Physiological strains remodel extracellular matrix and cell–cell adhesion in osteoblastic cells cultured on alumina-coated titanium alloy

Palma, Fabrice Di; Chamson, André; Lafage-Proust, Marie-Hélène; Jouffray, Paul; Sabido, Odile; Peyroche, Sylvie; Vico, Laurence; Rattner, Aline

doi:10.1016/j.biomaterials.2003.09.026

Cited by 30 publications

(23 citation statements)

References 65 publications

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“…In contrast to these parameters, no significant differences in protein synthesis were measured in the three gel groups, suggesting that mechanical forces in general are capable of affecting cellular activity. The increased MC3T3 proliferative rate measured upon active distraction in this study has been observed previously in osteoblasts subjected to stress in 2D systems 14,16,18,[31][32][33] and in 3D model systems under isometric tension. 15,16 However, this effect in 3D systems appears to depend on the type of stress, as an inhibition in proliferation can be measured upon application of broad frequency vibration.…”

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confidence: 59%

In VitroMicrodistraction of Preosteoblasts: Distraction Promotes Proliferation and Oscillation Promotes Differentiation

et al. 2006

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Osteoblast biology is influenced in vivo by a 3-dimensional (3D) extracellular matrix that mediates their adhesion and interaction and by a constant state of compressive and tensile forces. To study the role of mechanical stress on osteoblasts in vitro, these parameters must be addressed. Therefore, this study describes the use of a novel, in vitro system that subjects cells to distractive and compressive forces in a 3D environment. This system, termed a microdistractor system, was used to apply linear forces to 3D collagen type I gels containing preosteoblasts. Gels were induced for up to 16 days in osteogenic medium and subjected to either constant linear distraction (distraction gels) or to repeating cycles of distraction and compression (oscillation gels). The effect of these stresses was evaluated over time by measuring proliferation rates, protein synthesis (i.e., cellular activity), and osteogenic differentiation levels. While linear forces in general appeared to increase protein synthesis, force-specific effects on proliferation and differentiation were observed. Specifically, distraction forces appeared to enhance MC3T3 proliferation while distraction/compressive forces appeared to accelerate their osteogenic differentiation program. Therefore, these results suggest that the microdistraction system may be an appropriate in vitro system for the study of mechanobiology in osteoblast phenotype.

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confidence: 59%

In VitroMicrodistraction of Preosteoblasts: Distraction Promotes Proliferation and Oscillation Promotes Differentiation

et al. 2006

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“…Bone cells have been shown to respond to mechanical stimuli, both fluid flow-induced shear stress and substrate strain [29]. For example osteoblasts, seeded on an alumina-coated titanium alloy, have been demonstrated to respond to physiological strains of 600 l strain by reinforcement of cell-cell adhesion and remodelling of the fibronectin network [7]. But an application of more than 10,000 l strain results in a dedifferentiation of osteoblasts and a change in cell morphology to become fibroblast-like [18].…”

Section: Discussionmentioning

confidence: 99%

Micro-CT-based screening of biomechanical and structural properties of bone tissue engineering scaffolds

et al. 2006

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The development of successful scaffolds for bone tissue engineering requires a concurrent engineering approach that combines different research fields. In order to limit in vivo experiments and reduce trial and error research, a scaffold screening technique has been developed. In this protocol seven structural and three biomechanical properties of potential scaffold materials are quantified and compared to the desired values. The property assessment is done on computer models of the scaffolds, and these models are based on micro-CT images. As a proof of principle, three porous scaffolds were evaluated with this protocol: stainless steel, hydroxyapatite, and titanium. These examples demonstrate that the modelling technique is able to quantify important scaffold properties. Thus, a powerful technique for automated screening of bone tissue engineering scaffolds has been developed that in a later stage may be used to tailor the scaffold properties to specific requirements.

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“…Previous studies indicated that mechanical strain increased matrix mineralization of osteoblasts derived from mesenchymal stem cells [8,9] and enhanced expression levels of bone ECM-related proteins/genes [10,24]. …”

Section: Discussionmentioning

confidence: 99%

Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential

et al. 2012

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BackgroundThe extracellular matrix (ECM) provides a supportive microenvironment for cells, which is suitable as a tissue engineering scaffold. Mechanical stimulus plays a significant role in the fate of osteoblast, suggesting that it regulates ECM formation. Therefore, we investigated the influence of mechanical stimulus on ECM formation and bioactivity.MethodsMouse osteoblastic MC3T3-E1 cells were cultured in cell culture dishes and stimulated with mechanical tensile strain. After removing the cells, the ECMs coated on dishes were prepared. The ECM protein and calcium were assayed and MC3T3-E1 cells were re-seeded on the ECM-coated dishes to assess osteoinductive potential of the ECM.ResultsThe cyclic tensile strain increased collagen, bone morphogenetic protein 2 (BMP-2), BMP-4, and calcium levels in the ECM. Compared with the ECM produced by unstrained osteoblasts, those of mechanically stimulated osteoblasts promoted alkaline phosphatase activity, elevated BMP-2 and osteopontin levels and mRNA levels of runt-related transcriptional factor 2 (Runx2) and osteocalcin (OCN), and increased secreted calcium of the re-seeded MC3T3-E1 cells.ConclusionMechanical strain promoted ECM production of osteoblasts in vitro, increased BMP-2/4 levels, and improved osteoinductive potential of the ECM. This study provided a novel method to enhance bioactivity of bone ECM in vitro via mechanical strain to osteoblasts.

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Physiological strains remodel extracellular matrix and cell–cell adhesion in osteoblastic cells cultured on alumina-coated titanium alloy

Cited by 30 publications

References 65 publications

In VitroMicrodistraction of Preosteoblasts: Distraction Promotes Proliferation and Oscillation Promotes Differentiation

In VitroMicrodistraction of Preosteoblasts: Distraction Promotes Proliferation and Oscillation Promotes Differentiation

Micro-CT-based screening of biomechanical and structural properties of bone tissue engineering scaffolds

Mechanical strain promotes osteoblast ECM formation and improves its osteoinductive potential

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