Phytochemical analysis and biological evaluation of the aerial parts from Symphytum anatolicum Boiss. and Cynoglottis barrelieri (All.) Vural &amp; Kit Tan (Boraginaceae)

Varvouni, Elisavet-Foteini; Zengin, Gökhan; Graikou, Konstantia; Ganos, Christos; Mroczek, Tomasz; Chinou, İoanna

doi:10.1016/j.bse.2020.104128

Cited by 21 publications

(37 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The TAP in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The TAP in M. maritima shoots extract was also larger (41.98 mg GAE/g of extract) than in calli (9.20 GAE/g of extract), in vivo leaves (16.06 GAE/g of extract), stems (14.55 GAE/g of extract), inflorescences (21.70 GAE/g of extract) and roots (3.17 GAE/g of extract) of Heliotropium indicum [ 23 ], flowers (18.43 GAE/g of extract) and roots (13.11 GAE/g of extract) of Cynoglossum creticum [ 24 ], and aerial parts (32.7 GAE/g of extract) [ 25 ] and roots (11.45 GAE/g of extract) of Symphytum anatolicum [ 26 ]. The TAP in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The TAP in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. However, TAP in M. maritima shoot tissues was lower (1.76 mg RE/g of extract) compared to in vivo leaves (4.39 RE/g of extract) and flowers (21.77 RE/g of extract) of C. creticum [ 24 ], aerial parts (20.9 RE/g of extract) of Cynoglottis barrelieri [ 25 ], leaves (3.32 QE/g of extract), stems (3.23 QE/g of extract) and inflorescences (4.90 QE/g of extract) of H. indicum [ 23 ], and aerial parts (13.3 RE/g of extract) [ 25 ] and roots (2.74 RE/g of extract) of S. anatolicum [ 26 ]. Folin and Ciocalteu method, one of the most popular, is simple and requires inexpensive reagents.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, enzyme inhibitors from natural sources are needed to replace these synthetic ones. In recent investigations, several researchers reported enzyme inhibition properties of several Boraginaceae members such as, Alkanna sfikasiana [ 49 ], Cynoglossum creticum [ 24 ], Cynoglottis barrelieri [ 25 ], Echium confusum [ 50 ], Onosma aucheriana , Onosma sieheana , Onosma frutescens , Onosma stenoloba , and Onosma sericea [ 51 , 52 ] Symphytum anatolicum [ 25 , 26 ]. In the present study, the enzyme inhibitory effects of M. maritima extracts were investigated using different enzymes.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Song

Sivanesan

et al. 2020

Plants

View full text Add to dashboard Cite

Mertensia maritima (L.) Gray is threatened with extinction owing to climate change, poor seed germination, and ocean warming. In vitro explant-culture is used for ex situ preservation and plantlet massive production. In vitro cell and organ cultures serve as an alternative plant material source to investigate the biological activities and phytochemical profiles of rare plants. We aimed to develop an efficient callus and shoot production protocol and investigate bioactive metabolites, antioxidants, and enzyme inhibitory potential of M. maritima calli, shoots, and in vivo seedlings. The effects of combinations of different plant growth regulators, 6-BA (N6-benzyladenine), 6-KN (Kinetin), TDZ (Thidiazuron), and NAA (1-Naphthylacetic acid), in MS (Murashige and Skoog) nutrient medium were studied. The highest callus proliferation was obtained after 5-week cultivation over a 16-h photoperiod on growth medium MS enriched with 4 µM each of 6-BA and NAA. The medium with 2 µM 6-BA and 4 µM 6-KN had the best shoot induction rate (91.1%) with a mean of 13.4 shoots. The combination of two cytokinins (6-BA and 6-KN) was found to be effective in M. maritima shoot regeneration. The rooting frequency was 100% in ½ MS with Indole-3-butyric acid (IBA 2 µM). The number of detected compounds and chemical composition in the M. maritima shoots and seedlings extracts were similar. The total amount of phenolics in the shoots was 216.4% and 369.5% higher than in seedlings and calli, respectively. The total amount of flavonoids in the shoots was 241.1% and 429.3% higher than in seedlings and calli, respectively. The best antioxidant activity was obtained in the shoots, followed by seedlings and calli. However, the order was seedlings > calli > shoots regarding metal chelating ability. The strongest acetylcholinesterase inhibition properties were obtained in the calli, followed by seedlings and shoots. However, the tested samples can be ranked as seedlings > shoots > calli in butylcholinestrase inhibition assay. This study is the first report on the enzyme inhibitory effects of M. maritima extracts, providing valuable contributions to the scientific community.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Song

Sivanesan

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…Total antioxidant activity of the seed extracts was determined using the phosphomolybdenum method, according to the literature [38]. Total antioxidant capacity is expressed as TE as determined by the equation of the standard Trolox curve.…”

