Phytochemical and bioactivity evaluation of secondary metabolites and essential oils of Sedum rubens growing wild in Jordan

Odeh, Ammar A.; Al‐Jaber, Hala I.; Barhoumi, Lina M.; Al-Fawares, O’la; Shakya, Ashok K.; Al–Qudah, Mahmoud A.; Sanabra, Ola

doi:10.1016/j.arabjc.2023.104712

Cited by 6 publications

(3 citation statements)

References 56 publications

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“…Essential oils (EOs) were extracted from fresh inflorescence heads of E. polyceras at different growth stages and according to the procedure described in the literature [ 27 , 28 ]. Briefly, a 300 g sample of fresh inflorescence heads collected at each flowering stage was coarsely powdered and then subjected to hydro-distillation for 3 h in a Clevenger-type apparatus.…”

Section: Methodsmentioning

confidence: 99%

Exploring Echinops polyceras Boiss. from Jordan: Essential Oil Composition, COX, Protein Denaturation Inhibitory Power and Antimicrobial Activity of the Alcoholic Extract

Hasan,

Shakya,

Al-Jaber

et al. 2023

Molecules

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In this article, we present the first detailed analysis of the hydro-distilled essential oil (HDEO) of the inflorescence heads of Echinops polyceras Boiss. (Asteraceae) from the flora of Jordan, offering observations at different growth (pre-flowering, full-flowering and post-flowering) stages. Additionally, we investigated the methanolic extract obtained from the aerial parts of the plant material at the full flowering stage in order to determine its inhibitory activity in terms of COX and protein denaturation and evaluate its antimicrobial effects against S. aureus (Gram-positive) and E. coli (Gram-negative) bacteria. Performing GC/MS analysis of HDEO, obtained from the fresh inflorescence heads at the different growth stages, resulted in the identification of 192 constituents. The main class of compounds detected in these three stages comprised aliphatic hydrocarbons and their derivatives, which amounted to 50.04% (pre-flower), 40.28% (full-flower) and 41.34% (post-flower) of the total composition. The oils also contained appreciable amounts of oxygenated terpenoids, primarily sesquiterpenoids and diterpenoids. The pre-flowering stage was dominated by (2E)-hexenal (8.03%) in addition to the oxygenated diterpene (6E,10E)-pseudo phytol (7.54%). The full-flowering stage primarily contained (6E,10E)-pseudo phytol (7.84%), β-bisabolene (7.53%, SH) and the diterpene hydrocarbon dolabradiene (5.50%). The major constituents detected in the HDEO obtained at the post-flowering stage included the oxygenated sesquiterpenoid intermedeol (5.53%), the sesquiterpene hydrocarbon (E)-caryophyllene (5.01%) and (6E,10E)-pseudo phytol (4.47%). The methanolic extract obtained from air-dried aerial parts of E. polyceras displayed more COX-2 inhibition than COX-1 inhibition at a concentration level of 200 µg/mL. The extract exhibited a capacity to inhibit protein denaturation that was comparable with respect to the activity of diclofenac sodium and displayed moderate levels of antimicrobial activity against both bacterial species. The current results demonstrate the need to perform further detailed phytochemical investigations to isolate and characterize active constituents.

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Section: Methodsmentioning

confidence: 99%

Exploring Echinops polyceras Boiss. from Jordan: Essential Oil Composition, COX, Protein Denaturation Inhibitory Power and Antimicrobial Activity of the Alcoholic Extract

Hasan,

Shakya,

Al-Jaber

et al. 2023

Molecules

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“…Essential oils were extracted from fresh aerial parts of the of C. canadensis (inflorescence heads, leaves, and stems) according to the procedure described in the literature [40,41]. Briefly, a weighed sample of the specified fresh organ (Inf: 42.0 g, leaves: 86.21 g, stems: 107.0 g) was coarsely powdered and then subjected to hydro-distillation for 3 h in a Clevenger-type apparatus.…”

Section: Hydro-distillation and Extraction Of Essential Oilsmentioning

confidence: 99%

Conyza canadensis from Jordan: Phytochemical Profiling, Antioxidant, and Antimicrobial Activity Evaluation

