Physiological characterization of <i>Colletotrichum gloeosporioides</i> causing anthracnose of pomegranate

Dev, Devanshu; Somasekhara, Y. M.

doi:10.5958/2230-7338.2018.00035.6

“…The effect of different temperature, pH value and illumination on the growth rate of pathogenic fungi, as well as the lethal temperature and suitable carbon and nitrogen sources were examined (Dev and Somasekhara, 2018;Liu et al, 2021). In the assays of the effect of different temperature, pH, and light on the growth of mycelium, same isolates, volume of medium, inoculation method, and standard of measurement were uniformed.…”

Section: Temperature Ph and Lightmentioning

confidence: 99%

Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) post-harvest anthracnose from Vietnam

Tao,

Yang,

Lin

et al. 2024

Mol Biol Rep

0

1

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Aims: To elucidate the taxonomic status and biological characteristics of the pathogens causing anthracnose of postharvest mango fruit (cultivar Okrong) from Vietnam. Methods and ResultsBased on the morphological features, along with the phylogenetic tree of ITS and ApMat combined sequences, two strains DM-1 and DM-2 isolated from the fruit of Okrong from Vietnam were identi ed as Colletotrichum fructicola and C. asianum respectively, which were the pathogens of postharvest fruit anthracnose of mango from Vietnam. The growth status of different Colletotrichum strains under different conditions were analyzed to reveal the biological characteristics of different pathogens. The optimum growth temperature of DM-1 and DM-2 was 28 °C with mycelia grow fastest in the dark. Both strains could grow when pH was 4-11, and the optimum pH value was 6. Maltose and soluble starch were the most suitable carbon source for DM-1 and DM-2 respectively, and the peptone was the most suitable nitrogen source for both strains. The lethal temperatures were recorded as 55 °C 5 min for DM-1, and 50 °C 10 min for DM-2.Conclusions: To the best of our knowledge, It is the rst time to con rmed that the pathogens of postharvest fruit anthracnose of mango from Vietnam were C. fructicola and C. asianum with different biological characteristics. Signi cance and Impact of the StudyThis study delivers the rst assessment of C. fructicola and C. asianum related to mango postharvest anthracnose in Vietnam, which contributes to the developing prevention and control measures of mango postharvest anthracnose to producer and trader of Vietnam and China.Additionally, the phytochemicals from particular anatomical part of the mango plant, such as leaf, bark, heartwood, fruit peel, pulp and seed, have potential therapeutic effects on cancer (Mirza et al., 2021).Mango is worthy of "the king of fruits".China was the second largest mango producer around the world in 2018 next to India(Konsue et al., 2020). Nowadays, mango is widely cultured in southern China with various cultivars. Among of them, an early-maturing variety, Okrong , originated from Vietnam, is generally consumed due to its juiciness, delicious taste, small pit and less ber(He et al., 2020), which has been cultivated in Yunnan, Guangxi, Sichuan, Hainan and other mango growing areas of China in recent years. China is also the biggest

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Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) postharvest anthracnose from Vietnam

Tao,

Yang,

Lin

et al. 2024

Preprint

0

View full text Add to dashboard Cite

Aims: To elucidate the taxonomic status and biological characteristics of the pathogens causing anthracnose of postharvest mango fruit (cultivar Okrong) from Vietnam. Methods and Results：Based on the morphological features, along with the phylogenetic tree of ITS and ApMat combined sequences, two strains DM-1 and DM-2 isolated from the fruit of Okrong from Vietnam were identified as Colletotrichum fructicola and C. asianum respectively, which were the pathogens of postharvest fruit anthracnose of mango from Vietnam. The growth status of different Colletotrichum strains under different conditions were analyzed to reveal the biological characteristics of different pathogens. The optimum growth temperature of DM-1 and DM-2 was 28 °C with mycelia grow fastest in the dark. Both strains could grow when pH was 4-11, and the optimum pH value was 6. Maltose and soluble starch were the most suitable carbon source for DM-1 and DM-2 respectively, and the peptone was the most suitable nitrogen source for both strains. The lethal temperatures were recorded as 55 °C 5 min for DM-1, and 50 °C 10 min for DM-2. Conclusions: To the best of our knowledge, It is the first time to confirmed that the pathogens of postharvest fruit anthracnose of mango from Vietnam were C. fructicola and C. asianum with different biological characteristics.

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Predictive Model for the Effect of Environmental Conditions on the Postharvest Development of Colletotrichum gloeosporioides Strains Isolated from Papaya (Carica papaya L.)

Sandoval-Contreras

¹

,

Iñiguez‐Moreno

²

,

Garrido-Sánchez

³

et al. 2020

Journal of Food Protection

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Colletotrichum species are the most important postharvest spoilage of papaya fruit. The objective of this research was to evaluate the effect of temperature and relative humidity on growth rate and time for growth to become visible of five strains of Colletotrichum gloeosporioides isolated from papaya fruit in a complex medium. As a primary model, the radial growth rates were estimated using the Baranyi and Roberts model in papaya agar. The Solver Excel function was used to obtain the time for visible mycelium (tv). Secondary models obtained with the Rosso’s cardinal model with inflection were applied to describe the effect of temperature on radial growth rate (µ, mm/d). The Arrhenius-Davey model was used to model tv. The obtained models seem to be satisfactory for describing the µ and time tv. The relative humidity (RH) had influence on µ and time tv for all tested C. gloeosporioides isolates, but no model is able to describe the behavior of the fungus. External validation of models on papaya fruit was performed. Growth models were developed with the same models used in vitro. The bias (Bf) and the accuracy factors (Af), as indices for performance evaluation of predictive models in food microbiology, as a function of temperature and RH, were 1.22 and 1.33 for growth rate, and 1.18 and 1.62 for time for visible mycelium respectively indicating good predictions. The supply chain of papaya is complex and require constant conditions and mismatches in can cause damage to fruit. Knowledge of the behavior of C. gloeosporioides on papaya fruit and application of the developed models at the supply chain level will help to establish transport control strategies against these molds. This research has contributed to the first models of growth for C. gloeosporioides in Mexico.

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Physiological characterization of Colletotrichum gloeosporioides causing anthracnose of pomegranate

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Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) post-harvest anthracnose from Vietnam

Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) post-harvest anthracnose from Vietnam

Biological characterization of emerging fungal pathogen Colletotrichum associated with mango (Mangifera indica L.) postharvest anthracnose from Vietnam

Predictive Model for the Effect of Environmental Conditions on the Postharvest Development of Colletotrichum gloeosporioides Strains Isolated from Papaya (Carica papaya L.)

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