Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits

Andriani, Yosie; Ramli, Nadiah Madihah; Syamsumir, Desy Fitrya; Kassim, Murni Nur Islamiah; Jaafar, Jasmin; Aziz, Nur Asniza; Marlina, Leni; Musa, Neksumi; Mohamad, Habsah

doi:10.1016/j.arabjc.2015.11.003

Cited by 69 publications

(44 citation statements)

References 37 publications

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“…Different studies reported that the growth of the several fungi was controlled using the extract of natural plant parts (Andriani et al 2015;Javadian et al 2016;ShamsGhahfarokhi et al 2006). The present study revealed the mycelium growth of the fungal strain was limitedly inhibited only by Pandanus odoratissimus among five plant extracts used in the experiment.It was also found that 6% NaCl was able to inhibit ̴ 55% fungal growth, which can be a great promising factor for successful future control practice.…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

2017

Int. J. Biosci.

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Mango, 'the king of the fruits' is one of the most popular, nutritionally rich fruit in Bangladesh. One of the limiting factors that influence the economic value of mango is its short storage life at ambient temperature due to spoilage, caused by microbial invasion. Fungi are the primary spoiling organism as they are rich in cell wall degrading enzymes. Current study was carried out to isolate, characterize and to find out a cost effective control measure for the fungal strains associated with post-harvest spoilage of mango. One fungal strain was isolated from the spoiled ''Totapuri' mango. The primary characterization of the fungal strain shows that the colony diameter, culture characteristics and sporulation of the fungus is greatly influenced by the type of growth medium used. CDA exhibited higher mycelial growth, whereas the PDA culture medium was responsible for heavy sporulation. Molecular confirmation of the isolate as fungus was done by the extraction of genomic DNA and ITS region amplification by PCR. The fungi grew optimally at 25°C, pH 9 and in dextrose carbohydrate source. The fungus exhibited high sensitivity to citric acid and NaCl.. The results of this investigation combined with further studies like ITS region sequencing, can be used in precise identification of organism causing mango spoilage and it will also be possible to decipher a biological control mechanism for this fungus.

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Section: Discussionmentioning

confidence: 99%

Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

2017

Int. J. Biosci.

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“…After 72 hrs, colorimetric MTT method assay was used to measure the living cells (slightly modified from Andriani et al [11]). The MTT was dissolved at 5 mg/ml in phosphate-buffered saline.…”

Section: -(45-dimethylthiazol-2-yl) 25-diphenyl Tetrazoliumbromidementioning

confidence: 99%

Anti-Inflammatory Activity of Bacteria Associated With Marine Sponge (Haliclona Amboinensis) via Reducting No Production and Inhibiting Cyclooxygenase-1, Cyclooxygenase-2, and Secretory Phospholipase A2 Activities

Andriani

Marlina

Mohamad

et al. 2017

Asian J Pharm Clin Res

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Objective: This study aimed to investigate the anti-inflammatory activity of methanol extract and fractions of bacteria associated with sponge (Haliclona amboinensis) and to evaluate their effect in reducing NO production and inhibiting cyclooxygenase-1 (COX-1), cyclooxgenase-2 (COX-2) and secretory phospholipase A2 (sPLA2) activity.Methods: All bacterial isolates were cultured and supernatants were collected for the extraction of secondary metabolites using diaion HP-20 to obtain methanol extracts. Evaluation of cytotoxicity property was carried out on macrophage cell lines (RAW264.7) by 3-(4,5-dimethylthiazol-2-yl) 2,5-diphenyl tetrazoliumbromide assay. Anti-inflammatory screening was done by inducible nitric oxide assay on RAW264.7 cell lines with lipopolysaccharide (LPS) stimulation. Dianion HP-20 was used to remove salt content. A selected methanol extract was subjected to further fractionations by C-18 reverse phase and their anti-inflammatory potential was evaluated by COX-1 and COX-2, and sPLA2 enzymatic assay.Results: Seven methanol extracts showed no cytotoxic property against RAW 264.7 cell line (inhibitory concentration 50% > 30 µg/ml) and selected for anti-inflammatory screening assay. Result showed methanol extract HM 1.2 reduced NO production >80% and it has been selected for phytochemical screening, further fractionations and assay. Phytochemical screening showed alkaloids and terpenoids present in the HM 1.2. The HM 1.2 and its fractions (F1, F2, F1C1, F1C2, F1C3, and F1C4) were proven to inhibit COX-1, COX-2, and sPLA2 activity in the range of 60.516-116.886%, 20.554-116.457%, and 70.2667-114.8148%, respectively. Conclusions:This study revealed that bacteria associated with H. amboinensis have produced anti-inflammatory activity via reducing NO production and inhibiting COX-1, COX-2, and sPLA2 activity.

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“…[9] Phytochemical Screening Phytochemical analysis of phenolics, tannins, flavonoids, sterol and triterpenes, alkaloids, saponins, and anthraquinone glycoside was carried out using standard procedures. [10][11][12] Water Extract About 1 g of date seed powder was dissolved in 14 ml of distilled water and was boiled gently using mantle, then the extract was filtered using Whatman filter paper.…”

Section: Preparation Of Seed Extract Of Datesmentioning

confidence: 99%

Untitled

Ravi

2017

IJGP

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Aim: This study was carried out to investigate the anticancer and antibacterial activity of date seeds and its phytochemical composition. Materials and Methods: Qualitative phytochemical screening was performed using date seed powder using biochemical assays. Gas chromatography-mass spectrometry (GC-MS) analysis was performed to identify the phytochemical contents in the extract. MTT assay was carried out to study the cytotoxicity of the seed extracts against HCT-15 (human coloretal cancer) cells. Antibacterial activity was studied using agar well diffusion method, against Bacillus cereus and Escherichia coli. Results: GC-MS analysis of acetone and chloroform extract of date seeds suggests that acetone extract contains majorly of aliphatic molecules and chloroform extract contains aromatic molecules. Cytotoxicity study showed that acetone extract is highly cytotoxic with inhibitor concentration 50 (IC50) value at 20 µg/ml and chloroform extract is moderately cytotoxic with IC50 value at 100 µg/ml concentration against HCT-15 cells. Antibacterial study showed that chloroform extract had no antagonistic activity against bacteria, whereas acetone extract demonstrated significant antibacterial activity with a zone of inhibition of 17 mm and 20 mm against B. cereus and E. coli, respectively at 1 mg/ml concentration. Conclusion: Results of this study conclude that acetone extract of date seeds (Phoenix dactylifera L) contains significant potential for pharmaceutical applications, in the field of antibacterial and anticancer drug discovery.

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Phytochemical analysis, antioxidant, antibacterial and cytotoxicity properties of keys and cores part of Pandanus tectorius fruits

Cited by 69 publications

References 37 publications

Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

Isolation, characterization and control of a fungus responsible for post-harvest mango spoilage from northern region of Bangladesh

Anti-Inflammatory Activity of Bacteria Associated With Marine Sponge (Haliclona Amboinensis) via Reducting No Production and Inhibiting Cyclooxygenase-1, Cyclooxygenase-2, and Secretory Phospholipase A2 Activities

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