Physiological functions of Atg6/Beclin 1: a unique autophagy-related protein

Cao, Yang; Klionsky, Daniel J.

doi:10.1038/cr.2007.78

Cited by 513 publications

(414 citation statements)

References 86 publications

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“…Effect of combined ectopic Beclin-1 expression and rapamycin treatment on autophagy induction and cell growth Increased Beclin-1 expression regulates autophagy tightly by initiating autophagosome formation (Cao and Klionsky, 2007). Ectopic expression of Beclin-1 failed to modulate apoptosis (Fig.…”

Section: Resultsmentioning

confidence: 95%

Targeting the Autophagy Pathway Using Ectopic Expression of Beclin 1 in Combination with Rapamycin in Drug-Resistant v-Ha-ras-Transformed NIH 3T3 Cells

Eum

Lee

2011

Molecules and Cells

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Section: Resultsmentioning

confidence: 95%

Targeting the Autophagy Pathway Using Ectopic Expression of Beclin 1 in Combination with Rapamycin in Drug-Resistant v-Ha-ras-Transformed NIH 3T3 Cells

Eum

Lee

2011

Molecules and Cells

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“…Beclin-1 is the first identified autophagy-related gene that could reflect the level of autophagy (Cao and Klionsky, 2007;Zeng et al, 2006). It plays a crucial role in the process of tumorigenesis.…”

Section: Discussionmentioning

confidence: 99%

“…Beclin-1, the mammalian orthologue of the yeast Apg6/ Vps30 gene, is considered as the first identified autophagy gene product (Zeng et al, 2006;Cao and Klionsky, 2007). It is involved in nucleation and locus on chromosome 17q21, which is isolated as a Bcl-2-interacting protein (Miracco et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Prognostic Significance of Beclin-1 Expression in Colorectal Cancer: a Meta-analysis

Han

Xue²,

Shen³

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Objective: Beclin-1 has recently been observed as an essential marker of autophagy in several cancers. However, the prognostic role of Beclin-1 in colorectal neoplasia remains controversial. Our study aimed to evaluate the potential association between Beclin-1 expression and the outcome of colorectal cancer patients. Materials and Methods: All related studies were systematically searched in Pubmed, Embase, Springer and Chinese National Knowledge Infrastructure databases (CNKI), and then a meta-analysis was performed to determine the association of Beclin-1 expression with clinical outcomes. Finally, a total of 6 articles were included in our analysis. Results: Our data showed that high Beclin-1 expression in patients with CRC was associated with poor prognosis in terms of tumor distant metastasis (OR=2.090, 95%CI=1.061-4.119, p=0.033) and overall survival (RR=1.422, 95%CI=1.032-1.959, p=0.031). However, we did not found any correlation between Beclin-1 over-expression and tumor differentiation (OR=1.711, 95%CI=0.920-3.183, p=0.090). In addition, there was no evidence of publication bias as suggested by Egger's tests for tumor distant metastasis (p=1.000), differentiation (p=1.000) and OS (p=0.308). Conclusions: Our present meta-analysis indicated that elevated Beclin-1 expression iss associated with tumor metastasis and a poor prognosis in patients with CRC. Beclin-1 might serve as an efficient prognostic indicator in CRC, and could be a new molecular target in CRC therapy.

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“…Despite autophagy being a primarily cytoprotective mechanism, excessive self-digestion can also be detrimental (Cao and Klionsky 2007;Pattingre et al 2008). The most significant genes to have been studied to date are BECLIN1 and LC3B (Chen and Karantza-Wadsworth 2009;Miracco et al 2010), with many studies having demonstrated the influence of deregulation in their expression during tumorigenesis (Levine 2007;Roy and Debnath 2010).…”

Section: Introductionmentioning

confidence: 99%

Cellular senescence and autophagy of myoepithelial cells are involved in the progression of in situ areas of carcinoma ex-pleomorphic adenoma to invasive carcinoma. An in vitro model

Silva

Martinez

Demasi

et al. 2015

J. Cell Commun. Signal.

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During tumor invasion, benign myoepithelial cells of carcinoma ex-pleomorphic adenoma (CXPA) surround malignant epithelial cells and disappear. The mechanisms involved in the death and disappearance of these myoepithelial cells were investigated via analysis of the expression of regulatory proteins for apoptosis, autophagy and cellular senescence in an in situ in vitro model. Protein expression relating to apoptosis (Bax, Bcl-2, Survivin), autophagy (Beclin-1, LC3B) and cellular senescence (p21, p16) was evaluated using indirect immunofluorescence. β-galactosidase expression was assessed via histochemistry. Biopsies of CXPA (ex vivo) allowed immunhistochemical evaluation of p21 and p16, whilst LC3B, p21 and p16 protein expression was analyzed by western blotting. In the in vitro model, the myoepithelial cells were positive for LC3B (cytoplasm) and p21 (nucleus), whilst in vivo positivity for p21 and p16 was observed. In vitro, β-galactosidase activity increased in the myoepithelial cells over time. Western blotting analysis revealed an increased LC3B, p16 and p21 expression in the myoepithelial cells with previous contact with the malignant cells when compared with those without contact. The investigation of behavior of benign myoepithelial cells in ductal areas of CXAP revealed that the myoepithelial cells are involved in the autophagy-senescence phenotype that subsequently leads to their disappearance.

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Physiological functions of Atg6/Beclin 1: a unique autophagy-related protein

Cited by 513 publications

References 86 publications

Targeting the Autophagy Pathway Using Ectopic Expression of Beclin 1 in Combination with Rapamycin in Drug-Resistant v-Ha-ras-Transformed NIH 3T3 Cells

Targeting the Autophagy Pathway Using Ectopic Expression of Beclin 1 in Combination with Rapamycin in Drug-Resistant v-Ha-ras-Transformed NIH 3T3 Cells

Prognostic Significance of Beclin-1 Expression in Colorectal Cancer: a Meta-analysis

Cellular senescence and autophagy of myoepithelial cells are involved in the progression of in situ areas of carcinoma ex-pleomorphic adenoma to invasive carcinoma. An in vitro model

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