Physiological effects of copper in the euryhaline copepod Acartia tonsa: Waterborne versus waterborne plus dietborne exposure

Pinho, Grasiela Lopes Leães; Pedroso, Mariana Saia; Rodrigues, Sandra Carvalho; Souza, Sandra Silvestre de; Bianchini, Adalto

doi:10.1016/j.aquatox.2007.06.001

Cited by 47 publications

(45 citation statements)

References 21 publications

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“…This fact may be related to their omnivorous feeding behavior, being able to survive and reproduce under different diets (Kleppel 1992, Saiz et al Correspondence to: Tatiana Ramos Avila E-mail: tatioceano@yahoo.com.br 2007). Such characteristic makes Acartia species relatively easy to cultivate at small-and large-scale under laboratory conditions, providing enough biomass for use as an alternative live food in marine aquaculture (Støttrup andNosker 1997, McKinnon et al 2003) and a biological model in toxicological tests (Bielmyer et al 2006, Pinho et al 2007.…”

Section: Introductionmentioning

confidence: 99%

Diet influence on egg production of the copepod Acartia tonsa (Dana, 1896)

Teixeira

Kaminski

Avila

et al. 2010

An. Acad. Bras. Ciênc.

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Egg production in the copepod Acartia tonsa was evaluated using different densities of the microalgae Thalassiosira weissflogii, Chaetoceros muelleri and Isochrysis galbana. Male and female were kept under controlled conditions (salinity 30, 20 • C, photoperiod 12L:12D), acclimated to the experimental conditions and left over a period of 24 h to allow copulation. Algal densities tested were equivalent in biovolume and corresponded to 0, 2.5, 5, 10, 20, 40 and 60.10 3 cells.mL −1 of T. weissflogii. Ten acclimated female were separated, transferred to glass bottles and exposed for further 24 h to the corresponding experimental medium. After this period, the eggs were fixed and counted. Copepod egg production reached a threshold value when T. weissflogii, C. muelleri and I. galbana were supplied at 10.10 3 , 140.10 3 and 640.10 3 cells.mL −1 , respectively. Mean egg production corresponded to 28.0 ± 0.5, 20.1 ± 1.0 and 22.0 ± 3.5 eggs.female −1 .day −1 , respectively. Copepods fed T. weissflogii showed the highest mean egg production while those fed I. galbana reached a maximum egg production when the algae were supplied at a density two-to fourfold higher, considering the biovolume of T. weissflogii and C. muelleri. These differences are explained considering the different sizes of the microalgae used to feed the copepods.

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Section: Introductionmentioning

confidence: 99%

Diet influence on egg production of the copepod Acartia tonsa (Dana, 1896)

Teixeira

Kaminski

Avila

et al. 2010

An. Acad. Bras. Ciênc.

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“…No significant difference in Cu accumulation was observed in the copepod Acartia tonsa exposed to Cu in both water and diet at salinities of 15 and 30, and in water-only exposures, Cu accumulation was only 23% higher at a salinity of 15 compared with 30 (Pinho et al 2007). Clearly salinity cannot explain the more than twofold range in Cu concentrations in plume copepods collected over the range of salinities in this study (23.5-31.5 in April 2005 and26.1-32.2 in May 2006).…”

Section: Discussionmentioning

confidence: 88%

The influence of river plume dynamics on trace metal accumulation in calanoid copepods

