Phytochemical and antitrypanosomal investigation of the fractions and compounds isolated fromArtemisia elegantissima

Abstract

The ethanol extract of the aerial parts of A. elegantissima was fractionated by column and preparative thin-layer chromatography into six fractions (A-F) yielding 13 compounds, these were evaluated for their antitrypanosomal activity against T.b. brucei at different concentrations.Results: Thirteen compounds were isolated from A. elegantissima: (Z)-p-hydroxy cinnamic acid, stigmasterol, b-sitosterol, betulinic acid, bis-dracunculin, dracunculin, scopoletin, apigenin, dihydroluteolin, scoparol, nepetin, bonanzin, and 3 0 ,4 0 -dihydroxy bonanzin. The fractions D-F were found to be active at the concentration of 20 mg/ml and three compounds isolated from these fractions, scopoletin (MIC 0.19 mg/ml), 3 0 ,4 0 -dihydroxy bonanzin (MIC ¼ 6.25 mg/ml) and bonanzin (MIC ¼ 20 mg/ml), were found to be highly active. Discussion and conclusion: Artemisia elegantissima was phytochemically and biologically explored for its antitrypanosomal potential against T.b. brucei. The number and orientation of phenolic hydroxyl groups play an important role in the antitrypanosomal potential of coumarins and flavonoids. The compounds 3 0 ,4 0 -dihydroxy bonanzin and scopoletin with low MIC values, hold potential for use as antitrypanosomal drug leads.

Effect of Temperature on 1H NMR Spectra, Antitrypanosomal Activity, Conformational Analysis, and Molecular Docking of Curine Derivatives from Berberis brevissima

“…The antitrypanosomal assay , was performed on the bloodstream form (strain 427) of T.b. brucei obtained from Ugandan sheep.…”

Φυτοχημική μελέτη και έλεγχος βιολογικής δράσης του είδους Staehelina uniflosculosa Sibth. & Sm. της οικογένειας Asteraceae

“…Άλλα φυτά της οικογένειας Asteraceae, από τα οποία έχει απομονωθεί η νεπετίνη, είναι το Santolina chamaecyparissus (Sala. et al, 2000), το Tithonia diversifolia (Zhao et al, 2010), το Arctotis arctotoides (Nasim and Afolayan, 2007) το Cirsium oligophyllum (Uehara et al, 2012), το Crossostephium chinese (Uehara et al, 2014), το Artemisia elegantissima (Rashid et al, 2014), το Anvillea radiata (Destandau et al, 2015) ενώ από το γένος Inula, έχει βρεθεί στα φυτά Ι. britannica (Kim et al, 2002) και I. viscosa (Talib et al, 2012).…”

“…Παθογόνοι μικροοργανισμοί που αναφέρεται ότι αναστέλλονται από αυτή, είναι το Escherichia coli (Alvarez et al, 2007, Talib et al, 2012, καθώς και τα βακτήρια Bacillus subtilis, Staphylococcus aureus και Shigella sonnei (Nasim and Afolayan, 2007). Στη δράση αυτή συγκαταλέγεται και η αναστολή που προκαλεί το φυτό Artemisia elegantissima στο Trypanosoma brucei, το οποίο επιφέρει θάνατο στα βοοειδή (Rashid et al, 2014).…”