Physiological response of grapevine cultivars and a rootstock to infection with Phaeoacremonium and Phaeomoniella isolates: an in vitro approach using plants and calluses

Santos, Conceição; Fragoeiro, Sílvia; Phillips, Alan J. L.

doi:10.1016/j.scienta.2004.04.023

Cited by 42 publications

(34 citation statements)

References 12 publications

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“…In the case of esca, such a shift in fungal community structure is not observed. In our study, however, a single fungal OTU (based on ITS similarity) possibly embraces very closely related species, subspecies or strains that have a different virulence and could be differentially associated with healthy or diseased plants, as for instance in the case of Alternaria (Table 1, Pryor and Michailides 2002), Phaeomoniella chlamydospora (Mostert et al 2006) or Phaeoacremonium angustius (Santos et al 2005). Also, cumulated small differences in abundance of several OTUs might eventually differentiate between healthy and diseased plants, but such slight differences in abundance are, each taken separately, too small to contribute to a significant distinction between healthy and diseased plants in a PCA analysis (Fig.…”

Section: Discussionmentioning

confidence: 89%

“…Other organisms, such as bacteria, may be involved in esca but eventual differences between the bacterial communities associated with diseased or healthy grapevines have never been studied. As suggested by Bertsch et al (2009), environmental parameters may also play an important role in the emergence of grapevine trunk diseases, as may changes in vineyard management and cultural practices (Graniti et al 2000) or differences between grapevine genotypes (Santos et al 2005).…”

Section: Discussionmentioning

confidence: 99%

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What if esca disease of grapevine were not a fungal disease?

et al. 2012

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Esca disease, which attacks the wood of grapevine, has become increasingly devastating during the past three decades and represents today a major concern in all wine-producing countries. This disease is attributed to a group of systematically diverse fungi that are considered to be latent pathogens, however, this has not been conclusively established. This study presents the first in-depth comparison between the mycota of healthy and diseased plants taken from the same vineyard to determine which fungi become invasive when foliar symptoms of esca appear. An unprecedented high fungal diversity, 158 species, is here reported exclusively from grapevine wood in a single Swiss vineyard plot. An identical mycota inhabits wood of healthy and diseased adult plants and presumed esca pathogens were widespread and occurred in similar frequencies in both plant types. Pioneer esca-associated fungi are not transmitted from adult to nursery plants through the grafting process. Consequently the presumed esca-associated fungal pathogens are most likely saprobes decaying already senescent or dead wood resulting from intensive pruning, frost or other mecanical injuries as grafting. The cause of esca disease therefore remains elusive and requires well executive scientific study. These results question the assumed pathogenicity of fungi in other diseases of plants or animals where identical mycota are retrieved from both diseased and healthy individuals.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

What if esca disease of grapevine were not a fungal disease?

et al. 2012

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“…Scarpari et al (2005) reported reductions in chlorophyll a and b in Theobroma cacao plants infected by the pathogen Crinipellis perniciosa. Santos et al (2005) also described the consequences of Phaeoacremonium angustius and Phaeomoniella chlamydospora infection in two Vitis vinifera cultivars.…”

Section: Photosynthesis and Photosynthetic Water Use Efficiencymentioning

confidence: 99%

Relationships between leaf pigments and photosynthesis in common bean plants infected by anthracnose

Lobato

Gonçalves‐Vidigal

Filho

et al. 2010

New Zealand Journal of Crop and Horticultural Science

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The objective of this study was to: first, evaluate the infection effects caused by Colletotrichum lindemuthianum pathogen (race 2047) on photosynthetic pigments and gas exchanges in Phaseolus vulgaris plants (cv. Mexico 222); and, second, determine infection effects on leaf pigments and their consequences on photosynthesis rate. A completely randomized design with a factorial scheme was used, combining two treatments (control and inoculated) and three evaluation periods (4th, 8th and 12th day). Carotenoid levels presented decreases of 28.3% and 35% during the 8th and 12th day after infection, when control and inoculated plants were compared. Correlation analysis demonstrated the direct relationship between carotenoids and photosynthesis rate (r 00.84). Total chlorophyll in infected plants had progressive reductions of 6.4%, 20.6% and 21.3% on the 4th, 8th and 12th day, respectively, when treated and untreated plants were compared. Total chlorophyll with photosynthesis (r 00.85) also revealed a significant and linear correlation. The photosynthetic rate in infected plants decreased by 22%, 49.9% and 77.3% on the 4th, 8th and 12th days after the inoculation, respectively. Anthracnose infection also induced negative effects concerning stomatal conductance, transpiration, photosynthesis and water use efficiency. Our results demonstrate that leaf pigment reduction as a result of pathogens was the main cause of lower gaseous exchanges in infected plants.

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“…Calli or cell suspensions are often used to study the expression of defence-related genes in different diseases or to study the effect of elicitors. Even some studies concerning esca disease have used in vitro cultured cells (calli) to understand the inhibitory effect of toxins from P. aleophilum (teleomorph form: Togninia minima) or P. chlamydospora (teleomorph unknown) (Sparapano et al 2001;Santos et al 2005). Hence, it seems that this model allows us to study the different defence pathways from Vitis cells under controlled conditions.…”

Section: Introductionmentioning

confidence: 99%

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

et al. 2014

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Three major grapevine trunk diseases, esca, botryosphaeria dieback and eutypa dieback, pose important economic problems for vineyards worldwide, and currently, no efficient treatment is available to control these diseases. The different fungi associated with grapevine trunk diseases can be isolated in the necrotic wood, but not in the symptomatic leaves. Other factors seem to be responsible for the foliar symptoms and may represent the link between wood and foliar symptoms. One hypothesis is that the extracellular compounds produced by the fungi associated with grapevine trunk diseases are responsible for pathogenicity.In the present work, we used Vitis vinifera cv. Chardonnay cells to test the aggressiveness of total extracellular compounds produced by Diplodia seriata and Neofusicoccum parvum, two causal agents associated with botryosphaeria dieback. Additionally, the toxicity of purified mellein, a characteristic toxin present in the extracellular compounds of Botryosphaeriaceae, was assessed.Our results show that the total extracellular compounds produced by N. parvum induce more necrosis on Chardonnay calli and induce a different defence gene expression pattern than those of D. seriata. Mellein was produced by both fungi in amounts proportional to its aggressiveness. However, when purified mellein was added to the culture medium of calli, only a delayed necrosis and a lower-level expression of defence genes were observed. Extracellular compounds seem to be involved in the pathogenicity of the fungi associated with botryosphaeria dieback. However, the doses of mellein used in this study are 100 times higher than those found in the liquid fungal cultures: therefore, the possible function of this toxin is discussed.

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Physiological response of grapevine cultivars and a rootstock to infection with Phaeoacremonium and Phaeomoniella isolates: an in vitro approach using plants and calluses

Cited by 42 publications

References 12 publications

What if esca disease of grapevine were not a fungal disease?

What if esca disease of grapevine were not a fungal disease?

Relationships between leaf pigments and photosynthesis in common bean plants infected by anthracnose

Extracellular compounds produced by fungi associated with Botryosphaeria dieback induce differential defence gene expression patterns and necrosis in Vitis vinifera cv. Chardonnay cells

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