No abstract

The present study was investigated to evaluate the hepato-ameliorating and antioxidant activity of two aqueous extracts of Solanum nigrum (SN) against CCl 4induced toxicity in rats. Male Albino rats were divided in four groups with 8 animals in each group. Group (1) was normal group and group (2) was injected intraperitoneal (i.p.) with CCl 4 (1ml/kg) 3 times weekly for 2 weeks (control). Group (3) was injected with CCl 4 and then treated with extract from whole plant of Solanum nigrum (500 mg/kg) and group (4) was injected with CCl 4 and then treated with extract from fruits of Solanum nigrum (250 mg/kg). CCl 4 injection enhanced activity of hepatic enzymes (AST and ALT) while it decreased serum total protein and albumin in experimental animals. It also decreased RBC, platelets count, PCV and Hb levels. However it increased WBC count. CCl 4 injection increased level of lipid peroxidation resulting in a decrease in the level of enzymatic and non enzymatic antioxidants. Treatment with two extracts of Solanum nigrum altered these changes to near normal levels. But hepato-ameliorating and antioxidant effects of extract of Solanum nigrum fruits were found to be better than those of extract from whole plant of Solanum nigrum.

and is devoted to publication of original papers that elucidate important biological, chemical, or physical mechanisms logical significance. of broad physio www.eajbs.eg.net Provided for non-commercial research and education use. Not for reproduction, distribution or commercial use.

Background: Lead is a toxic heavy metal extensive in the environment. Lead induces oxidative stress. mesenchymal stem cells (MSCs) have multiplied great attention as an efficient tool in regenerative therapy. Flaxseed oil (FSO) natural source of antioxidant agents which reduced oxidative stress.Aims: The current study purposes to explore the effects of MSCs and FSO on the toxicity of lead acetate on haematological parameters. Material and methods: Forty rats were randomly divided into four groups and 10 rats per group. Group (1): healthy normal group, group (2): rats injected with lead acetate (100 mg/kg b.w.t./intraperitoneal injection) for seven days. group (3): rats were treated with a single dose of bone marrow mesenchymal stem cells (1 × 10 6 cells/rats) intravenously injected after lead intoxication. group (4): rats were given orally flax seeds oil (1ml/kg) for 30 days after lead intoxication. After the experiment, all rats were euthanized and collected blood for the examination of blood parameters. Results: Lead acetate induces a reduction in RBCs, Hb concentration and PCV. Also, lead increases WBCs count, lymphocytes, monocytes, neutrophils, eosinophils, MCV, MCH, MCHC and platelets. Although, MSCs and FSO improved all haematological blood. Conclusions: MSCs and FSO proved to have a powerful effect on the toxicity of lead acetate in the hematopoietic system.

It is well known that, Wound healing in the diabetes is enormous, growing problem and having cost Medicaid. Therefore, this study produces an exciting prospect, to improve diabetic burn healing, and evaluates the ability of a bradykinin potentiating factor (BPF) isolated from scorpion venom (Buthus occitanus) in treatment of burns in diabetic male Guinea pigs. Male Guinea pigs of approximate (550 g body weight each) were divided into five groups. In the normal group; Guinea pigs were interaperitoneally (i.p) injected with 100 µL saline solution. The second, served as control group which were injected with 100 µL saline solution then standard burns were obtained on the dorsal skin. The 3 rd , 4 th and 5 th groups were i.p. injected with (750 mg /kg, body weight) with a single dose of Alloxan, then standard burns were obtained, after that the 4 th group was treated by BPF in 100 µL saline solutions (1μg/gm. b. w.) topically and the 5 th group treated with BPF (1μg/gm. b. w.) topically and interperitonialy (1μg/gm. b. w. twice a week). Every group contains 20 animals and sacrificed at 15 and 30 days post-treatment by BPF (10 animals per each). The results are recorded after monitoring the CBC including (RBCs, WBCs, platelets, Hb content and HCT percentage), lipid profile including (total cholesterol (TC), triglyceride (TGs), high density lipoprotein (HDL), low density lipoprotein (LDL) and very low density lipoprotein (VLDL)) and the skin antioxidant status (catalase activity (CAT) and superoxide dismutase (SOD) and serum nitric oxide (NO) beside malondialdehyde (MDA) concentration were also monitored during the study compared to normal animals. Also serum glucose level was monitored. The recorded results declared that, the treatment with BPF has shown an ameliorative effect on burn healing in diabetic animals. These observations and investigations were the pacemaker for the hypothesized ameliorating activity of BPF in the present study.