Phytase production by Candida melibiosica 2491 alkalophylic strain

Abstract

It has been proved that Candida melibiosica 2491 yeast strain, an intracellular phytase producer, can be well adapted to a nutritious medium with a pH range from 3.0 to 9.0. The best development of the strain was achieved at pH 8.0, which refers to the rarely encountered alkalophylic yeast strains. The maximum biosynthesis was obtained in a medium with pH of 8.5, 24th-hour aged inoculum added at concentration of 6.6% (v/v) with optical density OD600 of 0.68, aeration degree 500 dm 3 /dm 3 .h, stirring speed 300 rpm, and 24h duration of the cultivation in bioreactor. The optimal phytase activity was reached at 60°С and рН 4.5. However, over 80% of the maximum enzymatic activity was observed at the pH interval ranging from 4.0 to 8.5, which provides great potential for practical applications.

Citrobacter amalonaticus Phytase on the Cell Surface of Pichia pastoris Exhibits High pH Stability as a Promising Potential Feed Supplement

“…3A . The optimal temperature for cell surface phytase and secreted phytase is 60°C, which is similar to data reported for phytases from Candida melibiosica 2491 [37] and Pichia anomala [38] . Phytase expressed on the surface of yeast cells and its secreted counterparts were stable when incubated at 60–70°C.…”

Stable current outputs and phytate degradation by yeast-based biofuel cell

“…We have established that C. melibiosica 2491 is able to transfer electrons extracellularly through both mechanisms, direct and indirect, according to the continuance of the cultivation process (batch or semi‐batch systems) (Babanova et al ., ; Hubenova et al ., ). At the same time, it was reported that at normal cultivation (without polarization) C. melibiosica 2491 expressed high phytase activity (Georgiev et al ., ).…”