Wright

Frazer

Reinfelder

2010

Limnology & Oceanography

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The concentrations of Ag, Cd, Cu, Hg(II), monomethylmercury (MeHg), Pb, and Zn were measured and modeled in calanoid copepods collected from the Hudson River plume in April 2005 and May 2006. Copepods in the buoyant plume were significantly (p , 0.05) enriched in Ag, Cu, Pb, Zn, and MeHg (2-to 25-fold), but depleted in Cd (by 25-40%) relative to oceanic copepods. Median burdens of Ag, Cu, and Hg(II) differed in plume copepods collected in April 2005 and May 2006 by factors of 1.5 to 3, reflecting the influence of river discharge and associated physical processes on plankton dynamics and metal bioaccumulation. Only the concentrations of Cu and Zn in plume copepods clearly showed the expected trend of highest levels near the mouth of the Hudson River and lower levels offshore. Spatial trends in concentrations of Ag and Hg(II) in plume copepods were strikingly different from those in suspended particles and the dissolved phase, apparently reflecting differences in metal bioavailability because of shifts in available food types. Modeled concentrations of Cd, Cu, Pb, and Zn in plume copepods were within less than a factor of two of measured values, but model results showed that Hg(II) was less bioavailable, and Ag and MeHg were more bioavailable in the Hudson River plume than laboratory results would predict. Zooplankton from urban river plumes may accumulate potentially toxic levels of trace metals or act as a trophic link between metal contamination in polluted estuaries and pelagic ecosystems.

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“…For M. mactroides samples, Cu and Na ? concentration was analyzed by atomic absorption spectrophotometry (AAS 932 Avanta Plus, GBC, Hampshire, IL, USA), as previously described (Pinho et al 2007;Lopes et al 2011b). Cell Cu or Na ?…”

Section: Cell Distinctionmentioning

confidence: 99%

“…uptake (Goss et al 2001;Galvez et al 2002;Reid et al 2003), and particularly in analyzing the ionoregulatory disrupting effects of metals such as Cu (Goss et al 2011). Cu is now generally recognized to be an ionoregulatory toxicant in aquatic invertebrates (Pinho et al 2007;Lopes et al 2011a, b) as well as in fish. Therefore, we exposed gill cells of Fraction II isolated from the freshwater mussel L. costata and the seawater clam M. mactroides to three environmentally relevant concentrations of copper (Cu) (5, 9 and 20 lg Cu/L) for 3 h and analysed the cellular content of both Cu and Na ?…”

Section: Introductionmentioning

confidence: 99%

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

Nogueira¹,

Wood

Gillis

et al. 2013

Cytotechnology

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Gills cells of the freshwater mussel Lasmigona costata and the seawater clam Mesodesma mactroides were isolated (mussel: chemical dissociation; clam: mechanical dissociation) and fractionated (Percoll gradient) into Fractions I and II. Mitochondrial dyes (DASPEI: mussel; MitoTracker Ò : clam) and Na ? , K ? -ATPase activity measurement were used to distinguish between cells of Fractions I and II. For mussel and clam, 80.5 ± 1.5 and 48.3 ± 3.2 % of cells were in Fraction II, respectively. For both species, cells of Fraction II had higher fluorescence emission and higher enzyme activity than those of Fraction I, being characterized as 'cells rich in mitochondria'. Cells of Fraction II were kept in saline solutions approximating the ionic composition of hemolymph either under control conditions (no Cu addition) or exposed (3 h) to copper (Cu: 5, 9 and 20 lg Cu/L). Cell viability and Cu and Na ? content were measured. For both species, Cu content was higher and Na ? content was lower in cells exposed to 20 lg Cu/L. Furthermore, a strong negative correlation was observed between cell Na ? and Cu content in the two bivalve species, indicating a possible competition between Cu and Na ? for ion-transporting mechanisms or binding sites at gill cells of Fraction II. Considering that Cu is an ionoregulatory toxicant in aquatic invertebrates, these preliminary toxicological data support the idea of using isolated gill cells rich in mitochondria to study the mechanisms underlying the acute toxicity of waterborne Cu in freshwater and marine bivalves.

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Physiological effects of copper in the euryhaline copepod Acartia tonsa: Waterborne versus waterborne plus dietborne exposure

Cited by 47 publications

References 21 publications

Diet influence on egg production of the copepod Acartia tonsa (Dana, 1896)

Diet influence on egg production of the copepod Acartia tonsa (Dana, 1896)

The influence of river plume dynamics on trace metal accumulation in calanoid copepods

Isolation and fractionation of gill cells from freshwater (Lasmigona costata) and seawater (Mesodesma mactroides) bivalves for use in toxicological studies with copper

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