Physiologically induced changes in bound phenolics and antioxidants, <scp>DNA</scp>/cytoprotective potentials in pectic poly/oligosaccharides of tomato (<i>Solanum lycopersicum</i>)

Kapoor, Sudhir; Dharmesh, Shylaja M.

doi:10.1002/jsfa.7696

Cited by 13 publications

(10 citation statements)

References 39 publications

(77 reference statements)

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“…Data also showed that Hydnum repandum, Cantharellus clavatus , Lactarius sanguifluus , Sparassis crispa , and Lentius sajor‐caju species also exhibited potent DNA protection, though they contain only moderate levels of antioxidant properties, suggesting that the DNA protective ability of polyphenolic antioxidants need not be due to antioxidative potential but rather could be offering hindrances for free radicals to attach to the DNA molecule. Results support our previous observation, where vanillic acid (VA) and cinnamic acid (CA), having poorer antioxidant potential, exhibited potent DNA protective properties as evidenced by their stronger binding abilities to DNA or proteins 18,27,28 . The current study thus highlights that, as per overall indexing, Termitomyces heimii , Boletus edulis , and Termitomyces mummiformis may be considered as good sources for tyrosinase, antioxidant, and DNA protective potential.…”

Section: Discussionsupporting

confidence: 89%

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Profiling of tyrosinase levels, antioxidant and DNA protective properties in different edible and medicinal mushroom species from India

Hasitha,

Dharmesh,

Urs

et al. 2023

JSFA Reports

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BackgroundTyrosinase is a polyphenol oxidase, one of the globally attracted enzymes for its multi‐mechanistic approaches in various fields such as pharma, food, environmental, medicinal, and agriculture. Tyrosinase from natural nutritive edible mushrooms gained more attention for their rich levels and inexpensive nature. Our previous work has revealed that mushroom tyrosinase has strong similarities in structure and function with that of skin tyrosinase, which has significance as a skin tumour marker. Besides, mushrooms are a richer source of antioxidants. However, it is imperative to analyze all the available varieties of mushrooms for bioactive potentials like tyrosinase and antioxidant levels. Thus, the main goal of the study was to identify the variety of mushroom that can be selected for continuous multiplication and propagation as a viable source for tyrosinase, which can be employed to screen tyrosinase inhibitors as skin tumour inhibitors.ResultsOf the 18 varieties screened for tyrosinase levels, Boletus edulis, Termitomyces hemii, and Termitomyces mummiformis had high levels of tyrosinase and exhibited potent hydrogen peroxide and nitric oxide scavenging activity as well as DNA protection. The potent DNA and antioxidant abilities appear to be due to the enriched levels of hydroxybenzoates.ConclusionThe current study could provide an economic source of tyrosinase (mushrooms) that can be used to monitor tyrosinase inhibitors, a potent component that enables their use against skin cancer.This article is protected by copyright. All rights reserved.

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Section: Discussionsupporting

confidence: 89%

“…DNA protective abilities of different mushroom species were determined electrophoretically using calf thymus DNA on DNA agarose gel 18 . Calf thymus DNA and mushroom extracts (5 μg) were incubated at 37°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Profiling of tyrosinase levels, antioxidant and DNA protective properties in different edible and medicinal mushroom species from India

Hasitha,

Dharmesh,

Urs

et al. 2023

JSFA Reports

Self Cite

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“…Erythrocytes are highly susceptible to attack by reactive oxygen species because of the high amount of polyunsaturated fatty acid content in their membranes and the metal catalyzed oxidation reactions mediated by Fe present in haemoglobin [20] . Hydrogen peroxide generated during the autoxidation of oxyhemoglobin contributes to heme degradation leading to the damage of erythrocytes, which in turn leads to change in whole red blood cell structural conformation and its functioning [18] , [19] , [2] , [10] , [11] , [14] .…”

Section: Resultsmentioning

confidence: 99%

C- Glycosylated flavonoids from black gram husk: Protection against DNA and erythrocytes from oxidative damage and their cytotoxic effect on HeLa cells

2016

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C-Glycosyl flavones are present in different plant tissues and they exhibit health benefits. In the present study, it was found that C-glycosyl flavones are distributed in different milled fractions of black gram and among these fractions, husk had the highest content of C-glycosyl flavones. Two C-glycosyl flavones from black gram husk were extracted and purified by preparative high-performance liquid chromatography (HPLC) column. The purity of each compound was assessed by analytical C18 column. The structure of each compound was confirmed by LC–MS/MS, NMR. The molecular mass of these compounds were found to be [M−H]−, m/z 431.36 and [M−H]−, m/z 431.35 and were identified as vitexin and isovitexin, respectively. Content of vitexin and isovitexin in aqueous ethanol extract was found to be 76 and 65 mg/g of extract, respectively. These C-glycosyl flavones protected DNA and erythrocytes from oxidative damage. The IC50 values for vitexin, isovitexin and quercetin for hemolysis were 6, 5.7 and 2.37 μg, respectively. These compounds also triggered the process of apoptosis in HeLa cells by downregulating Bcl-2 level with the simultaneous upregulation of Bax and caspase-3 protein expression. Thus, C-glycosyl flavones from black gram husk protected DNA and erythrocytes from oxidative damage and exhibited anticancer activity.

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“…Whereas NaOH is widely used in the alkali method, , for instance, the BPs (vanillic, ferulic, p -coumaric, caffeic, syringic, chlorogenic acids, etc.) were successfully extracted from both pectic polysaccharides and pectic oligosaccharides of tomato by 1 M NaOH . Besides, KOH has been also employed to decompose the cell-wall structure .…”

Section: Extraction Methods For Bound Phenolicsmentioning

confidence: 99%

“…were successfully extracted from both pectic polysaccharides and pectic oligosaccharides of tomato by 1 M NaOH. 49 Besides, KOH has been also employed to decompose the cell-wall structure. 50 The alkali method, however, requires a pretreatment process that is rather complex.…”

Section: Extraction Methods For Bound Phenolicsmentioning

confidence: 99%

Review of Distribution, Extraction Methods, and Health Benefits of Bound Phenolics in Food Plants

Wang

Shi-yang

et al. 2020

J. Agric. Food Chem.

180

108

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Phenolic compounds are important functional bioactive substances distributed in various food plants. They have gained wide interest from researchers due to their multiple health benefits. There are two forms of phenolic compounds: free form and bound form. The latter is also called bound phenolics (BPs), which are found mainly in the cell wall and distributed in various tissues/organs of the plant body. They can either chemically bind to macromolecules and food matrixes or be physically entrapped in food matrixes and intact cells. Various isolation methods, including chemical, biological, and physical methods, have been employed to extract BPs from plants. BPs have been shown to have strong biological activities, including antioxidant, probiotic, anticancer, anti-inflammation, antiobesity, and antidiabetic effects as well as beneficial effects on central nervous system diseases. This review summarizes research findings on these topics to help in better understanding of BPs and provide comprehensive information on their health effects.

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Physiologically induced changes in bound phenolics and antioxidants, DNA/cytoprotective potentials in pectic poly/oligosaccharides of tomato (Solanum lycopersicum)

Cited by 13 publications

References 39 publications

Profiling of tyrosinase levels, antioxidant and DNA protective properties in different edible and medicinal mushroom species from India

Profiling of tyrosinase levels, antioxidant and DNA protective properties in different edible and medicinal mushroom species from India

C- Glycosylated flavonoids from black gram husk: Protection against DNA and erythrocytes from oxidative damage and their cytotoxic effect on HeLa cells

Review of Distribution, Extraction Methods, and Health Benefits of Bound Phenolics in Food Plants

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