Barhoumi,

Shakya,

Al-Fawares

et al. 2024

Molecules

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In this investigation, the chemical composition of the hydro-distilled essential oil (HD-EO), obtained from the fresh aerial parts (inflorescence heads (Inf), leaves (L), and stems (St)) of Conyza canadensis growing wild in Jordan was determined by GC/MS. Additionally, the methanolic extract obtained from the whole aerial parts of C. canadensis (CCM) was examined for its total phenolic content (TPC), total flavonoids content (TFC), DPPH radical scavenging activity, iron chelating activity and was then analyzed with LC-MS/MS for the presence of certain selected phenolic compounds and flavonoids. The GC/MS analysis of CCHD-EOs obtained from the different aerial parts revealed the presence of (2E, 8Z)-matricaria ester as the main component, amounting to 15.4% (Inf), 60.7% (L), and 31.6% (St) of the total content. Oxygenated monoterpenes were the main class of volatile compounds detected in the Inf-CCHD-EO. However, oils obtained from the leaves and stems were rich in polyacetylene derivatives. The evaluation of the CCM extract showed a richness in phenolic content (95.59 ± 0.40 mg GAE/g extract), flavonoids contents (467.0 ± 10.5 mg QE/ g extract), moderate DPPH radical scavenging power (IC50 of 23.75 ± 0.86 µg/mL) and low iron chelating activity (IC50 = 5396.07 ± 15.05 µg/mL). The LC-MS/MS profiling of the CCM extract allowed for the detection of twenty-five phenolic compounds and flavonoids. Results revealed that the CCM extract contained high concentration levels of rosmarinic acid (1441.1 mg/kg plant), in addition to caffeic acid phenethyl ester (231.8 mg/kg plant). An antimicrobial activity assessment of the CCM extract against a set of Gram-positive and Gram-negative bacteria, in addition to two other fungal species including Candida and Cryptococcus, showed significant antibacterial activity of the extract against S. aureus with MIC value of 3.125 µg/mL. The current study is the first phytochemical screening for the essential oil and methanolic extract composition of C. canadensis growing in Jordan, its antioxidant and antimicrobial activity.

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“…In recent years, some experts and scholars at home and abroad have also begun to conduct a large number of studies on the plants of Sedum family, but most of the studies focus on the reports of Notoginseng sedum and Rhodiola rosea, and there are few reports on Sedum spectabile boreau, mainly on the introduction and domestication and breeding of Sedum spectabile boreau, cultivation technology, physiological characteristics, chemical composition and application in garden plant landscape [3] .After in-depth study of Sedum spectabile boreau, we found that it and its extracts contain very complex, rich and diversified biological functional substances, including a variety of natural active alkaloids, steroids, tannins, flavonoids and their derivatives, condensed tannins and cyanogen compounds, etc. [4] ,making flavonoid compounds in the medical effect is very significant [5] .…”

Section: Introductionmentioning

confidence: 99%

Study on Ultrasonic Enzyme-Assisted Extraction Process and Antioxidant Activity of Flavonoids from Sedum spectabile boreau flowers

Na,

Melencion,

Le Le

et al. 2023

BIO Web Conf.

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Using Sedum spectabile boreau flowers as raw material, single factor and Box-Behnken response surface test were used to optimize the extraction conditions of Sedum spectabile boreau flavone assisted by ultrasonic enzymatic method, and the antioxidant activity of Sedum spectabile boreau flavone was analyzed in vitro. The results showed that the optimal extraction conditions of total Sedum spectabile boreau flavonoids were ultrasonic action time of 60 min, ethanol concentration of 35%, liquid-solid ratio (ml /g) was 20:1, and the amount of enzyme (cellulase: pectinase =1:1) was 3.2g.The study on the antioxidant activity of total flavonoids of Sedum spectabile boreau flower showed that its antioxidant capacity was gradually enhanced with the increase of the mass concentration of the test, which had a certain dose-effect relationship.

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Phytochemical and bioactivity evaluation of secondary metabolites and essential oils of Sedum rubens growing wild in Jordan

Cited by 6 publications

References 56 publications

Exploring Echinops polyceras Boiss. from Jordan: Essential Oil Composition, COX, Protein Denaturation Inhibitory Power and Antimicrobial Activity of the Alcoholic Extract

Exploring Echinops polyceras Boiss. from Jordan: Essential Oil Composition, COX, Protein Denaturation Inhibitory Power and Antimicrobial Activity of the Alcoholic Extract

Conyza canadensis from Jordan: Phytochemical Profiling, Antioxidant, and Antimicrobial Activity Evaluation

Study on Ultrasonic Enzyme-Assisted Extraction Process and Antioxidant Activity of Flavonoids from Sedum spectabile boreau flowers

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