Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers

Bédard, Charles; Knowles, Roger

doi:10.1128/mr.53.1.68-84.1989

Cited by 425 publications

(85 citation statements)

References 73 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Due to di¡erences in cell size, calculated numbers of type I and type II methanotrophs were roughly similar and shared similar distribution with depth. Since both methanotrophs and ammonia oxidizers contain the PLFAs 16:0 and 16:1g7c [1,32], it is di¤cult to exclude the possibility that ammonia-oxidizing bacteria are incorporating IQ CH R into membrane lipids. The fatty acid 16:1g7c received the most label in this experiment, and this PLFA is common to both type I methanotrophs and ammonia oxidizers.…”

Section: Discussionmentioning

confidence: 99%

“…Ammonia-oxidizing bacteria primarily contain the fatty acids 16:0 and 16:1g7c. These are common fatty acids that are also detected in group I methanotrophs [1].…”

Section: Introductionmentioning

confidence: 90%

“…In addition to their usual source of energy, methanotrophs and ammonia oxidizers also utilize other substrates. Methanotrophs can oxidize ammonium and ammonia oxidizers can oxidize methane, yet neither type of organism can use this energy as the sole source for growth (reviewed by Bedard and Knowles [1]).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Nold

Boschker

Pel

et al. 1999

FEMS Microbiology Ecology

View full text Add to dashboard Cite

To investigate the effect of ammonium addition on the species composition and activity of freshwater methane oxidizing bacteria, intact sediment cores were labeled with IQ CH R and incubated under ambient and elevated ammonium concentrations. After 7 days, methanotroph activity was assessed by quantifying the isotopic composition of the carbon in membrane lipids. The 16-carbon rather than the 18-carbon methanotroph-specific biomarkers showed a clear enrichment in IQ C, suggesting the importance of group I methanotrophs in these sediments. Ammonium addition resulted in a depleted isotopic signal compared to ambient controls, suggesting that high ammonium concentrations inhibit methane incorporation into cellular components. These results compare favorably with studies that showed ammonium inhibition of methane oxidation, and extend these findings by demonstrating the effect of nitrogen fertilization on methanotroph lipid synthesis.

show abstract

Section: Discussionmentioning

confidence: 99%

“…Ammonia-oxidizing bacteria primarily contain the fatty acids 16:0 and 16:1g7c. These are common fatty acids that are also detected in group I methanotrophs [1].…”

Section: Introductionmentioning

confidence: 90%

See 1 more Smart Citation

Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Nold

Boschker

Pel

et al. 1999

FEMS Microbiology Ecology

View full text Add to dashboard Cite

show abstract

“…in rice fields), and the other group contains "high affinity" methanotrophs which are able to make use of the atmospheric CH 4 concentrations (around 1.8 ppm). Ammonium-oxidizing bacteria can also oxidize CH 4 through the enzyme ammonia monooxygenase, which can react with CH 4 instead of NH + 4 (Bédard and Knowles, 1989). The increased use of nitrogen (N) fertilizers, fossil fuel, and cultivation of N-fixing crops have more than doubled the amount of "reactive" nitrogen (N r ) cycling worldwide (Vitousek et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

“…The enzyme methane monooxygenase, which initiates the oxidation pathway of CH 4 , is also able to oxidize NH + 4 . When NH + 4 competes with CH 4 for reactive sites of methane monooxygenase, this can cause inhibition of CH 4 oxidation (Bédard and Knowles, 1989).…”

