Phytochemical Analysis and Antifungal Activity of Aqueous Leaf Extract of Trema guineensis (Ulmaceae), A Plant from the Ivoirian Pharmacopoeia

Abstract

Trema guineensis is a woody plant distributed in tropical forest of which leaf and bark extracts are traditionally used for the treatment of various diseases including fever, bronchitis, and gastrointestinal disorders. Previous studies have highlighted their antibacterial activity. So, present work was designed to examine the phytochemical and antifungal properties of aqueous crude extract of T. guineensis leaves. The bioactive components extracted from leaves were tested against pathogenic fungi using the agar tube dilution method. Antifungal activity of aqueous leaves extracts was carried out against selected pathogenic fungal strains as Aspergillus fumigatus, Cryptococcus neoformans and Candida albicans. The phytochemical analysis of the aqueous crude extract revealed the presence of secondary metabolites widely reported as antifungal such as flavonoids, saponins, quinones, alkaloids, polyphenols. The results showed that the aqueous crude leaves extract of T. guineensis was effective in inhibiting the fungal growth and were active against A. fumigatus, C. neoformans and C. albicans with MIC and MFC ranged from 20 to 200 mg/mL and 100 to 400 mg/mL, respectively. The efficient antifungal activity of T. guineensis from the present investigation revealed that aqueous leaf crude extract of the selected plant had a moderate potential to inhibit the growth of pathogenic fungal strains. This finding showed that the aqueous extract of T. guineensis exerted an antifungal effect on C. albicans, A. fumigatus and C. neoformans and supports its traditional use in herbal medicine.

Secondary metabolites of Diaporthe cameroonensis, isolated from the Cameroonian medicinal plant Trema guineensis

“…Regarding the potent talents of Diaporthe, we are on the quest to the exploration of structure-activity relationships of cytochalasins to establish their trends for various medical applications [5]. Along the same lines, we herein report the isolation and structural elucidation of a new polyketide 1 and a new acetylated alternariol derivative 2 as well as fifteen known compounds including three alternariol derivatives 3-5, one chromone 6, one biphenyl 7, seven cytochalasins 8-14, two cytosporones 15 and 16, and one macrolide 17 from the endophytic fungus, Diaporthe cameroonensis, isolated from the root of Trema guineensis, a Cameroonian medicinal plant from the family Ulmaceae [11].…”

The Potential of Katuk Hutan (Phylanthus reticulus) as a Medicinal Plant

“…Furthermore, it is said that the combined form of the four extracts is traditionally used to treat diarrhoea because it is believed that each of them supports its active component for optimal efficacy [30]. Meanwhile, the medicinal plant Trema guineensis, which contain alkaloid compounds, tannins, saponins, flavonoids and anthraquinones, can inhibit S. aureus, E. coli, and S. enteritidis bacteria with a zone of inhibition [18]. The ethanol extract of Origanum elongatum leaves was most effective against Escherichia coli with an inhibition zone diameter of 30.33 mm.…”

Secondary metabolites of Diaporthe sp., isolated from the Cameroonian medicinal plant Trema guineensis (Schumach. & Thonn.) Ficalho

“…Regarding the potent talents of Diaporthe, we are on the quest to the exploration of structure-activity relationships of cytochalasins to establish their trends for various medical applications [5]. Along the same lines, we herein report the isolation and structural elucidation of a new polyketide (1) and a new acetylated alternariol derivative (2) as well as fifteen known compounds including three alternariol derivatives (3)(4)(5), one chromone (6), one biphenyl (7), seven cytochalasins (8)(9)(10)(11)(12)(13)(14), two cytosporones (15)(16), and one macrolide (17) from the endophytic fungus, Diaporthe sp. isolated from the root of Trema guineensis, a Cameroonian medicinal plant from the family Ulmaceae [11].…”