Physiological characterization of anaerobic ammonium oxidizing bacterium ‘<scp><i>C</i></scp><i>andidatus</i> <scp>J</scp>ettenia caeni’

Ali, Muhammad; Oshiki, Mamoru; Awata, Takanori; Isobe, Kazuo; Kimura, Zen‐ichiro; Yoshikawa, Hiroaki; Hira, Daisuke; Kindaichi, Tomonori; Satoh, Hisashi; Fujii, Tomoyuki; Okabe, Satoshi

doi:10.1111/1462-2920.12674

Cited by 215 publications

(95 citation statements)

References 124 publications

(177 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In the present study, we showed the intracellular localization of the hydroxylamine oxidoreductase (HAO)-like protein kustc0457/58, hydrazine dehydrogenase (HDH), the NO-producing HAO-like protein HOX, nitrite oxidoreductase (NXR), and hydrazine synthase (HZS) within an anammox cell. All of these proteins are encoded in all presently available anammox genomes and play key roles in anammox catabolism (1,2,(9)(10)(11)(12)(13)(14). The fact that all studied protein complexes were exclusively immunolocalized to the anammoxosome in the present study supported the hypothesis that this cell compartment was a true prokaryotic organelle with a dedicated function.…”

Section: Discussionsupporting

confidence: 81%

See 1 more Smart Citation

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

et al. 2015

View full text Add to dashboard Cite

Anaerobic ammonium-oxidizing (anammox) bacteria oxidize ammonium with nitrite as the terminal electron acceptor to form dinitrogen gas in the absence of oxygen. Anammox bacteria have a compartmentalized cell plan with a central membrane-bound "prokaryotic organelle" called the anammoxosome. The anammoxosome occupies most of the cell volume, has a curved membrane, and contains conspicuous tubule-like structures of unknown identity and function. It was suggested previously that the catalytic reactions of the anammox pathway occur in the anammoxosome, and that proton motive force was established across its membrane. Here, we used antibodies raised against five key enzymes of the anammox catabolism to determine their cellular location. The antibodies were raised against purified native hydroxylamine oxidoreductase-like protein kustc0458 with its redox partner kustc0457, hydrazine dehydrogenase (HDH; kustc0694), hydroxylamine oxidase (HOX; kustc1061), nitrite oxidoreductase (NXR; kustd1700/03/04), and hydrazine synthase (HZS; kuste2859-61) of the anammox bacterium Kuenenia stuttgartiensis. We determined that all five protein complexes were exclusively located inside the anammoxosome matrix. Four of the protein complexes did not appear to form higher-order protein organizations. However, the present data indicated for the first time that NXR is part of the tubule-like structures, which may stretch the whole length of the anammoxosome. These findings support the anammoxosome as the locus of catabolic reactions of the anammox pathway. IMPORTANCEAnammox bacteria are environmentally relevant microorganisms that contribute significantly to the release of fixed nitrogen in nature. Furthermore, the anammox process is applied for nitrogen removal from wastewater as an environment-friendly and cost-effective technology. These microorganisms feature a unique cellular organelle, the anammoxosome, which was proposed to contain the energy metabolism of the cell and tubule-like structures with hitherto unknown function. Here, we purified five native enzymes catalyzing key reactions in the anammox metabolism and raised antibodies against these in order to localize them within the cell. We showed that all enzymes were located within the anammoxosome, and nitrite oxidoreductase was located exclusively at the tubule-like structures, providing the first insights into the function of these subcellular structures.

show abstract

Section: Discussionsupporting

confidence: 81%

“…Apart from nitrite reductase, all currently available anammox genomes encode close homologs of the proteins described above (2,(9)(10)(11)(12)(13)(14), suggesting that they have the same energy metabolism as K. stuttgartiensis.…”

mentioning

confidence: 99%

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

et al. 2015

View full text Add to dashboard Cite

show abstract

“…At present, it is unclear whether the essential nitrogen transforming enzymes utilize iron or copper cofactors. Phylogenetic analysis has identified both iron and copper binding domains in the genome of annamox bacteria (Ali et al, 2015), and copper limitation of anammox activity has been observed in the field (Jacquot et al, 2013). The evolutionary history of anammox is also poorly known.…”

Section: Anaerobic Ammonium Oxidation (Anammox)mentioning

confidence: 99%

The evolution of Earth's biogeochemical nitrogen cycle

Stüeken

Kipp

Koehler

et al. 2016

Earth-Science Reviews

328

287

View full text Add to dashboard Cite

Nitrogen is an essential nutrient for all life on Earth and it acts as a major control on biological productivity in the modern ocean. Accurate reconstructions of the evolution of life over the course of the last four billion years therefore demand a better understanding of nitrogen bioavailability and speciation through time. The biogeochemical nitrogen cycle has evidently been closely tied to the redox state of the ocean and atmosphere. Multiple lines of evidence indicate that the Earth"s surface has passed in a non-linear fashion from an anoxic state in the Hadean to an oxic state in the later Phanerozoic. It is therefore likely that the nitrogen cycle has changed markedly over time, with potentially severe implications for the productivity and evolution of the biosphere. Here we compile nitrogen isotope data from the literature and review our current understanding of the evolution of the nitrogen cycle, with particular emphasis on the Precambrian. Combined with recent work on redox conditions, trace metal availability, sulfur and iron cycling on the early Earth, we then use the nitrogen isotope record as a platform to test existing and new hypotheses about biogeochemical pathways that may have controlled nitrogen availability through time. Among other things, we conclude that (a) abiotic nitrogen sources were likely insufficient to sustain a large biosphere, thus favoring an early origin of biological N 2 fixation, (b) evidence of nitrate in the Neoarchean and Paleoproterozoic confirm current views of increasing surface oxygen levels at those times, (c) abundant ferrous iron and sulfide in the midPrecambrian ocean may have affected the speciation and size of the fixed nitrogen reservoir, and (d) nitrate availability alone was not a major driver of eukaryotic evolution.

show abstract

“…from Ca 'Jettenia sp. ', the characteristics of the maximum specific growth rate (µ max ) and the half-saturation constants (Ks NH4 and Ks NO2 for NH 4 + and NO 2 ‾, respectively) are compared [60]. parameters [1].…”

Section: Detection Of Anammox Bacteriamentioning

confidence: 99%

Enrichment of ANAMMOX bacteria from conventional activated sludge entrapped in poly(vinyl alcohol)/sodium alginate gel

Bae

Choi

Lee

et al. 2015

Chemical Engineering Journal

View full text Add to dashboard Cite

Physiological characterization of anaerobic ammonium oxidizing bacterium ‘Candidatus Jettenia caeni’

Cited by 215 publications

References 124 publications

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

The evolution of Earth's biogeochemical nitrogen cycle

Enrichment of ANAMMOX bacteria from conventional activated sludge entrapped in poly(vinyl alcohol)/sodium alginate gel

Contact Info

Product

Resources

About