Section: Total Antioxidant Activitymentioning

confidence: 99%

“…Radical scavenging activity of the extracts were estimated against DPPH and ABTS. These methods are based on the decolourisation of free radicals by the plant sample [38], and the activity is expressed as TE.…”

Section: Radical Scavenging Activitymentioning

confidence: 99%

Chemical Characterisation-Biological Evaluation of Greek Cultivar Cardoon Seeds (Cynara cardunculus). A By-product with Potential High Added Value

et al. 2021

Self Cite

View full text Add to dashboard Cite

Cynara cardunculus (artichoke) is a perennial plant of the Mediterranean basin, known since antiquity as food and for its therapeutic properties. Cynara is a relatively small genus with two cultivated species and one wild one. Recently, successful efforts have been made to cultivate wild cardoon and monetise it as a bioenergy crop. In this study, the seeds of an established Greek cultivar of C. cardunculus, cultivated in the experimental field and used as biofuel, have been researched for their chemical profile and nutritional value. According to the results, six lignans were isolated [arctigenin, arctiin, trachelogenin, tracheloside, cynarinine, and ethylate of trachelogenin (isolated for the first time from a natural source)] as well as the most characteristic metabolites of the genus (linoleic acid, trilinolein, and 3,5-dicaffeoylquinic acid). Moreover, the total phenolic content (31.18 – 54.51 mg gallic acid equivalents/g extract) and antioxidant and enzyme inhibitory activities of the seeds have been evaluated and showed strong antioxidant properties (44.42 – 516.81 mg gallic acid equivalents/g extract) as well as satisfactory bleaching (enzyme tyrosinase, 16.95 – 23.80 mg kojic acid equivalents/g extract), antidiabetic (enzymes a-amylase, a-glucosidase, 0.14 – 1.75 mmol acarbose equivalents/g extract), and protective against neurodegenerative disease (cholinesterase enzymes, 0.49 – 1.22 mg galanthamine equivalents/g extract) activities. The nutritional evaluation of the seeds confirmed them as a rich source of unsaturated fatty acids, dietary fibre (24.1%), and high protein content (19.3%). It is noteworthy that such a neglected bioactive by-product, with essentially high nutritional value, as the studied seeds could be investigated for its value-added applications towards food and food supplements areas.

show abstract

Phytochemical profiling and bioactivity assessment of underutilized Symphytum species in comparison with Symphytum officinale

Luca,

Zengin,

Kulinowski

et al. 2024

J Sci Food Agric

View full text Add to dashboard Cite

BACKGROUNDSymphytum (comfrey) genus, particularly Symphytum officinale, has been empirically used in folk medicine mainly for its potent anti‐inflammatory properties. In an attempt to shed light on the valorization of less known taxa, the current study evaluated the metabolite profile and antioxidant and enzyme inhibitory effects of nine Symphytum species.RESULTSPhenolic acids, flavonoids and pyrrolizidine alkaloids were the most representative compounds in all comfrey samples. Hierarchical cluster analysis revealed that, within the roots, S. grandiflorum was slightly different from S. ibericum, S. caucasicum and the remaining species. Within the aerial parts, S. caucasicum and S. asperum differed from the other samples. All Symphytum species showed good antioxidant and enzyme inhibitory activities, as evaluated in DPPH (up to 50.17 mg Trolox equivalents (TE) g−1), ABTS (up to 49.92 mg TE g−1), cupric reducing antioxidant capacity (CUPRAC, up to 92.93 mg TE g−1), ferric reducing antioxidant power (FRAP, up to 53.63 mg TE g−1), acetylcholinesterase (AChE, up to 0.52 mg galanthamine equivalents (GALAE) g−1), butyrylcholinesterase (BChE, up to 0.96 mg GALAE g−1), tyrosinase (up to 13.58 mg kojic acid equivalents g−1) and glucosidase (up to 0.28 mmol acarbose equivalents g−1) tests. Pearson correlation analysis revealed potential links between danshensu and ABTS/FRAP/CUPRAC, quercetin‐O‐hexoside and DPPH/CUPRAC, or rabdosiin and anti‐BChE activity.CONCLUSIONSBy assessing for the first time in a comparative manner the phytochemical–biological profile of a considerably high number of Symphytum samples, this study unveils the potential use of less common comfrey species as novel phytopharmaceutical or agricultural raw materials. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

show abstract

Phytochemical analysis and biological evaluation of the aerial parts from Symphytum anatolicum Boiss. and Cynoglottis barrelieri (All.) Vural & Kit Tan (Boraginaceae)

Cited by 21 publications

References 32 publications

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Screening of Bioactive Metabolites and Biological Activities of Calli, Shoots, and Seedlings of Mertensia maritima (L.) Gray

Chemical Characterisation-Biological Evaluation of Greek Cultivar Cardoon Seeds (Cynara cardunculus). A By-product with Potential High Added Value

Phytochemical profiling and bioactivity assessment of underutilized Symphytum species in comparison with Symphytum officinale

Contact Info

Product

Resources

About