechanism to low nitrogen in <i>Glycine soja</i> leaves

Zhao, Mingli; Guo, Rui; Li, Mingxia; Liu, Yuan; Wang, Xiaoxia; Fu, Hui; Wang, Shiyao; Liu, Xueying; Shi, Lianxuan

doi:10.1111/ppl.13022

Cited by 27 publications

(15 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The saturated and monounsaturated fatty acids accumulated when the N concentration was decreased [ 45 ]. It has been reported that N-deficient-tolerant soybean maintained higher chlorophyll content, as well as increased fatty acid metabolism [ 46 ]. Recently, in cultivated wheat, there were close relationships between the alteration in membrane lipid and fatty acid compositions and the plant N-deficiency response under N deficiency condition [ 31 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings

et al. 2020

View full text Add to dashboard Cite

Background Nitrogen (N) deficiency is a major constraint for plant production in many areas. Developing the new crop genotypes with high productivity under N deficiency is an important approach to maintain agricultural production. Therefore, understanding how plant response to N deficiency and the mechanism of N-deficiency tolerance are very important for sustainable development of modern crop production. Results In this study, the physiological responses and fatty acid composition were investigated in 24 wheat cultivars under N-deficient stress. Through Pearson’s correlation analysis and principal component analysis, the responses of 24 wheat cultivars were evaluated. The results showed that the plant growth and carbohydrate metabolism were all differently affected by N deficiency in all tested wheat cultivars. The seedlings that had high shoot biomass also maintained high level of chlorophyll content under N deficiency. Moreover, the changes in fatty acid composition, especially the linolenic acid (18:3) and the double bond index (DBI), showed close positive correlations with the shoot dry weight and chlorophyll content alterations in response to N-deficient condition. These results indicated that beside the chlorophyll content, the linolenic acid content and DBI may also contribute to N-deficiency adaptation, thus could be considered as efficient indicators for evaluation of different response in wheat seedlings under N-deficient condition. Conclusions The alteration in fatty acid composition can potentially contribute to N-deficiency tolerance in plants, and the regulation of fatty acid compositions maybe an effective strategy for plants to adapt to N-deficient stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The energy was -70 eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 50-500 at a rate of 12.5 spectra per s after a solvent delay of 6.30 min (Zhao et al 2019).…”

Section: Gc-tof-ms Analysismentioning

confidence: 99%

“…Wild soybean (Glycine soja ) is the related species of cultivated soybean, which has a higher resistance to disease, insect, drought, and nutrient deficiency than cultivated soybean. Our previous results found that wild soybean can tolerate low nitrogen and drought stresses by maintaining nutrient balance and stable tricarboxylic acid (TCA) cycle and increasing the concentration of metabolites such as amino acids (Wanget al 2019;Zhao et al 2019). Nevertheless, the adaptation mechanism of wild soybean and cultivated soybean under low P conditions is still not very clear.…”

Section: Introductionmentioning

confidence: 99%

Nutrient reabsorption mechanism adapted to low phosphorus in wild and cultivated soybean varieties

Shen

Guo

Zhao

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Phosphorus (P) is an essential nutrient element for plants. Wild soybean (Glycine soja) expresses higher tolerance to P-limited environment compared to cultivated soybeans (Glycine max). In this study, the response of ionomics and metabonomics in young and old leaves of two soybean varieties under low P were studied. Our results showed that the tolerance of low P in wild soybean can be improved by promoting the accumulation of Mg2+, Fe3+, and SO42- in young and old leaves and the transportation of NO3− and H2PO4− from old to young leaves. The young and old leaves of wild soybean under low P accumulated sugars including maltose and sucrose, amino acids including asparagine and glutamine, and nitrogenous compounds including tyramine, and enhanced the tricarboxylic acid cycle metabolism, especially in young leaves, but decreased the content of hexose-phosphate metabolites. Our experiment indicated that wild soybean can tolerate low P by enhancing the energy metabolism in young and old leaves, promoting the transportation and reuse of sugars and amino acid metabolites from old to young leaves, and mobilizing Pi from hexose-phosphate of old leaves to young leaves. Our results provide a new insight for the cultivation of new soybean varieties with tolerance to P deficiency.

show abstract

“…Moreover, the TCA cycle, shikimic acid pathway, synthetase/glutamate synthase (GS/GOGAT) cycle, and accumulation of most organic acids were enhanced in the low N-tolerant genotype of wild soybean (Glycine soja Sieb.) but reduced in the common genotype (Zhao et al, 2020). In wild barley (Hordeum spontaneum L.), low N affected the glycolysis, TCA, and pentose phosphate pathways, while the tolerant genotype accumulated energysaving amino acids, distributed carbon to roots, and had a higher capacity to maintain redox homeostasis (Quan et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

et al. 2022

View full text Add to dashboard Cite

Kentucky bluegrass (Poa pratensis L.) is a cool-season turfgrass species that responds strongly to nitrogen (N), but the metabolomic responses of this grass species to N supply is unknown. The N-tolerant cultivar Bluemoon and N-sensitive cultivar Balin were exposed to normal N (15 mM) and low N (0.5 mM) for 21 days for identification of differentially expressed metabolites (DEMs) between normal N and low N treatments. Balin had more reductions of chlorophyll and total soluble protein concentrations and a higher accumulation of superoxide radicals under low N stress. A total of 99 known DEMs were identified in either cultivar or both including 22 amino acids and derivatives, 16 carbohydrates, 29 organic acids, and 32 other metabolites. In Bluemoon, β-alanine metabolism was most enriched, followed by alanine, aspartate, and glutamate metabolism, biosynthesis of valine, leucine, and isoleucine biosynthesis, and glycine, serine, and threonine metabolism. In Balin, alanine, aspartate, and glutamate metabolism were most enriched, followed by the tricarboxylic acid (TCA), glyoxylate and decarbohydrate metabolism, and carbon fixation. Bluemoon generally maintained higher TCA cycle capacity and had more downregulated amino acids, while changes in more organic acids occurred in Balin under low N stress. Some metabolite changes by low-N stress were cultivar-specific. The results suggested that regulation of metabolites related to energy production or energy saving could contribute to low N tolerance in Kentucky bluegrass.

show abstract

Physiological characteristics and metabolomics reveal the tolerance mechanism to low nitrogen in Glycine soja leaves

Cited by 27 publications

References 62 publications

Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings

Comprehensive evaluation of physiological traits under nitrogen stress and participation of linolenic acid in nitrogen-deficiency response in wheat seedlings

Nutrient reabsorption mechanism adapted to low phosphorus in wild and cultivated soybean varieties

Differential Metabolomic Responses of Kentucky Bluegrass Cultivars to Low Nitrogen Stress

Contact Info

Product

Resources

About