Section: Introductionmentioning

confidence: 99%

Indications of nitrogen-limited methane uptake in tropical forest soils

Veldkamp¹,

Koehler²,

Corre³

2013

Preprint

View full text Add to dashboard Cite

Tropical forest soils contribute 6.2 Tg yr⁻¹ (28%) to global methane (CH₄) uptake, which is large enough to alter CH₄ accumulation in the atmosphere if significant changes would occur to this sink. Elevated deposition of inorganic nitrogen (N) to temperate forest ecosystems has been shown to reduce CH₄ uptake in forest soils, but almost no information exists from tropical forest soils even though projections show that N deposition will increase substantially in tropical regions. Here we report the results from long-term, ecosystem-scale experiments in which we assessed the impact of chronic N addition on soil CH₄ fluxes from two old-growth forests in Panama: (1) a lowland, moist (2.7 m yr⁻¹ rainfall) forest on clayey Cambisol and Nitisol soils with controls and N-addition plots for 9–12 yr, and (2) a montane, wet (5.5 m yr⁻¹ rainfall) forest on a sandy loam Andosol soil with controls and N-addition plots for 1–4 yr. We measured soil CH₄ fluxes for 4 yr (2006–2009) in 4 replicate plots (40 m × 40 m each) per treatment using vented static chambers (4 chambers per plot). CH₄ fluxes from the lowland control plots and the montane control plots did not differ from their respective N-addition plots. In the lowland forest, chronic N addition did not lead to inhibition of CH₄ uptake; instead, a negative correlation of CH₄ fluxes with nitrate (NO₃⁻) concentrations in the mineral soil suggests that increased NO₃⁻ levels in N-addition plots had stimulated CH₄ consumption and/or reduced CH₄ production. In the montane forest, chronic N addition also showed negative correlation of CH₄ fluxes with ammonium concentrations in the organic layer, which suggests that CH₄ consumption was N limited. We propose the following reasons why such N-stimulated CH₄ consumption did not lead to statistically significant CH₄ uptake: (1) for the lowland forest, this was caused by limitation of CH₄ diffusion from the atmosphere into the clayey soils, particularly during the wet season, as indicated by the strong positive correlations between CH₄ fluxes and water-filled pore space (WFPS); (2) for the montane forest, this was caused by the high WFPS in the mineral soil throughout the year, which may not only limit CH₄ diffusion from the atmosphere into the soil but also favour CH₄ production; and (3) both forest soils showed large spatial and temporal variations of CH₄ fluxes. We conclude that in these extremely different tropical forest ecosystems there were indications of N limitation on CH₄ uptake. Based on these findings, it is unlikely that elevated N deposition on tropical forests will lead to widespread inhibition of CH₄ uptake

show abstract

Cometabolism of chlorinated solvents by nitrifying bacteria: Kinetics, substrate interactions, toxicity effects, and bacterial response

Ely

Williamson

Hyman

et al. 1997

Biotechnol. Bioeng.

View full text Add to dashboard Cite

Pure cultures of ammonia‐oxidizing bacteria, Nitrosomonas europaea, were exposed to trichloroethylene (TCE), 1,1‐dichloroethylene (1,1‐DCE), chloroform (CF), 1,2‐dichloroethane (1,2‐DCA), or carbon tetrachloride (CT), in the presence of ammonia, in a quasi‐steady‐state bioreactor. Estimates of enzyme kinetics constants, solvent inactivation constants, and culture recovery constants were obtained by simultaneously fitting three model curves to experimental data using nonlinear optimization techniques and an enzyme kinetics model, referred to as the inhibition, inactivation, and recovery (IIR) model, that accounts for inhibition of ammonia oxidation by the solvent, enzyme inactivation by solvent product toxicity, and respondent synthesis of new enzyme (recovery). Results showed relative enzyme affinities for ammonia monooxygenase (AMO) of 1,1‐DCE ≈ TCE > CT > NH3 > CF > 1,2‐DCA. Relative maximum specific substrate transformation rates were NH3 > 1,2‐DCA > CF > TCE ≈ 1,1‐DCE > CT (=0). The TCE, CF, and 1,1‐DCE inactivated the cells, with 1,1‐DCE being about three times more potent than TCE or CF. Under the conditions of these experiments, inactivating injuries caused by TCE and 1,1‐DCE appeared limited primarily to the AMO enzyme, but injuries caused by CF appeared to be more generalized. The CT was not oxidized by N. europaea while 1,2‐DCA was oxidized quite readily and showed no inactivation effects. Recovery capabilities were demonstrated with all solvents except CF. A method for estimating protein yield, the relationship between the transformation capacity model and the IIR model, and a condition necessary for sustainable cometabolic treatment of inactivating substrates are presented. © 1997 John Wiley & Sons, Inc. Biotechnol Bioeng 54: 520–534, 1997.

show abstract

Physiology, biochemistry, and specific inhibitors of CH4, NH4+, and CO oxidation by methanotrophs and nitrifiers

Cited by 425 publications

References 73 publications

Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Ammonium addition inhibits 13C-methane incorporation into methanotroph membrane lipids in a freshwater sediment

Indications of nitrogen-limited methane uptake in tropical forest soils

Cometabolism of chlorinated solvents by nitrifying bacteria: Kinetics, substrate interactions, toxicity effects, and bacterial response

Contact Info

Product

Resources

About