Study on Toxic Effects of Lead Acetate on Haematological Parameters of Adult Albino Rats and The Role of Mesenchymal Stem Cells and Flax Seeds Oil as Therapeutic Agents

Abstract

Background: Lead is a toxic heavy metal extensive in the environment. Lead induces oxidative stress. mesenchymal stem cells (MSCs) have multiplied great attention as an efficient tool in regenerative therapy. Flaxseed oil (FSO) natural source of antioxidant agents which reduced oxidative stress.Aims: The current study purposes to explore the effects of MSCs and FSO on the toxicity of lead acetate on haematological parameters. Material and methods: Forty rats were randomly divided into four groups and 10 rats per group. Group (1): healthy normal group, group (2): rats injected with lead acetate (100 mg/kg b.w.t./intraperitoneal injection) for seven days. group (3): rats were treated with a single dose of bone marrow mesenchymal stem cells (1 × 10 6 cells/rats) intravenously injected after lead intoxication. group (4): rats were given orally flax seeds oil (1ml/kg) for 30 days after lead intoxication. After the experiment, all rats were euthanized and collected blood for the examination of blood parameters. Results: Lead acetate induces a reduction in RBCs, Hb concentration and PCV. Also, lead increases WBCs count, lymphocytes, monocytes, neutrophils, eosinophils, MCV, MCH, MCHC and platelets. Although, MSCs and FSO improved all haematological blood. Conclusions: MSCs and FSO proved to have a powerful effect on the toxicity of lead acetate in the hematopoietic system.

The Ameliorative Effects of Lactoferrin Against Lead Acetate Toxicity in Female Albino Rat

“…In line with the present investigation, Peng et al [28] found that lead exposure did not affect hematological parameters. In contrast other research indicated significant changes in different blood indices in response to lead toxicity including anemia, leukocytosis, thrombocytosis, and others [29,30] . These findings suggested that the effect of lead on hematologic parameters is possibly not linear.…”

Secoisolariciresinol diglucoside and anethole ameliorate lipid abnormalities, oxidative injury, hypercholesterolemia, heart, and liver conditions

“…Flavonoid's biological activities are linked to their antioxidant activity via a variety of mechanisms, including “scavenging or quenching free radicals, chelating metal ions, and blocking enzyme systems that produce free radicals” (Gulcin, 2020 ; Nwozo et al, 2023 ). Flaxseed and fennel seeds show excellent antioxidant activity which helps to prevent lipid peroxidation and free radical damage; their extracts may be beneficial in the production of safe food additives (El‐shater et al, 2022 ).…”