Phytochemical Analysis of Symphytum officinale Root Culture Extract

Le, Violeta; Dolganyuk, Vyacheslav; Сухих, А. С.; Babich, Olga; Иванова, Светлана; Prosekov, Alexander; Dyshlyuk, Lyubov

doi:10.3390/app11104478

Cited by 16 publications

(7 citation statements)

References 52 publications

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“…The literature indicates the potential therapeutic effects of Symphytum officinale . The plant has been reported to possess anti-inflammatory, anti-apoptotic, antitumor, neuroprotective, and antioxidant properties [ 335 ]. Furthermore, comfrey has been shown to facilitate bone regeneration [ 336 ].…”

Section: Introductionmentioning

confidence: 99%

Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

et al. 2023

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The skin shows the physiological condition of the body’s organs and systems that prevent infections and physical damage. Throughout the ages, in folk medicine, phytotherapy was considered a primary form of treatment in all countries, including Kazakhstan, due to the abundance and availability of plant-based remedies. This paper discusses several medicinal plants that are traditionally used in the treatment of skin diseases in the Republic of Kazakhstan. The chemical composition of these plants was analyzed, with a particular focus on the biologically active basic compounds responsible for their therapeutic efficiency in treating skin ailments.

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Section: Introductionmentioning

confidence: 99%

Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

et al. 2023

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“…и этических проблем актуален альтернативный способ получения данного углеводорода, например, с использованием биотехнологических методов in vitro [1,20,29]. В ранее опубликованной работе авторов установлено, что экстракт корневой культуры in vitro окопника лекарственного (Symphytum officinale) содержит значительное количество сквалена [10]. В работе S. Potijun [21] в качестве продуцента сквалена использовалась микроводоросль Chlamydomonas reinhardtii.…”

Section: Introductionunclassified

Evaluation of the Geroprotective Effect of Squalene on the Caenorhabditis Elegans Model

Vesnina

Dolganyuk

Dmitrieva

et al. 2022

SJLSA

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Background. Increasing healthy life expectancy is a priority in modern healthcare. Squalene, a triterpene exhibiting antioxidant, anti-inflammatory and other properties, is a promising candidate for geroprotectors. Purpose. Assess the activity of squalene, that is, the possibility of its further use as a geroprotector. Materials and methods. Squalene was purchased from Sigma-Aldrich (USA) for research. Squalene solutions were prepared with concentrations of 10, 50, 100, 200, 400, 800, and 1600 µmol/L (solvent dimethyl sulfoxide—DMSO). The work was carried out on the basis of the Laboratory for Biotesting of Natural Nutraceuticals (KemGU, Russia). Antioxidant activity was assessed by capturing ABTS radical cations in comparison with the activity of ascorbic acid. The model organism Caenorhabditis elegans N2 Bristol was purchased from the Laboratory for the Development of Innovative Medicines and Agrobiotechnologies of the Moscow Institute of Physics and Technology. To assess the stress resistance of nematodes during cultivation, conditions of thermal (33°C) and oxidative (adding 15 μl of 1 M paraquat, 20°C) stress were created for 24 and 48 h. conditions (20 °C) for 61 days. The statistical significance of the results was assessed using Student’s t-test (in Microsoft Office Excel 2007). Lifespan data were assessed using a Kaplan-Meier survival plot combined with a log-rank test (Mantel-Cox). Data processing was carried out using the OASIS online application (https://sbi.postech.ac.kr/oasis/). Results. The results showed that squalene solutions did not exhibit antioxidant properties; did not trap radical cations with ABTS. A solution of squalene at a concentration of 10 μM contributed to an increase in the stress resistance of nematodes. Under conditions of oxidative stress, the survival of nematodes during cultivation for 24 hours is 3.1% higher than in the control. Under conditions of heat stress during cultivation for 48 hours, the survival rate is higher by 11.3%. Squalene solutions did not affect the lifespan of nematodes. Conclusion. Presumably, the antioxidant activity of squalene is expressed not in the capture of free radicals, but in the ability to act on biomolecules that lead to oxidative stress. A squalene solution with a concentration of 10 μM can reduce the negative impact of one of the factors causing premature aging. In order to assess the possibility of using squalene as a geroprotector without relying on cation-radical capture, further studies are needed to study the impact of the substance on other targets, mechanisms of action related to the occurrence of age-associated diseases. For example, analysis of the effect of squalene solutions on the growth rate of C. elegans larvae, the ability to influence the expression of antioxidant defense genes, the presence of anti-inflammatory and other properties.

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“…The biological variables that affect the therapy's effectiveness include the temperature and surface area of the skin as well as local blood flow. 5 In this study, 126 patients with osteoarthritis of the knee joints underwent iontophoresis with newly developed gel containing symphytum officinale & indomethacin gel, followed by an exercise protocol. The patients' pain, physical function, sleep quality, and gait patterns were all evaluated.…”

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confidence: 99%

“…Symphytum officinale gel is a safe and effective substitute for NSAIDs due to its unique properties. 5…”

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confidence: 99%

Evaluation Of Novel Iontophoretic Gel Containing Symphytum Officinale On Knee Osteoarthritic Patients

Sharmaa,

Tonkb,

Malik

et al. 2024

jaz

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Introduction: One of the most prevalent disorders causing pain in the musculoskeletal system and eventual disability is osteoarthritis (OA). The concurrent use of pharmaceutical and non-pharmacological therapies is an essential component of the therapy. The purpose of this study was to assess the efficacy of iontophoresis therapy in patients with osteoarthritis (OA) of the knee joints using a newly formulated Gel containing symphytum officinale. Material and Methods:The study included 126 patients with Knee osteoarthritis treated at the Outpatient department at Delhi Pharmaceutical Sciences & Research University, New Delhi. Three groups were randomly selected: group I (n = 42), received iontophoresis with newly developed Gel containing symphytum officinale treatment was applied followed by exercise protocol, group II (n = 42) received iontophoresis with Indomethacin gel was applied followed by exercise protocol , and group III (n = 42) received placebo treatment with knee exercise protocol only. The Visual analog scale (VAS), Western Ontario and McMaster Universities Arthritis Index (WOMAC), Pittsburg sleep quality index (PSQI) & Walking cadence were used for the clinical evaluation of the patients. Results: Patients treated with a new gel containing symphytum officinale showed significant improvement in all assessed parameters compared to those treated with Indomethacin gel and placebo. Conclusions: The most favorable iontophoresis resulted from the introduction of a new gel containing symphytum officinale, followed by an exercise protocol.

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Phytochemical Analysis of Symphytum officinale Root Culture Extract

Cited by 16 publications

References 52 publications

Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

Medicinal Plants of the Flora of Kazakhstan Used in the Treatment of Skin Diseases

Evaluation of the Geroprotective Effect of Squalene on the Caenorhabditis Elegans Model

Evaluation Of Novel Iontophoretic Gel Containing Symphytum Officinale On Knee Osteoarthritic Patients

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