Physiological studies on the effect of a bradykinin potentiating factor (BPF) isolated from scorpion venom on the burnt skin of alloxan-induced diabetic Guinea pigs.

Abstract

It is well known that, Wound healing in the diabetes is enormous, growing problem and having cost Medicaid. Therefore, this study produces an exciting prospect, to improve diabetic burn healing, and evaluates the ability of a bradykinin potentiating factor (BPF) isolated from scorpion venom (Buthus occitanus) in treatment of burns in diabetic male Guinea pigs. Male Guinea pigs of approximate (550 g body weight each) were divided into five groups. In the normal group; Guinea pigs were interaperitoneally (i.p) injected with 100 µL saline solution. The second, served as control group which were injected with 100 µL saline solution then standard burns were obtained on the dorsal skin. The 3 rd , 4 th and 5 th groups were i.p. injected with (750 mg /kg, body weight) with a single dose of Alloxan, then standard burns were obtained, after that the 4 th group was treated by BPF in 100 µL saline solutions (1μg/gm. b. w.) topically and the 5 th group treated with BPF (1μg/gm. b. w.) topically and interperitonialy (1μg/gm. b. w. twice a week). Every group contains 20 animals and sacrificed at 15 and 30 days post-treatment by BPF (10 animals per each). The results are recorded after monitoring the CBC including (RBCs, WBCs, platelets, Hb content and HCT percentage), lipid profile including (total cholesterol (TC), triglyceride (TGs), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL)) and the skin antioxidant status (catalase activity (CAT) and superoxide dismutase (SOD) and serum nitric oxide (NO) beside malondialdehyde (MDA) concentration were also monitored during the study compared to normal animals. Also serum glucose level was monitored. The recorded results declared that, the treatment with BPF has shown an ameliorative effect on burn healing in diabetic animals. These observations and investigations were the pacemaker for the hypothesized ameliorating activity of BPF in the present study.

Ameliorative Effects of Propolis Extract on Some Biochemical and Hematological Parameters of Burnt Skin of Male Guinea pigs.

“…In recent years, NO has emerged as a critical molecule in wound healing, where it increases collagen content in experimental wounds (Thornton et al, 1998 andShi et al, 2000). It is worth to mention that, in other study the wound healing in diabetic guinea pigs are improved by injection of bradykinin potentiating factor (BPF) which is the stimulator of NO (Elshater et al, 2011). So in this study, the burned skin healing in guinea pigs are improved by propolis.…”

Scorpion Venom: Detriments and Benefits

“…It is estimated that a considerable number of cancer patients receive radiation therapy during their course of illness [154]. However, radiation therapy might lead to side effects, including radiation-induced heart disease (RIHD) in patients having lymphoma, breast, lung, and esophageal cancer [155,156]. It has been demonstrated that BPPs obtained from L. quinquestriatus improved cardiomyopathy induced by γ-radiation in rats, probably by acting as a scavenger of free radicals to protect the heart from negative effects derived from radiation exposure [157].…”